Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments
In vitro oxidation is a problem for some herbaceous and woody species and can cause darkening of tissues and consequently death of explants and plants Therefore, this study aimed to assess the effect of activated charcoal on in vitro yam cultivation, aiming at reducing or eliminating explant oxidat...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2022-10-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/61950 |
| _version_ | 1811247372774146048 |
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| author | Denise dos Santos Vila Verde Maria Inês de Souza Mendes Antônio da Silva Souza Jéssica Sales Rabêlo Camila Rodrigues Pinto Leila Vasconcelos Costa Nobre Carlos Alberto da Silva Ledo |
| author_facet | Denise dos Santos Vila Verde Maria Inês de Souza Mendes Antônio da Silva Souza Jéssica Sales Rabêlo Camila Rodrigues Pinto Leila Vasconcelos Costa Nobre Carlos Alberto da Silva Ledo |
| author_sort | Denise dos Santos Vila Verde |
| collection | DOAJ |
| description |
In vitro oxidation is a problem for some herbaceous and woody species and can cause darkening of tissues and consequently death of explants and plants Therefore, this study aimed to assess the effect of activated charcoal on in vitro yam cultivation, aiming at reducing or eliminating explant oxidation and optimizing the growth of the genotypes Dioscorea alata var. purpurea (Roxb.) A. Pouchet and Dioscorea rotundata Poir. Nodal segments of approximately 1 cm, extracted from plants previously grown in vitro, were introduced into test tubes containing 10 mL of 2GGC culture medium, plus 30 g L-1 sucrose, solidified with 2.2 g L-1 Phytagel® and pH adjusted to 5.8 before autoclaving, containing activated charcoal doses of 0, 1, 2, 3 and 4 g L-1. Plants were maintained for 90 days in a growth room, with temperature of 27 ± 1ºC, photon flux density of 30 μmol m-2 s-1 and photoperiod of 16 hours, after which their development variables were evaluated. Activated charcoal, at the concentration of 4 g L-1 considerably promoted the best development of plants, and the species D. alata var. purpurea showed higher means for all variables studied.
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| first_indexed | 2024-04-12T15:07:39Z |
| format | Article |
| id | doaj.art-5ecc48d0e56c429489b3d54b6be5ecee |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-12T15:07:39Z |
| publishDate | 2022-10-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-5ecc48d0e56c429489b3d54b6be5ecee2022-12-22T03:27:52ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2022-10-0144110.4025/actascibiolsci.v44i1.61950Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segmentsDenise dos Santos Vila Verde0Maria Inês de Souza Mendes1Antônio da Silva Souza2Jéssica Sales Rabêlo3Camila Rodrigues Pinto4Leila Vasconcelos Costa Nobre5Carlos Alberto da Silva Ledo6Universidade Estadual de Santa CruzEmpresa Brasileira de Pesquisa AgropecuáriaEmpresa Brasileira de Pesquisa AgropecuáriaUniversidade Federal do Recôncavo da BahiaUniversidade Federal do Recôncavo da BahiaUniversidade Federal do Recôncavo da BahiaEmpresa Brasileira de Pesquisa Agropecuária In vitro oxidation is a problem for some herbaceous and woody species and can cause darkening of tissues and consequently death of explants and plants Therefore, this study aimed to assess the effect of activated charcoal on in vitro yam cultivation, aiming at reducing or eliminating explant oxidation and optimizing the growth of the genotypes Dioscorea alata var. purpurea (Roxb.) A. Pouchet and Dioscorea rotundata Poir. Nodal segments of approximately 1 cm, extracted from plants previously grown in vitro, were introduced into test tubes containing 10 mL of 2GGC culture medium, plus 30 g L-1 sucrose, solidified with 2.2 g L-1 Phytagel® and pH adjusted to 5.8 before autoclaving, containing activated charcoal doses of 0, 1, 2, 3 and 4 g L-1. Plants were maintained for 90 days in a growth room, with temperature of 27 ± 1ºC, photon flux density of 30 μmol m-2 s-1 and photoperiod of 16 hours, after which their development variables were evaluated. Activated charcoal, at the concentration of 4 g L-1 considerably promoted the best development of plants, and the species D. alata var. purpurea showed higher means for all variables studied. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/61950phenolic compounds; antioxidants; tissue culture; in vitro multiplication. |
| spellingShingle | Denise dos Santos Vila Verde Maria Inês de Souza Mendes Antônio da Silva Souza Jéssica Sales Rabêlo Camila Rodrigues Pinto Leila Vasconcelos Costa Nobre Carlos Alberto da Silva Ledo Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments Acta Scientiarum: Biological Sciences phenolic compounds; antioxidants; tissue culture; in vitro multiplication. |
| title | Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments |
| title_full | Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments |
| title_fullStr | Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments |
| title_full_unstemmed | Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments |
| title_short | Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments |
| title_sort | activated charcoal in the control of oxidation and in vitro growth of dioscorea spp nodal segments |
| topic | phenolic compounds; antioxidants; tissue culture; in vitro multiplication. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/61950 |
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