Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis
Both exposure to antibiotics at birth and delivery via Caesarean section influence the gut bacteriome’s development in infants. Using 16S rRNA and internal transcribed spacer sequencing on the Ion Torrent platform, we employed network analysis to investigate the bacterial and fungal interkingdom rel...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/7/718 |
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| author | Mysore V. Tejesvi Jenni Turunen Sonja Salmi Justus Reunanen Niko Paalanne Terhi Tapiainen |
| author_facet | Mysore V. Tejesvi Jenni Turunen Sonja Salmi Justus Reunanen Niko Paalanne Terhi Tapiainen |
| author_sort | Mysore V. Tejesvi |
| collection | DOAJ |
| description | Both exposure to antibiotics at birth and delivery via Caesarean section influence the gut bacteriome’s development in infants. Using 16S rRNA and internal transcribed spacer sequencing on the Ion Torrent platform, we employed network analysis to investigate the bacterial and fungal interkingdom relationships in the gut microbiome from birth to age 18 months in a prospective cohort study of 140 infants. The gut microbiome at ages six and 18 months revealed distinctive microbial interactions, including both positive and negative associations between bacterial and fungal genera in the gut ecosystem. Perinatal factors, delivery mode and intrapartum antibiotic exposure affected the associations between bacterial and fungal species. In infants exposed and unexposed to perinatal antibiotics, the gut microbiome formed distinct networks for the bacteriome and mycobiome. The fungi <i>Saccharomyces</i>, <i>Trichosporon</i>, <i>Pezoloma</i>, <i>Cystofilobasidium</i>, <i>Rigidoporus</i> and <i>Fomitopsis</i> were strongly associated with exposure to antibiotics at birth. <i>Hyaloscypha</i>, <i>Trichosporon</i>, <i>Fomitopsis</i> and <i>Vishniacozyma</i> were strongly associated with the control group that was not exposed to antibiotics. Five distinct networks were formed according to delivery mode. The present study confirms that bacteria and fungi clearly interact in the infant gut ecosystem. Furthermore, perinatal factors appear to influence the relationships between bacteria and fungi in the developing gut microbiome. |
| first_indexed | 2024-03-11T00:55:41Z |
| format | Article |
| id | doaj.art-5ec5d1f039024221a5ba630e57e62a80 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T00:55:41Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-5ec5d1f039024221a5ba630e57e62a802023-11-18T20:01:23ZengMDPI AGJournal of Fungi2309-608X2023-06-019771810.3390/jof9070718Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network AnalysisMysore V. Tejesvi0Jenni Turunen1Sonja Salmi2Justus Reunanen3Niko Paalanne4Terhi Tapiainen5Research Unit of Clinical Medicine, University of Oulu, 90014 Oulu, FinlandResearch Unit of Clinical Medicine, University of Oulu, 90014 Oulu, FinlandBiocenter Oulu, University of Oulu, 90014 Oulu, FinlandBiocenter Oulu, University of Oulu, 90014 Oulu, FinlandResearch Unit of Clinical Medicine, University of Oulu, 90014 Oulu, FinlandResearch Unit of Clinical Medicine, University of Oulu, 90014 Oulu, FinlandBoth exposure to antibiotics at birth and delivery via Caesarean section influence the gut bacteriome’s development in infants. Using 16S rRNA and internal transcribed spacer sequencing on the Ion Torrent platform, we employed network analysis to investigate the bacterial and fungal interkingdom relationships in the gut microbiome from birth to age 18 months in a prospective cohort study of 140 infants. The gut microbiome at ages six and 18 months revealed distinctive microbial interactions, including both positive and negative associations between bacterial and fungal genera in the gut ecosystem. Perinatal factors, delivery mode and intrapartum antibiotic exposure affected the associations between bacterial and fungal species. In infants exposed and unexposed to perinatal antibiotics, the gut microbiome formed distinct networks for the bacteriome and mycobiome. The fungi <i>Saccharomyces</i>, <i>Trichosporon</i>, <i>Pezoloma</i>, <i>Cystofilobasidium</i>, <i>Rigidoporus</i> and <i>Fomitopsis</i> were strongly associated with exposure to antibiotics at birth. <i>Hyaloscypha</i>, <i>Trichosporon</i>, <i>Fomitopsis</i> and <i>Vishniacozyma</i> were strongly associated with the control group that was not exposed to antibiotics. Five distinct networks were formed according to delivery mode. The present study confirms that bacteria and fungi clearly interact in the infant gut ecosystem. Furthermore, perinatal factors appear to influence the relationships between bacteria and fungi in the developing gut microbiome.https://www.mdpi.com/2309-608X/9/7/718mycobiomebacteriome16S rRNAdelivery modeintrapartum antibioticsnetwork analysis |
| spellingShingle | Mysore V. Tejesvi Jenni Turunen Sonja Salmi Justus Reunanen Niko Paalanne Terhi Tapiainen Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis Journal of Fungi mycobiome bacteriome 16S rRNA delivery mode intrapartum antibiotics network analysis |
| title | Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis |
| title_full | Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis |
| title_fullStr | Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis |
| title_full_unstemmed | Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis |
| title_short | Delivery Mode and Perinatal Antibiotics Influence the Infant Gut Bacteriome and Mycobiome: A Network Analysis |
| title_sort | delivery mode and perinatal antibiotics influence the infant gut bacteriome and mycobiome a network analysis |
| topic | mycobiome bacteriome 16S rRNA delivery mode intrapartum antibiotics network analysis |
| url | https://www.mdpi.com/2309-608X/9/7/718 |
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