Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology
Background: Magnetoliposomes have pronounced signal-enhancing effect on T1-weighted (T1w) images of the liver using qualitative analysis which may be benefical for demonstrating peritumoral vasculature. Purpose: To correlate peri-tumoral vasculature (ring-enhancement) surrounding colorectal liver me...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Ubiquity Press
2005-02-01
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| Series: | Journal of the Belgian Society of Radiology |
| Subjects: | |
| Online Access: | https://www.jbsr.be/articles/147 |
| _version_ | 1811256386209710080 |
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| author | K Coenegrachts J van Werven L ter Beek Z Mzallassi M Bögels Th van Gulik I Van Den Berghe A Nederveen J Stoker H Rigauts S Soenen M De Cuyper |
| author_facet | K Coenegrachts J van Werven L ter Beek Z Mzallassi M Bögels Th van Gulik I Van Den Berghe A Nederveen J Stoker H Rigauts S Soenen M De Cuyper |
| author_sort | K Coenegrachts |
| collection | DOAJ |
| description | Background: Magnetoliposomes have pronounced signal-enhancing effect on T1-weighted (T1w) images of the liver using qualitative analysis which may be benefical for demonstrating peritumoral vasculature. Purpose: To correlate peri-tumoral vasculature (ring-enhancement) surrounding colorectal liver metastases after injection of magnetoliposomes using T1-weighted (T1w) imaging with histopathology in a rat model. Material and Methods: All experiments were approved by the responsible Animal Care Committee. Three rats injected with CC531 coloncarcinoma cells in the portal vein were imaged at 3T using a small diameter four channel coil. The presence of liver metastases, signal intensity changes within intrahepatic vessels, peri-tumoral vasculature (ringenhancement) surrounding liver metastases on T1w imaging and histopathology, and the histopathological distribution of iron particles were evaluated. SS SE-EPI and T1w GE sequences were used. Images were evaluated qualitatively and MRI findings were correlated with histopathology. Results: Fifteen liver metastases were present which were all detected at MRI (mean diameter 2.4 mm (SD 0.8 mm, range 1.5 -4.7 mm)). Ring-enhancement surrounding liver metastases at contrast-enhanced T1w GE sequences was present in all liver metastases. Correlation with histopathology showed the corresponding presence of dilated sinusoids filled with iron particles surrounding the liver metastases. Conclusion: Blood-pooling of iron oxide particles within magnetoliposomes was demonstrated with increased and hyperintensity of vessels after injection of magnetoliposomes. Qualitatively, ring-enhancement surrounding the liver metastases was seen on T1w imaging and corresponded histopathologically with the presence of iron particles (magnetoliposomes) within the dilated sinusoids surrounding the liver metastases. |
| first_indexed | 2024-04-12T17:39:34Z |
| format | Article |
| id | doaj.art-5ec322ba7ea644b0973298b884c0db3f |
| institution | Directory Open Access Journal |
| issn | 2514-8281 |
| language | English |
| last_indexed | 2024-04-12T17:39:34Z |
| publishDate | 2005-02-01 |
| publisher | Ubiquity Press |
| record_format | Article |
| series | Journal of the Belgian Society of Radiology |
| spelling | doaj.art-5ec322ba7ea644b0973298b884c0db3f2022-12-22T03:22:50ZengUbiquity PressJournal of the Belgian Society of Radiology2514-82812005-02-01932879110.5334/jbr-btr.147147Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathologyK Coenegrachts0J van Werven1L ter Beek2Z Mzallassi3M Bögels4Th van Gulik5I Van Den Berghe6A Nederveen7J Stoker8H Rigauts9S Soenen10M De Cuyper11Department of Radiology, AZ St.-Jan Brugge-Oostende AV, Bruges, BelgiumDepartment of Radiology, Academic Medical Center, Amsterdam, The NetherlandsPhilips Healthcare, Eindhoven, The NetherlandsDepartment of Surgery/Surgical Laboratory, Academic Medical Center, Amsterdam, The NetherlandsDepartment of Molecular Cell biology and Immunology, VU University Medical Center, Amsterdam, The NetherlandsDepartment of Surgery/Surgical Laboratory, Academic Medical Center, Amsterdam, The NetherlandsDepartment of Pathology, AZ St.-Jan Brugge-Oostende AV, Bruges, BelgiumDepartment of Radiology, Academic Medical Center, Amsterdam, The NetherlandsDepartment of Radiology, Academic Medical Center, Amsterdam, The NetherlandsDepartment of Radiology, AZ St.-Jan Brugge-Oostende AV, Bruges, BelgiumInterdisciplinary Research Center, Catholic University Leuven-Campus Kortrijk, Kortrijk, BelgiumInterdisciplinary Research Center, Catholic University Leuven-Campus Kortrijk, Kortrijk, BelgiumBackground: Magnetoliposomes have pronounced signal-enhancing effect on T1-weighted (T1w) images of the liver using qualitative analysis which may be benefical for demonstrating peritumoral vasculature. Purpose: To correlate peri-tumoral vasculature (ring-enhancement) surrounding colorectal liver metastases after injection of magnetoliposomes using T1-weighted (T1w) imaging with histopathology in a rat model. Material and Methods: All experiments were approved by the responsible Animal Care Committee. Three rats injected with CC531 coloncarcinoma cells in the portal vein were imaged at 3T using a small diameter four channel coil. The presence of liver metastases, signal intensity changes within intrahepatic vessels, peri-tumoral vasculature (ringenhancement) surrounding liver metastases on T1w imaging and histopathology, and the histopathological distribution of iron particles were evaluated. SS SE-EPI and T1w GE sequences were used. Images were evaluated qualitatively and MRI findings were correlated with histopathology. Results: Fifteen liver metastases were present which were all detected at MRI (mean diameter 2.4 mm (SD 0.8 mm, range 1.5 -4.7 mm)). Ring-enhancement surrounding liver metastases at contrast-enhanced T1w GE sequences was present in all liver metastases. Correlation with histopathology showed the corresponding presence of dilated sinusoids filled with iron particles surrounding the liver metastases. Conclusion: Blood-pooling of iron oxide particles within magnetoliposomes was demonstrated with increased and hyperintensity of vessels after injection of magnetoliposomes. Qualitatively, ring-enhancement surrounding the liver metastases was seen on T1w imaging and corresponded histopathologically with the presence of iron particles (magnetoliposomes) within the dilated sinusoids surrounding the liver metastases.https://www.jbsr.be/articles/147Liver neoplasmssecondary – Neoplasmsblood supply |
| spellingShingle | K Coenegrachts J van Werven L ter Beek Z Mzallassi M Bögels Th van Gulik I Van Den Berghe A Nederveen J Stoker H Rigauts S Soenen M De Cuyper Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology Journal of the Belgian Society of Radiology Liver neoplasms secondary – Neoplasms blood supply |
| title | Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology |
| title_full | Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology |
| title_fullStr | Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology |
| title_full_unstemmed | Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology |
| title_short | Evaluation of peri-tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats: correlation with 3t mri and histopathology |
| title_sort | evaluation of peri tumoral vessels surrounding colorectal liver metastases after intravenous injection of extruded magnetoliposomes in rats correlation with 3t mri and histopathology |
| topic | Liver neoplasms secondary – Neoplasms blood supply |
| url | https://www.jbsr.be/articles/147 |
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