Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats
Obesity is one of the most serious public health challenges. Recently, we found that flaxseed polysaccharides (FPs) had an anti-obesity effect through promoting lipid metabolism, but the obesity-inhibiting pathway of FP is still unclear. In this study, after FP intervention in an obese rat model, a...
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/13/1991 |
| _version_ | 1827654716661694464 |
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| author | Hua Wei Xiaohong Lin Liu Liu Xichun Peng |
| author_facet | Hua Wei Xiaohong Lin Liu Liu Xichun Peng |
| author_sort | Hua Wei |
| collection | DOAJ |
| description | Obesity is one of the most serious public health challenges. Recently, we found that flaxseed polysaccharides (FPs) had an anti-obesity effect through promoting lipid metabolism, but the obesity-inhibiting pathway of FP is still unclear. In this study, after FP intervention in an obese rat model, a transcriptome study was performed to further investigate how FP intervention alters the gene expression of colonic epithelial tissues (CETs). The results showed that there were 3785 genes differentially expressed due to the FP intervention, namely 374 downregulated and 3411 upregulated genes. After analyzing all the differentially expressed genes, two classical KEGG pathways were found to be related to obesity, namely the PPAR-signaling pathway and energy metabolism, involving genes <i>Fabp1–5</i>, <i>Lpl</i>, <i>Gyk</i>, <i>Qqp7</i>, <i>Pparg</i>, <i>Rxrg</i>, <i>Acsl1</i>, <i>Acsl4</i>, <i>Acsl6</i>, <i>Cpt1c, Car1–4, Ca5b, Car8, Car12–14, Cps1, Ndufa4l2, Cox6b2, Atp6v1g2, Ndufa4l2</i> and <i>Cox4i2</i>. QRT-PCR results showed a consistent expression trend. Our results indicate that FP promotes lipid metabolism by changing the expression of some key genes of CETs, thus inhibiting obesity. |
| first_indexed | 2024-03-09T21:52:47Z |
| format | Article |
| id | doaj.art-5ec2fa8c6a6c4b6484f417924068ab84 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:52:47Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-5ec2fa8c6a6c4b6484f417924068ab842023-11-23T20:02:44ZengMDPI AGFoods2304-81582022-07-011113199110.3390/foods11131991Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese RatsHua Wei0Xiaohong Lin1Liu Liu2Xichun Peng3College of Food Science and Engineering, Nanchang Univeristy, Nanchang 330096, ChinaDepartment of Food Science and Engineering, Jinan University, Guangzhou 510632, ChinaDepartment of Food Science and Engineering, Jinan University, Guangzhou 510632, ChinaDepartment of Food Science and Engineering, Jinan University, Guangzhou 510632, ChinaObesity is one of the most serious public health challenges. Recently, we found that flaxseed polysaccharides (FPs) had an anti-obesity effect through promoting lipid metabolism, but the obesity-inhibiting pathway of FP is still unclear. In this study, after FP intervention in an obese rat model, a transcriptome study was performed to further investigate how FP intervention alters the gene expression of colonic epithelial tissues (CETs). The results showed that there were 3785 genes differentially expressed due to the FP intervention, namely 374 downregulated and 3411 upregulated genes. After analyzing all the differentially expressed genes, two classical KEGG pathways were found to be related to obesity, namely the PPAR-signaling pathway and energy metabolism, involving genes <i>Fabp1–5</i>, <i>Lpl</i>, <i>Gyk</i>, <i>Qqp7</i>, <i>Pparg</i>, <i>Rxrg</i>, <i>Acsl1</i>, <i>Acsl4</i>, <i>Acsl6</i>, <i>Cpt1c, Car1–4, Ca5b, Car8, Car12–14, Cps1, Ndufa4l2, Cox6b2, Atp6v1g2, Ndufa4l2</i> and <i>Cox4i2</i>. QRT-PCR results showed a consistent expression trend. Our results indicate that FP promotes lipid metabolism by changing the expression of some key genes of CETs, thus inhibiting obesity.https://www.mdpi.com/2304-8158/11/13/1991flaxseed polysaccharidecolonic gene expressionlipid metabolism |
| spellingShingle | Hua Wei Xiaohong Lin Liu Liu Xichun Peng Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats Foods flaxseed polysaccharide colonic gene expression lipid metabolism |
| title | Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats |
| title_full | Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats |
| title_fullStr | Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats |
| title_full_unstemmed | Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats |
| title_short | Flaxseed Polysaccharide Alters Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rats |
| title_sort | flaxseed polysaccharide alters colonic gene expression of lipid metabolism and energy metabolism in obese rats |
| topic | flaxseed polysaccharide colonic gene expression lipid metabolism |
| url | https://www.mdpi.com/2304-8158/11/13/1991 |
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