TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis)
Abstract Alternative splicing (AS) increases the diversity of transcripts and proteins through the selection of different splice sites and plays an important role in the growth, development and stress tolerance of plants. With the release of the reference genome of the tea plant (Camellia sinensis)...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03065-8 |
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| author | Xiaozeng Mi Yi Yue Mengsha Tang Yanlin An Hui Xie Dahe Qiao Zhiyu Ma Shengrui Liu Chaoling Wei |
| author_facet | Xiaozeng Mi Yi Yue Mengsha Tang Yanlin An Hui Xie Dahe Qiao Zhiyu Ma Shengrui Liu Chaoling Wei |
| author_sort | Xiaozeng Mi |
| collection | DOAJ |
| description | Abstract Alternative splicing (AS) increases the diversity of transcripts and proteins through the selection of different splice sites and plays an important role in the growth, development and stress tolerance of plants. With the release of the reference genome of the tea plant (Camellia sinensis) and the development of transcriptome sequencing, researchers have reported the existence of AS in tea plants. However, there is a lack of a platform, centered on different RNA-seq datasets, that provides comprehensive information on AS. To facilitate access to information on AS and reveal the molecular function of AS in tea plants, we established the first comprehensive AS database for tea plants (TeaAS, http://www.teaas.cn/index.php ). In this study, 3.96 Tb reads from 66 different RNA-seq datasets were collected to identify AS events. TeaAS supports four methods of retrieval of AS information based on gene ID, gene name, annotation (non-redundant/Kyoto encyclopedia of genes and genomes/gene ontology annotation or chromosomal location) and RNA-seq data. It integrates data pertaining to genome annotation, type of AS event, transcript sequence, and isoforms expression levels from 66 RNA-seq datasets. The AS events resulting from different environmental conditions and that occurring in varied tissue types, and the expression levels of specific transcripts can be clearly identified through this online database. Moreover, it also provides two useful tools, Basic Local Alignment Search Tool and Generic Genome Browser, for sequence alignment and visualization of gene structure. The features of the TeaAS database make it a comprehensive AS bioinformatics platform for researchers, as well as a reference for studying AS events in woody crops. It could also be helpful for revealing the novel biological functions of AS in gene regulation in tea plants. |
| first_indexed | 2024-12-20T03:25:29Z |
| format | Article |
| id | doaj.art-5ec424c27e1c4daeb9aef00061f56c4a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T03:25:29Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-5ec424c27e1c4daeb9aef00061f56c4a2022-12-21T19:55:06ZengBMCBMC Plant Biology1471-22292021-06-012111910.1186/s12870-021-03065-8TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis)Xiaozeng Mi0Yi Yue1Mengsha Tang2Yanlin An3Hui Xie4Dahe Qiao5Zhiyu Ma6Shengrui Liu7Chaoling Wei8State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversitySchool of Information and Computer, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversitySchool of Information and Computer, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityAbstract Alternative splicing (AS) increases the diversity of transcripts and proteins through the selection of different splice sites and plays an important role in the growth, development and stress tolerance of plants. With the release of the reference genome of the tea plant (Camellia sinensis) and the development of transcriptome sequencing, researchers have reported the existence of AS in tea plants. However, there is a lack of a platform, centered on different RNA-seq datasets, that provides comprehensive information on AS. To facilitate access to information on AS and reveal the molecular function of AS in tea plants, we established the first comprehensive AS database for tea plants (TeaAS, http://www.teaas.cn/index.php ). In this study, 3.96 Tb reads from 66 different RNA-seq datasets were collected to identify AS events. TeaAS supports four methods of retrieval of AS information based on gene ID, gene name, annotation (non-redundant/Kyoto encyclopedia of genes and genomes/gene ontology annotation or chromosomal location) and RNA-seq data. It integrates data pertaining to genome annotation, type of AS event, transcript sequence, and isoforms expression levels from 66 RNA-seq datasets. The AS events resulting from different environmental conditions and that occurring in varied tissue types, and the expression levels of specific transcripts can be clearly identified through this online database. Moreover, it also provides two useful tools, Basic Local Alignment Search Tool and Generic Genome Browser, for sequence alignment and visualization of gene structure. The features of the TeaAS database make it a comprehensive AS bioinformatics platform for researchers, as well as a reference for studying AS events in woody crops. It could also be helpful for revealing the novel biological functions of AS in gene regulation in tea plants.https://doi.org/10.1186/s12870-021-03065-8Alternative splicingGenome and transcriptomeTranscriptsIsoformsTea plant |
| spellingShingle | Xiaozeng Mi Yi Yue Mengsha Tang Yanlin An Hui Xie Dahe Qiao Zhiyu Ma Shengrui Liu Chaoling Wei TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) BMC Plant Biology Alternative splicing Genome and transcriptome Transcripts Isoforms Tea plant |
| title | TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) |
| title_full | TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) |
| title_fullStr | TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) |
| title_full_unstemmed | TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) |
| title_short | TeaAS: a comprehensive database for alternative splicing in tea plants (Camellia sinensis) |
| title_sort | teaas a comprehensive database for alternative splicing in tea plants camellia sinensis |
| topic | Alternative splicing Genome and transcriptome Transcripts Isoforms Tea plant |
| url | https://doi.org/10.1186/s12870-021-03065-8 |
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