Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey
Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. Th...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-09-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.2478/acve-2023-0024 |
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| author | Çelik Burçak Aslan Çelik Özgür Yaşar Ayan Adnan Akyildiz Gürkan Kilinç Özlem Orunç Ayan Özge Oktay Ercan Kerem |
| author_facet | Çelik Burçak Aslan Çelik Özgür Yaşar Ayan Adnan Akyildiz Gürkan Kilinç Özlem Orunç Ayan Özge Oktay Ercan Kerem |
| author_sort | Çelik Burçak Aslan |
| collection | DOAJ |
| description | Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis. Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the β-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans. |
| first_indexed | 2024-03-11T20:33:25Z |
| format | Article |
| id | doaj.art-5ecb9c55505f487ca2c68121887f00e4 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-03-11T20:33:25Z |
| publishDate | 2023-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-5ecb9c55505f487ca2c68121887f00e42023-10-02T07:38:58ZengSciendoActa Veterinaria1820-74482023-09-0173331732410.2478/acve-2023-0024Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, TurkeyÇelik Burçak Aslan0Çelik Özgür Yaşar1Ayan Adnan2Akyildiz Gürkan3Kilinç Özlem Orunç4Ayan Özge Oktay5Ercan Kerem61Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey2Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey3Department of Genetics, Faculty of Veterinary Medicine, Van Yüzüncü Yıl University, Van, Turkey4Department of Basic Health Sciences, Faculty of Health Sciences, Marmara University, İstanbul, Turkey5Özalp Vocational School, Van Yüzüncü Yıl University, Van, Turkey6Department of Parasitology, Van Yüzüncü Yıl University, Faculty of Medicine, Van, Turkey7Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey.Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis. Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the β-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans.https://doi.org/10.2478/acve-2023-0024nested pcrsequencephylogeny |
| spellingShingle | Çelik Burçak Aslan Çelik Özgür Yaşar Ayan Adnan Akyildiz Gürkan Kilinç Özlem Orunç Ayan Özge Oktay Ercan Kerem Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey Acta Veterinaria nested pcr sequence phylogeny |
| title | Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey |
| title_full | Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey |
| title_fullStr | Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey |
| title_full_unstemmed | Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey |
| title_short | Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey |
| title_sort | preliminary investigation of the prevalence and genotype distribution of cryptosporidium spp and giardia duodenalis in cats in siirt turkey |
| topic | nested pcr sequence phylogeny |
| url | https://doi.org/10.2478/acve-2023-0024 |
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