Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion
Background: Increased oxidative stress is a hallmark of end-stage renal disease. Hemodialysis (HD) patients lacking glutathione transferase M1 (GSTM1) enzyme activity exhibit enhanced oxidative DNA damage and higher mortality rate than those with active GSTM1 enzyme. To our knowledge, this is the fi...
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MDPI AG
2020-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/6/352 |
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| author | Petar Djuric Sonja Suvakov Tatjana Simic Dragana Markovic Djurdja Jerotic Aleksandar Jankovic Ana Bulatovic Jelena Tosic Dragovic Tatjana Damjanovic Jelena Marinkovic Radomir Naumovic Nada Dimkovic |
| author_facet | Petar Djuric Sonja Suvakov Tatjana Simic Dragana Markovic Djurdja Jerotic Aleksandar Jankovic Ana Bulatovic Jelena Tosic Dragovic Tatjana Damjanovic Jelena Marinkovic Radomir Naumovic Nada Dimkovic |
| author_sort | Petar Djuric |
| collection | DOAJ |
| description | Background: Increased oxidative stress is a hallmark of end-stage renal disease. Hemodialysis (HD) patients lacking glutathione transferase M1 (GSTM1) enzyme activity exhibit enhanced oxidative DNA damage and higher mortality rate than those with active GSTM1 enzyme. To our knowledge, this is the first study to use the vitamin E-bonded membranes (VEM) in patients with homozygous <i>GSTM1</i> gene deletion, and we aimed to determine the effect of VEM on oxidative and inflammatory status in HD patients with homozygous <i>GSTM1</i> gene deletion. Methods: <i>GSTM1</i> genotypes were determined by polymerase chain reaction (PCR) in 170 chronic HD patients. Those with <i>GSTM1-null</i> genotype were randomized and 80 were included in the study. Forty of them were dialyzed for three months with VEM, while the other forty were dialyzed with high-flux same-surface polysulfone dialyzers. Markers of protein and lipid oxidative damage and inflammation (thiol groups, malondialdehyde (MDA), Interleukin-6 (IL-6)), together with plasma antioxidant activity (glutathione peroxidase (GPX), superoxide dismutase (SOD)) were determined. Results: Seventy-five patients finished the study. There were no differences at baseline in markers of protein and lipid oxidative damage, inflammation and plasma antioxidant activity. After three months of therapy, GPX, MDA, and thiol groups increased significantly in both groups, but without statistical significance between groups. SOD and C reactive protein (CRP) did not change significantly during the three-month period. IL-6 increased in the control group, and at the same time, decreased in the VEM group, but without statistical significance. Hemoglobin (Hb) value, red blood cells, erythropoiesis resistance index (ERI), serum ferritin and iron did not change significantly within or between groups. Regarding other laboratory parameters, proteins, albumins, triglycerides, serum phosphorus, serum bicarbonate and Kt/V showed significant improvements within groups but with no significant difference between groups. Conclusions: Our data shows that therapy with VEM over three months had no benefit over standard polysulfone membrane in decreasing by-products of oxidative stress and inflammation in dialysis patients lacking GSTM1 enzyme activity. |
| first_indexed | 2024-03-10T19:33:40Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T19:33:40Z |
| publishDate | 2020-05-01 |
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| record_format | Article |
| series | Toxins |
| spelling | doaj.art-5eca240c1f274f0c9a360fbcee0beed52023-11-20T01:55:16ZengMDPI AGToxins2072-66512020-05-0112635210.3390/toxins12060352Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene DeletionPetar Djuric0Sonja Suvakov1Tatjana Simic2Dragana Markovic3Djurdja Jerotic4Aleksandar Jankovic5Ana Bulatovic6Jelena Tosic Dragovic7Tatjana Damjanovic8Jelena Marinkovic9Radomir Naumovic10Nada Dimkovic11Clinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaInstitute of Medical and Clinical Biochemistry, 11000 Belgrade, SerbiaInstitute of Medical and Clinical Biochemistry, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaInstitute of Medical and Clinical Biochemistry, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaInstitute of Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaClinical Department for Renal Diseases, Zvezdara University Medical Center, 11000 Belgrade, SerbiaBackground: Increased oxidative stress is a hallmark of end-stage renal disease. Hemodialysis (HD) patients lacking glutathione transferase M1 (GSTM1) enzyme activity exhibit enhanced oxidative DNA damage and higher mortality rate than those with active GSTM1 enzyme. To our knowledge, this is the first study to use the vitamin E-bonded membranes (VEM) in patients with homozygous <i>GSTM1</i> gene deletion, and we aimed to determine the effect of VEM on oxidative and inflammatory status in HD patients with homozygous <i>GSTM1</i> gene deletion. Methods: <i>GSTM1</i> genotypes were determined by polymerase chain reaction (PCR) in 170 chronic HD patients. Those with <i>GSTM1-null</i> genotype were randomized and 80 were included in the study. Forty of them were dialyzed for three months with VEM, while the other forty were dialyzed with high-flux same-surface polysulfone dialyzers. Markers of protein and lipid oxidative damage and inflammation (thiol groups, malondialdehyde (MDA), Interleukin-6 (IL-6)), together with plasma antioxidant activity (glutathione peroxidase (GPX), superoxide dismutase (SOD)) were determined. Results: Seventy-five patients finished the study. There were no differences at baseline in markers of protein and lipid oxidative damage, inflammation and plasma antioxidant activity. After three months of therapy, GPX, MDA, and thiol groups increased significantly in both groups, but without statistical significance between groups. SOD and C reactive protein (CRP) did not change significantly during the three-month period. IL-6 increased in the control group, and at the same time, decreased in the VEM group, but without statistical significance. Hemoglobin (Hb) value, red blood cells, erythropoiesis resistance index (ERI), serum ferritin and iron did not change significantly within or between groups. Regarding other laboratory parameters, proteins, albumins, triglycerides, serum phosphorus, serum bicarbonate and Kt/V showed significant improvements within groups but with no significant difference between groups. Conclusions: Our data shows that therapy with VEM over three months had no benefit over standard polysulfone membrane in decreasing by-products of oxidative stress and inflammation in dialysis patients lacking GSTM1 enzyme activity.https://www.mdpi.com/2072-6651/12/6/352hemodialysisoxidative stressinflammationvitamin E-bonded membranes |
| spellingShingle | Petar Djuric Sonja Suvakov Tatjana Simic Dragana Markovic Djurdja Jerotic Aleksandar Jankovic Ana Bulatovic Jelena Tosic Dragovic Tatjana Damjanovic Jelena Marinkovic Radomir Naumovic Nada Dimkovic Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion Toxins hemodialysis oxidative stress inflammation vitamin E-bonded membranes |
| title | Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion |
| title_full | Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion |
| title_fullStr | Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion |
| title_full_unstemmed | Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion |
| title_short | Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion |
| title_sort | vitamin e bonded membranes do not influence markers of oxidative stress in hemodialysis patients with homozygous glutathione transferase m1 gene deletion |
| topic | hemodialysis oxidative stress inflammation vitamin E-bonded membranes |
| url | https://www.mdpi.com/2072-6651/12/6/352 |
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