Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation
This study explored the intracellular metabolic variations between 17 strains of Saccharomyces cerevisiae belonging to two different genetic populations: flor and wine yeasts, in the context of alcoholic fermentation. These two populations are closely related as they share the same ecological niche...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1227520/full |
| _version_ | 1797656049742774272 |
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| author | Charlotte Vion Charlotte Vion Mathilde Brambati Mathilde Brambati Grégory Da Costa Tristan Richard Philippe Marullo Philippe Marullo |
| author_facet | Charlotte Vion Charlotte Vion Mathilde Brambati Mathilde Brambati Grégory Da Costa Tristan Richard Philippe Marullo Philippe Marullo |
| author_sort | Charlotte Vion |
| collection | DOAJ |
| description | This study explored the intracellular metabolic variations between 17 strains of Saccharomyces cerevisiae belonging to two different genetic populations: flor and wine yeasts, in the context of alcoholic fermentation. These two populations are closely related as they share the same ecological niche but display distinct genetic characteristics. A protocol was developed for intracellular metabolites extraction and 1H-NMR analysis. This methodology allowed us to identify and quantify 21 intracellular metabolites at two different fermentation steps: the exponential and stationary phases. This work provided evidence of significant differences in the abundance of intracellular metabolites, which are strain- and time-dependent, thus revealing complex interactions. Moreover, the differences in abundance appeared to be correlated with life-history traits such as average cell size and specific glycolytic flux, which revealed unsuspected phenotypic correlations between metabolite load and fermentation activity. |
| first_indexed | 2024-03-11T17:23:40Z |
| format | Article |
| id | doaj.art-5ecfcae813b944578f37b5337293df83 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T17:23:40Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5ecfcae813b944578f37b5337293df832023-10-19T07:16:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12275201227520Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentationCharlotte Vion0Charlotte Vion1Mathilde Brambati2Mathilde Brambati3Grégory Da Costa4Tristan Richard5Philippe Marullo6Philippe Marullo7Biolaffort, Bordeaux, FranceUMR Oenologie 1366, Université de Bordeaux, INRAE, Bordeaux INP, BSA, ISVV, Paris, FranceBiolaffort, Bordeaux, FranceUMR Oenologie 1366, Université de Bordeaux, INRAE, Bordeaux INP, BSA, ISVV, Paris, FranceUMR Oenologie 1366, Université de Bordeaux, INRAE, Bordeaux INP, BSA, ISVV, Paris, FranceUMR Oenologie 1366, Université de Bordeaux, INRAE, Bordeaux INP, BSA, ISVV, Paris, FranceBiolaffort, Bordeaux, FranceUMR Oenologie 1366, Université de Bordeaux, INRAE, Bordeaux INP, BSA, ISVV, Paris, FranceThis study explored the intracellular metabolic variations between 17 strains of Saccharomyces cerevisiae belonging to two different genetic populations: flor and wine yeasts, in the context of alcoholic fermentation. These two populations are closely related as they share the same ecological niche but display distinct genetic characteristics. A protocol was developed for intracellular metabolites extraction and 1H-NMR analysis. This methodology allowed us to identify and quantify 21 intracellular metabolites at two different fermentation steps: the exponential and stationary phases. This work provided evidence of significant differences in the abundance of intracellular metabolites, which are strain- and time-dependent, thus revealing complex interactions. Moreover, the differences in abundance appeared to be correlated with life-history traits such as average cell size and specific glycolytic flux, which revealed unsuspected phenotypic correlations between metabolite load and fermentation activity.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1227520/fullphenotypic trade-offsflor and wine yeastmetabolite load1H-NMRfermentation kinetics |
| spellingShingle | Charlotte Vion Charlotte Vion Mathilde Brambati Mathilde Brambati Grégory Da Costa Tristan Richard Philippe Marullo Philippe Marullo Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation Frontiers in Microbiology phenotypic trade-offs flor and wine yeast metabolite load 1H-NMR fermentation kinetics |
| title | Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| title_full | Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| title_fullStr | Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| title_full_unstemmed | Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| title_short | Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| title_sort | endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation |
| topic | phenotypic trade-offs flor and wine yeast metabolite load 1H-NMR fermentation kinetics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1227520/full |
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