Possible existence of a system similar to bacterial restriction–modification in plants
The bacterial restriction–modification (R-M) system has evolved as a defense mechanism against infectious phages and other types of integration of foreign DNA fragments into the host chromosome through recombination. In this study, we identified two endonucleases (wheat endonuclease 1-WEN1 and wheat...
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| Format: | Article |
| Language: | English |
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AIMS Press
2020-12-01
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| Series: | AIMS Molecular Science |
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| Online Access: | http://www.aimspress.com/article/10.3934/molsci.2020020?viewType=HTML |
| _version_ | 1828524889843171328 |
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| author | Larisa I. Fedoreyeva Boris F. Vanyushin |
| author_facet | Larisa I. Fedoreyeva Boris F. Vanyushin |
| author_sort | Larisa I. Fedoreyeva |
| collection | DOAJ |
| description | The bacterial restriction–modification (R-M) system has evolved as a defense mechanism against infectious phages and other types of integration of foreign DNA fragments into the host chromosome through recombination. In this study, we identified two endonucleases (wheat endonuclease 1-WEN1 and wheat endonuclease 2-WEN2) and the adenine-DNA methyltransferase WAD (involved in mitochondrial DNA methylation) in the vesicular fraction of coleoptiles of wheat Triticum aestivum. WEN1 and WEN2 have multidirectional sensitivity to DNA methylation and to the presence of S-adenosyl-L-methionine and Ca2+ ions, which suggests their participation in the protection of mitochondrial DNA, similar to bacterial DNA. WEN2 has a GAT recognition site, which is part of the methylation site of adenine DNA methyltransferase (WAD-TGATCA) and hydrolyzes the CG (GC) bond upstream of the recognition site only if it is not methylated. Based on the interrelations between the two enzymes, we propose that an R-M system similar to that of the type IV observed in bacteria may exist in plants. |
| first_indexed | 2024-12-11T20:56:00Z |
| format | Article |
| id | doaj.art-5eb9373d9328488786e78fead251a136 |
| institution | Directory Open Access Journal |
| issn | 2372-0301 |
| language | English |
| last_indexed | 2024-12-11T20:56:00Z |
| publishDate | 2020-12-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Molecular Science |
| spelling | doaj.art-5eb9373d9328488786e78fead251a1362022-12-22T00:51:06ZengAIMS PressAIMS Molecular Science2372-03012020-12-017439641310.3934/molsci.2020020Possible existence of a system similar to bacterial restriction–modification in plantsLarisa I. Fedoreyeva0 Boris F. Vanyushin11. All-Russian Research Institute of Agricultural Biotechnology RAS, 127550 Moscow, Timiryazevskaya 42, Russia1. All-Russian Research Institute of Agricultural Biotechnology RAS, 127550 Moscow, Timiryazevskaya 42, Russia 2. A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Leninskie Gory1, building 40, RussiaThe bacterial restriction–modification (R-M) system has evolved as a defense mechanism against infectious phages and other types of integration of foreign DNA fragments into the host chromosome through recombination. In this study, we identified two endonucleases (wheat endonuclease 1-WEN1 and wheat endonuclease 2-WEN2) and the adenine-DNA methyltransferase WAD (involved in mitochondrial DNA methylation) in the vesicular fraction of coleoptiles of wheat Triticum aestivum. WEN1 and WEN2 have multidirectional sensitivity to DNA methylation and to the presence of S-adenosyl-L-methionine and Ca2+ ions, which suggests their participation in the protection of mitochondrial DNA, similar to bacterial DNA. WEN2 has a GAT recognition site, which is part of the methylation site of adenine DNA methyltransferase (WAD-TGATCA) and hydrolyzes the CG (GC) bond upstream of the recognition site only if it is not methylated. Based on the interrelations between the two enzymes, we propose that an R-M system similar to that of the type IV observed in bacteria may exist in plants.http://www.aimspress.com/article/10.3934/molsci.2020020?viewType=HTMLendonucleasewen1wen2adenine dna methyltransferasewadrestriction–modification systemtriticum aestivum |
| spellingShingle | Larisa I. Fedoreyeva Boris F. Vanyushin Possible existence of a system similar to bacterial restriction–modification in plants AIMS Molecular Science endonuclease wen1 wen2 adenine dna methyltransferase wad restriction–modification system triticum aestivum |
| title | Possible existence of a system similar to bacterial restriction–modification in plants |
| title_full | Possible existence of a system similar to bacterial restriction–modification in plants |
| title_fullStr | Possible existence of a system similar to bacterial restriction–modification in plants |
| title_full_unstemmed | Possible existence of a system similar to bacterial restriction–modification in plants |
| title_short | Possible existence of a system similar to bacterial restriction–modification in plants |
| title_sort | possible existence of a system similar to bacterial restriction modification in plants |
| topic | endonuclease wen1 wen2 adenine dna methyltransferase wad restriction–modification system triticum aestivum |
| url | http://www.aimspress.com/article/10.3934/molsci.2020020?viewType=HTML |
| work_keys_str_mv | AT larisaifedoreyeva possibleexistenceofasystemsimilartobacterialrestrictionmodificationinplants AT borisfvanyushin possibleexistenceofasystemsimilartobacterialrestrictionmodificationinplants |