Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.649793/full |
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| author | Heloina Nathalliê Mariano da Silva Caroline Covatti Guilherme Luiz da Rocha Daniela Sayuri Mizobuti Rafael Dias Mâncio Túlio de Almeida Hermes Larissa Akemi Kido Valéria Helena Alves Cagnon Elaine Cristina Leite Pereira Elaine Cristina Leite Pereira Elaine Minatel |
| author_facet | Heloina Nathalliê Mariano da Silva Caroline Covatti Guilherme Luiz da Rocha Daniela Sayuri Mizobuti Rafael Dias Mâncio Túlio de Almeida Hermes Larissa Akemi Kido Valéria Helena Alves Cagnon Elaine Cristina Leite Pereira Elaine Cristina Leite Pereira Elaine Minatel |
| author_sort | Heloina Nathalliê Mariano da Silva |
| collection | DOAJ |
| description | The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD. |
| first_indexed | 2024-12-21T18:42:06Z |
| format | Article |
| id | doaj.art-5ed0933327f44dffa343afe6ec9bc76a |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T18:42:06Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-5ed0933327f44dffa343afe6ec9bc76a2022-12-21T18:53:59ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-04-011210.3389/fphys.2021.649793649793Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx MiceHeloina Nathalliê Mariano da Silva0Caroline Covatti1Guilherme Luiz da Rocha2Daniela Sayuri Mizobuti3Rafael Dias Mâncio4Túlio de Almeida Hermes5Larissa Akemi Kido6Valéria Helena Alves Cagnon7Elaine Cristina Leite Pereira8Elaine Cristina Leite Pereira9Elaine Minatel10Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilFaculty of Ceilândia, University of Brasília (UnB), Brasília, BrazilDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, BrazilThe mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD.https://www.frontiersin.org/articles/10.3389/fphys.2021.649793/fulltempoldystrophic musclesexerciseoxidative stressinflammatory process |
| spellingShingle | Heloina Nathalliê Mariano da Silva Caroline Covatti Guilherme Luiz da Rocha Daniela Sayuri Mizobuti Rafael Dias Mâncio Túlio de Almeida Hermes Larissa Akemi Kido Valéria Helena Alves Cagnon Elaine Cristina Leite Pereira Elaine Cristina Leite Pereira Elaine Minatel Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice Frontiers in Physiology tempol dystrophic muscles exercise oxidative stress inflammatory process |
| title | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
| title_full | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
| title_fullStr | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
| title_full_unstemmed | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
| title_short | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
| title_sort | oxidative stress inflammation and activators of mitochondrial biogenesis tempol targets in the diaphragm muscle of exercise trained mdx mice |
| topic | tempol dystrophic muscles exercise oxidative stress inflammatory process |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.649793/full |
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