Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm
We evaluated the effects of feeding 60% dried corn distillers’ grains plus solubles (DDGS) or the equivalent sulfur as CaSO<sub>4</sub> on sperm characteristics and transcript abundance. Thirty-six half-sibling Angus bulls (256 ± 8.5 d; initial BW = 320 ± 2.7 kg) were assigned to one of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Ruminants |
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| Online Access: | https://www.mdpi.com/2673-933X/3/4/26 |
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| author | Cierrah J. Kassetas Tom W. Geary Abby L. Zezeski Joel S. Caton James D. Kirsch Sheri T. Dorsam Wellison J. S. Diniz Kacie L. McCarthy Matthew S. Crouse Kevin K. Sedivec Bryan W. Neville Carl R. Dahlen |
| author_facet | Cierrah J. Kassetas Tom W. Geary Abby L. Zezeski Joel S. Caton James D. Kirsch Sheri T. Dorsam Wellison J. S. Diniz Kacie L. McCarthy Matthew S. Crouse Kevin K. Sedivec Bryan W. Neville Carl R. Dahlen |
| author_sort | Cierrah J. Kassetas |
| collection | DOAJ |
| description | We evaluated the effects of feeding 60% dried corn distillers’ grains plus solubles (DDGS) or the equivalent sulfur as CaSO<sub>4</sub> on sperm characteristics and transcript abundance. Thirty-six half-sibling Angus bulls (256 ± 8.5 d; initial BW = 320 ± 2.7 kg) were assigned to one of three treatments: (1) 60% concentrate as corn (CON); (2) 60% DDGS as corn replacement (60DDGS); and (3) CON diet + equivalent sulfur of 60DDGS added as CaSO<sub>4</sub> (SULF). The acrosome/cell membrane integrity, mitochondrial energy potential, oxidation status, DNA integrity, and zinc signatures were analyzed via flow cytometry. Sperm-specific gene expression was assessed via RNA sequencing. The flow cytometry data were analyzed using PROC MIXED in SAS to determine the effects of treatment. Pairwise comparisons based on edgeR were used to identify differentially expressed genes. The percentage of polarized mitochondria tended to be greater (<i>p</i> = 0.08) for SULF compared with CON and 60DDGS. Protamine 1 was upregulated (<i>p</i> < 0.01; FDR = 0.10) in 60DDGS compared to CON. Zinc signature 1 in 60DDGS and SULF was reduced (<i>p</i> = 0.03) compared to CON. This study suggests that feeding bulls diets containing 60% DDGS had little effect on DNA integrity and gene expression. |
| first_indexed | 2024-03-08T20:24:03Z |
| format | Article |
| id | doaj.art-5ebccc7996d94d5986de7005b4c59b28 |
| institution | Directory Open Access Journal |
| issn | 2673-933X |
| language | English |
| last_indexed | 2024-03-08T20:24:03Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Ruminants |
| spelling | doaj.art-5ebccc7996d94d5986de7005b4c59b282023-12-22T14:39:40ZengMDPI AGRuminants2673-933X2023-10-013428629810.3390/ruminants3040026Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull SpermCierrah J. Kassetas0Tom W. Geary1Abby L. Zezeski2Joel S. Caton3James D. Kirsch4Sheri T. Dorsam5Wellison J. S. Diniz6Kacie L. McCarthy7Matthew S. Crouse8Kevin K. Sedivec9Bryan W. Neville10Carl R. Dahlen11Center for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USAFort Keogh Livestock and Range Research Laboratory, United States Department of Agriculture—Agriculture Research Service, Miles City, MT 59301, USAFort Keogh Livestock and Range Research Laboratory, United States Department of Agriculture—Agriculture Research Service, Miles City, MT 59301, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USADepartment of Animal Science, Auburn University, Auburn, AL 36849, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USACentral Grasslands Research and Extension Center, North Dakota State University, Streeter, ND 58483, USACarrington Research Extension Center, North Dakota State University, Carrington, ND 58421, USACenter for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USAWe evaluated the effects of feeding 60% dried corn distillers’ grains plus solubles (DDGS) or the equivalent sulfur as CaSO<sub>4</sub> on sperm characteristics and transcript abundance. Thirty-six half-sibling Angus bulls (256 ± 8.5 d; initial BW = 320 ± 2.7 kg) were assigned to one of three treatments: (1) 60% concentrate as corn (CON); (2) 60% DDGS as corn replacement (60DDGS); and (3) CON diet + equivalent sulfur of 60DDGS added as CaSO<sub>4</sub> (SULF). The acrosome/cell membrane integrity, mitochondrial energy potential, oxidation status, DNA integrity, and zinc signatures were analyzed via flow cytometry. Sperm-specific gene expression was assessed via RNA sequencing. The flow cytometry data were analyzed using PROC MIXED in SAS to determine the effects of treatment. Pairwise comparisons based on edgeR were used to identify differentially expressed genes. The percentage of polarized mitochondria tended to be greater (<i>p</i> = 0.08) for SULF compared with CON and 60DDGS. Protamine 1 was upregulated (<i>p</i> < 0.01; FDR = 0.10) in 60DDGS compared to CON. Zinc signature 1 in 60DDGS and SULF was reduced (<i>p</i> = 0.03) compared to CON. This study suggests that feeding bulls diets containing 60% DDGS had little effect on DNA integrity and gene expression.https://www.mdpi.com/2673-933X/3/4/26beef bullsdistillers’ grains plus solublesDNA integritygene sequencingsperm |
| spellingShingle | Cierrah J. Kassetas Tom W. Geary Abby L. Zezeski Joel S. Caton James D. Kirsch Sheri T. Dorsam Wellison J. S. Diniz Kacie L. McCarthy Matthew S. Crouse Kevin K. Sedivec Bryan W. Neville Carl R. Dahlen Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm Ruminants beef bulls distillers’ grains plus solubles DNA integrity gene sequencing sperm |
| title | Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm |
| title_full | Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm |
| title_fullStr | Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm |
| title_full_unstemmed | Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm |
| title_short | Effects of Feeding 60% Dried Corn Distillers’ Grains or the Equivalent Sulfur as CaSO<sub>4</sub> on DNA Integrity and Gene Expression in Yearling Angus Bull Sperm |
| title_sort | effects of feeding 60 dried corn distillers grains or the equivalent sulfur as caso sub 4 sub on dna integrity and gene expression in yearling angus bull sperm |
| topic | beef bulls distillers’ grains plus solubles DNA integrity gene sequencing sperm |
| url | https://www.mdpi.com/2673-933X/3/4/26 |
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