Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid
Solubilization of cholesterol in the intestinal lumen by bile acids and the subsequent formation of mixed micelles is an important step in the absorption of cholesterol. We propose that oxidized fatty acids (ox-FA) may mimic bile acids and form mixed micelles with cholesterol much more efficiently,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2002-06-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520304636 |
| _version_ | 1818931386504970240 |
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| author | Meera Penumetcha Nadya Khan-Merchant Sampath Parthasarathy |
| author_facet | Meera Penumetcha Nadya Khan-Merchant Sampath Parthasarathy |
| author_sort | Meera Penumetcha |
| collection | DOAJ |
| description | Solubilization of cholesterol in the intestinal lumen by bile acids and the subsequent formation of mixed micelles is an important step in the absorption of cholesterol. We propose that oxidized fatty acids (ox-FA) may mimic bile acids and form mixed micelles with cholesterol much more efficiently, as compared with unoxidized fatty acids, thereby increasing there absorption. In an in vitro assay at concentrations of 1, 5, and 10 mM, oxidized linoleic acid (ox-18:2) increased the solubilization of cholesterol (3.06, 8.16, and 15.46 nmol/ml) in a dose dependent manner compared with a 10 mM unoxidized linoleic acid (unox-18:2 at 0.97 nmol/ml). The uptake of cholesterol solubilized in the presence of ox-18:2 by Caco-2 cells and everted rat intestinal sacs was greater (1.78 and 1.95 nmol/ml respectively) as compared with the cholesterol solubilized in the presence of unox-18:2 (0.29 and 0.61 nmol/ml; P = 0.05). In addition, when LDL receptor deficient mice were fed a high fat diet along with ox-18:2 their plasma cholesterol levels were greater than animals fed the high fat diet alone (1290 mg/dl vs. 1549 mg/dl, P = 0.013).From these results, we suggest that ox-FA, by enhancing the solubilization of luminal cholesterol, increases the uptake of cholesterol that might lead to hypercholesterolemia and atherosclerosis. |
| first_indexed | 2024-12-20T04:15:44Z |
| format | Article |
| id | doaj.art-5eb7ceb9039d4e918eb73d6a0ac11714 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T04:15:44Z |
| publishDate | 2002-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5eb7ceb9039d4e918eb73d6a0ac117142022-12-21T19:53:47ZengElsevierJournal of Lipid Research0022-22752002-06-01436895903Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acidMeera Penumetcha0Nadya Khan-Merchant1Sampath Parthasarathy2Department of Gynecology and Obstetrics, Nutrition and Health Sciences Program, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Nutrition and Health Sciences Program, Emory University School of Medicine, Atlanta, GA 30322Department of Gynecology and Obstetrics, Nutrition and Health Sciences Program, Emory University School of Medicine, Atlanta, GA 30322Solubilization of cholesterol in the intestinal lumen by bile acids and the subsequent formation of mixed micelles is an important step in the absorption of cholesterol. We propose that oxidized fatty acids (ox-FA) may mimic bile acids and form mixed micelles with cholesterol much more efficiently, as compared with unoxidized fatty acids, thereby increasing there absorption. In an in vitro assay at concentrations of 1, 5, and 10 mM, oxidized linoleic acid (ox-18:2) increased the solubilization of cholesterol (3.06, 8.16, and 15.46 nmol/ml) in a dose dependent manner compared with a 10 mM unoxidized linoleic acid (unox-18:2 at 0.97 nmol/ml). The uptake of cholesterol solubilized in the presence of ox-18:2 by Caco-2 cells and everted rat intestinal sacs was greater (1.78 and 1.95 nmol/ml respectively) as compared with the cholesterol solubilized in the presence of unox-18:2 (0.29 and 0.61 nmol/ml; P = 0.05). In addition, when LDL receptor deficient mice were fed a high fat diet along with ox-18:2 their plasma cholesterol levels were greater than animals fed the high fat diet alone (1290 mg/dl vs. 1549 mg/dl, P = 0.013).From these results, we suggest that ox-FA, by enhancing the solubilization of luminal cholesterol, increases the uptake of cholesterol that might lead to hypercholesterolemia and atherosclerosis.http://www.sciencedirect.com/science/article/pii/S0022227520304636bile acidsatherosclerosislipid peroxidesoxidized fatty acids |
| spellingShingle | Meera Penumetcha Nadya Khan-Merchant Sampath Parthasarathy Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid Journal of Lipid Research bile acids atherosclerosis lipid peroxides oxidized fatty acids |
| title | Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| title_full | Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| title_fullStr | Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| title_full_unstemmed | Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| title_short | Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| title_sort | enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid |
| topic | bile acids atherosclerosis lipid peroxides oxidized fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520304636 |
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