Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic
The hepatitis B virus (HBV), belonging to the <i>Hepadnaviridae</i> family, is responsible for a global health concern still in the 21st century. The virus is divided into 10 genotypes, which differ in geographical distribution and in their effect on disease progression and transmission,...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/1/20 |
| _version_ | 1797491339885019136 |
|---|---|
| author | Mariia Logoida Pavol Kristian Andrea Schreiberova Patrícia Denisa Lenártová Veronika Bednárová Elena Hatalová Ivana Hockicková Sylvia Dražilová Peter Jarčuška Martin Janičko Štefan Porhinčák Monika Halánová |
| author_facet | Mariia Logoida Pavol Kristian Andrea Schreiberova Patrícia Denisa Lenártová Veronika Bednárová Elena Hatalová Ivana Hockicková Sylvia Dražilová Peter Jarčuška Martin Janičko Štefan Porhinčák Monika Halánová |
| author_sort | Mariia Logoida |
| collection | DOAJ |
| description | The hepatitis B virus (HBV), belonging to the <i>Hepadnaviridae</i> family, is responsible for a global health concern still in the 21st century. The virus is divided into 10 genotypes, which differ in geographical distribution and in their effect on disease progression and transmission, susceptibility to mutations, and response to treatment. There are many methods for diagnostics of HBV and differentiating its genotypes. Various commercial kits based on real-time polymerase chain reaction (RT PCR) and hybridization available, as well as whole genome sequencing or the sequencing of only individual parts of the genomes. We compared a commercial kit AmpliSens HBV-genotype-FRT, based on RT PCR, with an adapted method of amplification of the surface genomic region combined with Sanger sequencing. In the examined samples we identified the A, B, C, D, and E genotypes. By PCR with Sanger sequencing, the genotypes were determined in all 103 samples, while by using the commercial kit we successfully genotyped only 95 samples, including combined genotypes, which we could not detect by sequencing. |
| first_indexed | 2024-03-10T00:46:05Z |
| format | Article |
| id | doaj.art-5eb70eb63dc84d3fb503633cd31b0625 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T00:46:05Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-5eb70eb63dc84d3fb503633cd31b06252023-11-23T14:59:11ZengMDPI AGPathogens2076-08172021-12-011112010.3390/pathogens11010020Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak RepublicMariia Logoida0Pavol Kristian1Andrea Schreiberova2Patrícia Denisa Lenártová3Veronika Bednárová4Elena Hatalová5Ivana Hockicková6Sylvia Dražilová7Peter Jarčuška8Martin Janičko9Štefan Porhinčák10Monika Halánová11Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, SlovakiaDepartment of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, SlovakiaDepartment of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, SlovakiaDepartment of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, SlovakiaDepartment of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, SlovakiaDepartment of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, SlovakiaDepartment of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, Slovakia2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, SlovakiaCentre of Applied Computer Science, P. J. Safarik University, 040 01 Kosice, SlovakiaDepartment of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, SlovakiaThe hepatitis B virus (HBV), belonging to the <i>Hepadnaviridae</i> family, is responsible for a global health concern still in the 21st century. The virus is divided into 10 genotypes, which differ in geographical distribution and in their effect on disease progression and transmission, susceptibility to mutations, and response to treatment. There are many methods for diagnostics of HBV and differentiating its genotypes. Various commercial kits based on real-time polymerase chain reaction (RT PCR) and hybridization available, as well as whole genome sequencing or the sequencing of only individual parts of the genomes. We compared a commercial kit AmpliSens HBV-genotype-FRT, based on RT PCR, with an adapted method of amplification of the surface genomic region combined with Sanger sequencing. In the examined samples we identified the A, B, C, D, and E genotypes. By PCR with Sanger sequencing, the genotypes were determined in all 103 samples, while by using the commercial kit we successfully genotyped only 95 samples, including combined genotypes, which we could not detect by sequencing.https://www.mdpi.com/2076-0817/11/1/20hepatitis B virusHBV genotypegenotyping methodssequencing |
| spellingShingle | Mariia Logoida Pavol Kristian Andrea Schreiberova Patrícia Denisa Lenártová Veronika Bednárová Elena Hatalová Ivana Hockicková Sylvia Dražilová Peter Jarčuška Martin Janičko Štefan Porhinčák Monika Halánová Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic Pathogens hepatitis B virus HBV genotype genotyping methods sequencing |
| title | Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic |
| title_full | Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic |
| title_fullStr | Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic |
| title_full_unstemmed | Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic |
| title_short | Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic |
| title_sort | comparison of two diagnostic methods for the detection of hepatitis b virus genotypes in the slovak republic |
| topic | hepatitis B virus HBV genotype genotyping methods sequencing |
| url | https://www.mdpi.com/2076-0817/11/1/20 |
| work_keys_str_mv | AT mariialogoida comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT pavolkristian comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT andreaschreiberova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT patriciadenisalenartova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT veronikabednarova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT elenahatalova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT ivanahockickova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT sylviadrazilova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT peterjarcuska comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT martinjanicko comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT stefanporhincak comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic AT monikahalanova comparisonoftwodiagnosticmethodsforthedetectionofhepatitisbvirusgenotypesintheslovakrepublic |