Seroprevalence of porcine cysticercosis in Maharashtra
Porcine cysticercosis is caused by Cysticercus cellulosae, a larval stage of parasite Taenia solium. Humans get infected by consuming eggs of T. solium through contamination of food and water. Owing to economic and zoonotic importance of porcine cysticercosis in India, a research plan was designed t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2019-04-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/89090 |
| _version_ | 1797896679252295680 |
|---|---|
| author | NILAM WAVHAL ASHISH PATURKAR VILAS VAIDYA RUPESH WAGHMARE RAVINDRA ZENDE SHILPA MOON |
| author_facet | NILAM WAVHAL ASHISH PATURKAR VILAS VAIDYA RUPESH WAGHMARE RAVINDRA ZENDE SHILPA MOON |
| author_sort | NILAM WAVHAL |
| collection | DOAJ |
| description | Porcine cysticercosis is caused by Cysticercus cellulosae, a larval stage of parasite Taenia solium. Humans get infected by consuming eggs of T. solium through contamination of food and water. Owing to economic and zoonotic importance of porcine cysticercosis in India, a research plan was designed to study seroprevalence of cysticercosis in Maharashtra. Blood samples (172) were collected from different regions. The seroprevalence of cysticercosis by ELISA, FTA and western blot was 8.5%, 7.5% and 6.98%, respectively. Cohen’s kappa coefficient was used to analyse for the percentage of agreement with meat inspection test. The sensitivity and specificity between ELISA and FTA (k=0.958) showed no significant difference. The study indicates that ELISA and FTA were more sensitive than western blot. Western blot of whole cyst antigen revealed immunoreactivity at 42 to 250 kDa bands. The seroprevalence of porcine cysticercosis in Maharashtra had increased significantly. FTA is easier to perform and faster than ELISA test. This simple test appears to be suitable for practical use at field level, especially for largescale ante-mortem screening of pigs against cysticercosis. |
| first_indexed | 2024-04-10T07:46:35Z |
| format | Article |
| id | doaj.art-5eb854b873fe4162b9abd9aee60af074 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:46:35Z |
| publishDate | 2019-04-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-5eb854b873fe4162b9abd9aee60af0742023-02-23T10:34:43ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-04-0189410.56093/ijans.v89i4.89090Seroprevalence of porcine cysticercosis in MaharashtraNILAM WAVHAL0ASHISH PATURKAR1VILAS VAIDYA2RUPESH WAGHMARE3RAVINDRA ZENDE4SHILPA MOON5Research Fellow, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, PunjabVice Chancellor, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra 440 006 IndiaAssistant Professor, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra 440 006 IndiaAssistant Professor, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra 440 006 IndiaProfessor, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra 440 006 IndiaPhD Scholar, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra 440 006 IndiaPorcine cysticercosis is caused by Cysticercus cellulosae, a larval stage of parasite Taenia solium. Humans get infected by consuming eggs of T. solium through contamination of food and water. Owing to economic and zoonotic importance of porcine cysticercosis in India, a research plan was designed to study seroprevalence of cysticercosis in Maharashtra. Blood samples (172) were collected from different regions. The seroprevalence of cysticercosis by ELISA, FTA and western blot was 8.5%, 7.5% and 6.98%, respectively. Cohen’s kappa coefficient was used to analyse for the percentage of agreement with meat inspection test. The sensitivity and specificity between ELISA and FTA (k=0.958) showed no significant difference. The study indicates that ELISA and FTA were more sensitive than western blot. Western blot of whole cyst antigen revealed immunoreactivity at 42 to 250 kDa bands. The seroprevalence of porcine cysticercosis in Maharashtra had increased significantly. FTA is easier to perform and faster than ELISA test. This simple test appears to be suitable for practical use at field level, especially for largescale ante-mortem screening of pigs against cysticercosis.https://epubs.icar.org.in/index.php/IJAnS/article/view/89090CysticercosisELISAFTAPorcineWestern blot |
| spellingShingle | NILAM WAVHAL ASHISH PATURKAR VILAS VAIDYA RUPESH WAGHMARE RAVINDRA ZENDE SHILPA MOON Seroprevalence of porcine cysticercosis in Maharashtra Indian Journal of Animal Sciences Cysticercosis ELISA FTA Porcine Western blot |
| title | Seroprevalence of porcine cysticercosis in Maharashtra |
| title_full | Seroprevalence of porcine cysticercosis in Maharashtra |
| title_fullStr | Seroprevalence of porcine cysticercosis in Maharashtra |
| title_full_unstemmed | Seroprevalence of porcine cysticercosis in Maharashtra |
| title_short | Seroprevalence of porcine cysticercosis in Maharashtra |
| title_sort | seroprevalence of porcine cysticercosis in maharashtra |
| topic | Cysticercosis ELISA FTA Porcine Western blot |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/89090 |
| work_keys_str_mv | AT nilamwavhal seroprevalenceofporcinecysticercosisinmaharashtra AT ashishpaturkar seroprevalenceofporcinecysticercosisinmaharashtra AT vilasvaidya seroprevalenceofporcinecysticercosisinmaharashtra AT rupeshwaghmare seroprevalenceofporcinecysticercosisinmaharashtra AT ravindrazende seroprevalenceofporcinecysticercosisinmaharashtra AT shilpamoon seroprevalenceofporcinecysticercosisinmaharashtra |