dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.
To maintain a particular cell fate, a unique set of genes should be expressed while another set is repressed. One way to repress gene expression is through Polycomb group (PcG) proteins that compact chromatin into a silent configuration. In addition to cell fate maintenance, PcG proteins also mainta...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-09-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5010193?pdf=render |
| _version_ | 1811295224467554304 |
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| author | Hsueh-Tzu Shih Wei-Yu Chen Kwei-Yan Liu Zong-Siou Shih Yi-Jyun Chen Paul-Chen Hsieh Kuan-Lin Kuo Kuo-How Huang Pang-Hung Hsu Ya-Wen Liu Shih-Peng Chan Hsiu-Hsiang Lee Yu-Chen Tsai June-Tai Wu |
| author_facet | Hsueh-Tzu Shih Wei-Yu Chen Kwei-Yan Liu Zong-Siou Shih Yi-Jyun Chen Paul-Chen Hsieh Kuan-Lin Kuo Kuo-How Huang Pang-Hung Hsu Ya-Wen Liu Shih-Peng Chan Hsiu-Hsiang Lee Yu-Chen Tsai June-Tai Wu |
| author_sort | Hsueh-Tzu Shih |
| collection | DOAJ |
| description | To maintain a particular cell fate, a unique set of genes should be expressed while another set is repressed. One way to repress gene expression is through Polycomb group (PcG) proteins that compact chromatin into a silent configuration. In addition to cell fate maintenance, PcG proteins also maintain normal cell physiology, for example cell cycle. In the absence of PcG, ectopic activation of the PcG-repressed genes leads to developmental defects and malignant tumors. Little is known about the molecular nature of ectopic gene expression; especially what differentiates expression of a given gene in the orthotopic tissue (orthotopic expression) and the ectopic expression of the same gene due to PcG mutations. Here we present that ectopic gene expression in PcG mutant cells specifically requires dBRWD3, a negative regulator of HIRA/Yemanuclein (YEM)-mediated histone variant H3.3 deposition. dBRWD3 mutations suppress both the ectopic gene expression and aberrant tissue overgrowth in PcG mutants through a YEM-dependent mechanism. Our findings identified dBRWD3 as a critical regulator that is uniquely required for ectopic gene expression and aberrant tissue overgrowth caused by PcG mutations. |
| first_indexed | 2024-04-13T05:29:23Z |
| format | Article |
| id | doaj.art-5ec658267b334fec96fda5242c7de434 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T05:29:23Z |
| publishDate | 2016-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-5ec658267b334fec96fda5242c7de4342022-12-22T03:00:29ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-09-01129e100626210.1371/journal.pgen.1006262dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.Hsueh-Tzu ShihWei-Yu ChenKwei-Yan LiuZong-Siou ShihYi-Jyun ChenPaul-Chen HsiehKuan-Lin KuoKuo-How HuangPang-Hung HsuYa-Wen LiuShih-Peng ChanHsiu-Hsiang LeeYu-Chen TsaiJune-Tai WuTo maintain a particular cell fate, a unique set of genes should be expressed while another set is repressed. One way to repress gene expression is through Polycomb group (PcG) proteins that compact chromatin into a silent configuration. In addition to cell fate maintenance, PcG proteins also maintain normal cell physiology, for example cell cycle. In the absence of PcG, ectopic activation of the PcG-repressed genes leads to developmental defects and malignant tumors. Little is known about the molecular nature of ectopic gene expression; especially what differentiates expression of a given gene in the orthotopic tissue (orthotopic expression) and the ectopic expression of the same gene due to PcG mutations. Here we present that ectopic gene expression in PcG mutant cells specifically requires dBRWD3, a negative regulator of HIRA/Yemanuclein (YEM)-mediated histone variant H3.3 deposition. dBRWD3 mutations suppress both the ectopic gene expression and aberrant tissue overgrowth in PcG mutants through a YEM-dependent mechanism. Our findings identified dBRWD3 as a critical regulator that is uniquely required for ectopic gene expression and aberrant tissue overgrowth caused by PcG mutations.http://europepmc.org/articles/PMC5010193?pdf=render |
| spellingShingle | Hsueh-Tzu Shih Wei-Yu Chen Kwei-Yan Liu Zong-Siou Shih Yi-Jyun Chen Paul-Chen Hsieh Kuan-Lin Kuo Kuo-How Huang Pang-Hung Hsu Ya-Wen Liu Shih-Peng Chan Hsiu-Hsiang Lee Yu-Chen Tsai June-Tai Wu dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. PLoS Genetics |
| title | dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. |
| title_full | dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. |
| title_fullStr | dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. |
| title_full_unstemmed | dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. |
| title_short | dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations. |
| title_sort | dbrwd3 regulates tissue overgrowth and ectopic gene expression caused by polycomb group mutations |
| url | http://europepmc.org/articles/PMC5010193?pdf=render |
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