A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research
Abstract Objective: Duodenoscopy-associated infections and outbreaks are reported globally despite strict adherence to duodenoscope reprocessing protocols. Therefore, new developments in the reprocessing procedure are needed. Design: We evaluated a novel dynamic flow model for an additional clea...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2022-01-01
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| Series: | Antimicrobial Stewardship & Healthcare Epidemiology |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2732494X22002947/type/journal_article |
| _version_ | 1811157161174106112 |
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| author | Maarten Heuvelmans Willem Woudstra Herman F. Wunderink Jan F. Monkelbaan Henny C. van der Mei |
| author_facet | Maarten Heuvelmans Willem Woudstra Herman F. Wunderink Jan F. Monkelbaan Henny C. van der Mei |
| author_sort | Maarten Heuvelmans |
| collection | DOAJ |
| description |
Abstract
Objective:
Duodenoscopy-associated infections and outbreaks are reported globally despite strict adherence to duodenoscope reprocessing protocols. Therefore, new developments in the reprocessing procedure are needed.
Design:
We evaluated a novel dynamic flow model for an additional cleaning step between precleaning and manual cleaning in the reprocessing procedure.
Methods:
A parallel plate flow chamber with a fluorinated ethylene propylene bottom plate was used to mimic the duodenoscope channels. The flow chamber was inoculated with a suspension containing Klebsiella pneumoniae to simulate bacterial contamination during a duodenoscopic procedure. After inoculation the flow chamber was flushed with a detergent mimicking precleaning. Subsequently the flow chamber was subjected to different interventions: flow with phosphate-buffered saline (PBS), flow with 2 commercial detergents, flow with sodium dodecyl sulfate with 3 different concentrations, and flow with microbubbles. Adhering bacteria were counted using phase-contrast microscopy throughout the experiment, and finally, bacterial viability was assessed.
Results:
During precleaning both PBS and 1% (v/v) Neodisher Mediclean Forte were able to desorb bacteria, but neither proved superior. After precleaning only sodium dodecyl sulfate could desorb bacteria.
Conclusions:
Flushing during precleaning is an essential step for reducing adhering luminal bacteria, and sodium dodecyl sulfate is a promising detergent for bacterial desorption from duodenoscope channels after precleaning.
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| first_indexed | 2024-04-10T05:02:22Z |
| format | Article |
| id | doaj.art-5ec3239b98d747ddac80d1ae3b687ddf |
| institution | Directory Open Access Journal |
| issn | 2732-494X |
| language | English |
| last_indexed | 2024-04-10T05:02:22Z |
| publishDate | 2022-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Antimicrobial Stewardship & Healthcare Epidemiology |
| spelling | doaj.art-5ec3239b98d747ddac80d1ae3b687ddf2023-03-09T12:28:17ZengCambridge University PressAntimicrobial Stewardship & Healthcare Epidemiology2732-494X2022-01-01210.1017/ash.2022.294A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational researchMaarten Heuvelmans0https://orcid.org/0000-0002-2381-7086Willem Woudstra1https://orcid.org/0000-0002-7212-3443Herman F. Wunderink2https://orcid.org/0000-0001-9491-0790Jan F. Monkelbaan3https://orcid.org/0000-0001-9030-059XHenny C. van der Mei4Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, Groningen, The NetherlandsDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Utrecht, Utrecht, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, Groningen, The Netherlands Abstract Objective: Duodenoscopy-associated infections and outbreaks are reported globally despite strict adherence to duodenoscope reprocessing protocols. Therefore, new developments in the reprocessing procedure are needed. Design: We evaluated a novel dynamic flow model for an additional cleaning step between precleaning and manual cleaning in the reprocessing procedure. Methods: A parallel plate flow chamber with a fluorinated ethylene propylene bottom plate was used to mimic the duodenoscope channels. The flow chamber was inoculated with a suspension containing Klebsiella pneumoniae to simulate bacterial contamination during a duodenoscopic procedure. After inoculation the flow chamber was flushed with a detergent mimicking precleaning. Subsequently the flow chamber was subjected to different interventions: flow with phosphate-buffered saline (PBS), flow with 2 commercial detergents, flow with sodium dodecyl sulfate with 3 different concentrations, and flow with microbubbles. Adhering bacteria were counted using phase-contrast microscopy throughout the experiment, and finally, bacterial viability was assessed. Results: During precleaning both PBS and 1% (v/v) Neodisher Mediclean Forte were able to desorb bacteria, but neither proved superior. After precleaning only sodium dodecyl sulfate could desorb bacteria. Conclusions: Flushing during precleaning is an essential step for reducing adhering luminal bacteria, and sodium dodecyl sulfate is a promising detergent for bacterial desorption from duodenoscope channels after precleaning. https://www.cambridge.org/core/product/identifier/S2732494X22002947/type/journal_article |
| spellingShingle | Maarten Heuvelmans Willem Woudstra Herman F. Wunderink Jan F. Monkelbaan Henny C. van der Mei A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research Antimicrobial Stewardship & Healthcare Epidemiology |
| title | A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| title_full | A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| title_fullStr | A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| title_full_unstemmed | A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| title_short | A dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| title_sort | dynamic flow model mimicking duodenoscope reprocessing after bacterial contamination for translational research |
| url | https://www.cambridge.org/core/product/identifier/S2732494X22002947/type/journal_article |
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