Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats
Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use. Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha go...
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| Format: | Article |
| Language: | English |
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AOSIS
2018-02-01
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| Series: | Journal of Medicinal Plants for Economic Development |
| Subjects: | |
| Online Access: | https://jomped.org/index.php/jomped/article/view/45 |
| _version_ | 1811329079929995264 |
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| author | Opeyemi O. Faokunla Oluseyi A. Akinloye Regina N. Ugbaja Abideen I. Adeogun |
| author_facet | Opeyemi O. Faokunla Oluseyi A. Akinloye Regina N. Ugbaja Abideen I. Adeogun |
| author_sort | Opeyemi O. Faokunla |
| collection | DOAJ |
| description | Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use.
Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity in rats using alpha-lipoic acid (α-LA) as standard drug.
Method: Fifty-six male Wistar rats, assigned to eight groups of seven animals each, were treated orally for 28 days as follows - Group 1: only corn oil (Control); Group 2: 20 mg/kg body weight (b.wt) of Cyp; Groups 3, 4 and 5: Cyp + 50 mg/kg b.wt of J. gossypifolia, Cyp + 100 mg/kg b.wt of J. gossypifolia and Cyp + 50 mg/kg b.wt of α-LA, respectively; Groups 6, 7 and 8: J. gossypifolia (50 mg/kg b.wt), J. gossypifolia (100 mg/kg b.wt) and α-LA (50 mg/kg b.wt), respectively. The P53 was assayed for using the plasma, while the micronucleus assay was carried out on the bone marrow.
Results: Results showed that exposure to Cyp significantly increased the number of micronucleated polychromatic erythrocytes (86.70%) as well as p53 concentration in both the liver and plasma (200.00% and 125.53%) compared to the control. Co-administration with methanolic extract of J. gossypifolia leaves significantly reversed these alterations in a dose-dependent manner.
Conclusion: The methanolic extract of J. gossypifolia leaves has the potential to ameliorate Cyp-induced genotoxicity in rats, and also, the toxicological evaluations of the plant extract and α-LA showed that it is relatively safe for use at the doses investigated. |
| first_indexed | 2024-04-13T15:37:30Z |
| format | Article |
| id | doaj.art-5eb5ef3ba78c49ee8ca89cda75cc6c43 |
| institution | Directory Open Access Journal |
| issn | 2519-559X 2616-4809 |
| language | English |
| last_indexed | 2024-04-13T15:37:30Z |
| publishDate | 2018-02-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Journal of Medicinal Plants for Economic Development |
| spelling | doaj.art-5eb5ef3ba78c49ee8ca89cda75cc6c432022-12-22T02:41:14ZengAOSISJournal of Medicinal Plants for Economic Development2519-559X2616-48092018-02-0121e1e510.4102/jomped.v2i1.4512Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in ratsOpeyemi O. Faokunla0Oluseyi A. Akinloye1Regina N. Ugbaja2Abideen I. Adeogun3Department of Biological Sciences, College of Natural and Applied Sciences, Wesley UniversityDepartment of Biochemistry, College of Biological Sciences, Federal University of AgricultureDepartment of Biochemistry, College of Biological Sciences, Federal University of AgricultureDepartment of Chemistry, College of Biological Sciences, Federal University of AgricultureBackground: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use. Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity in rats using alpha-lipoic acid (α-LA) as standard drug. Method: Fifty-six male Wistar rats, assigned to eight groups of seven animals each, were treated orally for 28 days as follows - Group 1: only corn oil (Control); Group 2: 20 mg/kg body weight (b.wt) of Cyp; Groups 3, 4 and 5: Cyp + 50 mg/kg b.wt of J. gossypifolia, Cyp + 100 mg/kg b.wt of J. gossypifolia and Cyp + 50 mg/kg b.wt of α-LA, respectively; Groups 6, 7 and 8: J. gossypifolia (50 mg/kg b.wt), J. gossypifolia (100 mg/kg b.wt) and α-LA (50 mg/kg b.wt), respectively. The P53 was assayed for using the plasma, while the micronucleus assay was carried out on the bone marrow. Results: Results showed that exposure to Cyp significantly increased the number of micronucleated polychromatic erythrocytes (86.70%) as well as p53 concentration in both the liver and plasma (200.00% and 125.53%) compared to the control. Co-administration with methanolic extract of J. gossypifolia leaves significantly reversed these alterations in a dose-dependent manner. Conclusion: The methanolic extract of J. gossypifolia leaves has the potential to ameliorate Cyp-induced genotoxicity in rats, and also, the toxicological evaluations of the plant extract and α-LA showed that it is relatively safe for use at the doses investigated.https://jomped.org/index.php/jomped/article/view/45p53cypermethrinJatropha gossypifoliaalpha-lipoic acidgenotoxicity |
| spellingShingle | Opeyemi O. Faokunla Oluseyi A. Akinloye Regina N. Ugbaja Abideen I. Adeogun Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats Journal of Medicinal Plants for Economic Development p53 cypermethrin Jatropha gossypifolia alpha-lipoic acid genotoxicity |
| title | Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats |
| title_full | Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats |
| title_fullStr | Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats |
| title_full_unstemmed | Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats |
| title_short | Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats |
| title_sort | anticarcinogenic effect of alpha lipoic acid and i jatropha gossypifolia i leaves against cypermethrin induced genotoxicity in rats |
| topic | p53 cypermethrin Jatropha gossypifolia alpha-lipoic acid genotoxicity |
| url | https://jomped.org/index.php/jomped/article/view/45 |
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