Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock
No efficient vaccines exist against African swine fever virus (ASFV), which causes a serious disease in wild boars and domestic pigs that produces great industrial and ecological concerns worldwide. An extensive genetic characterization of the original ASFV stocks used to produce live attenuated vac...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/4/625 |
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| author | Daniel Pérez-Núñez Eva Castillo-Rosa Gonzalo Vigara-Astillero Raquel García-Belmonte Carmina Gallardo Yolanda Revilla |
| author_facet | Daniel Pérez-Núñez Eva Castillo-Rosa Gonzalo Vigara-Astillero Raquel García-Belmonte Carmina Gallardo Yolanda Revilla |
| author_sort | Daniel Pérez-Núñez |
| collection | DOAJ |
| description | No efficient vaccines exist against African swine fever virus (ASFV), which causes a serious disease in wild boars and domestic pigs that produces great industrial and ecological concerns worldwide. An extensive genetic characterization of the original ASFV stocks used to produce live attenuated vaccine (LAV) prototypes is needed for vaccine biosecurity and control. Here, we sequenced for the first time the Arm/07 stock which was obtained from an infected pig during the Armenia outbreak in 2007, using an improved viral dsDNA purification method together with high coverage analysis. There was unexpected viral heterogeneity within the stock, with two genetically distinct ASFV subpopulations. The first, represented by the Arm/07/CBM/c2 clone, displayed high sequence identity to the updated genotype II Georgia 2007/1, whereas the second (exemplified by clone Arm/07/CBM/c4) displayed a hemadsorbing phenotype and grouped within genotype I based on a central region conserved among all members of this group. Intriguingly, Arm/07/CBM/c4 contained a unique EP402R sequence, produced by a single mutation in the N-terminal region. Importantly, Arm/07/CBM/c4 showed in vitro features of attenuated strains regarding innate immune response pathway. Both Arm/07/CBM/c2 and c4 represent well-characterized viral clones, useful for different molecular and virus-host interaction studies, including virulence studies and vaccine development. |
| first_indexed | 2024-03-10T15:20:41Z |
| format | Article |
| id | doaj.art-5ed18e7a62254c65965cd1bd2878366d |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T15:20:41Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-5ed18e7a62254c65965cd1bd2878366d2023-11-20T18:28:17ZengMDPI AGVaccines2076-393X2020-10-018462510.3390/vaccines8040625Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 StockDaniel Pérez-Núñez0Eva Castillo-Rosa1Gonzalo Vigara-Astillero2Raquel García-Belmonte3Carmina Gallardo4Yolanda Revilla5Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Microbes in Health and Welfare Department, c/ Nicolás Cabrera, 1, 28049 Madrid, SpainCentro de Biología Molecular Severo Ochoa, CSIC-UAM, Genomics and NGS Core Facility, c/ Nicolás Cabrera, 1, 28049 Madrid, SpainCentro de Biología Molecular Severo Ochoa, CSIC-UAM, Microbes in Health and Welfare Department, c/ Nicolás Cabrera, 1, 28049 Madrid, SpainCentro de Biología Molecular Severo Ochoa, CSIC-UAM, Microbes in Health and Welfare Department, c/ Nicolás Cabrera, 1, 28049 Madrid, SpainEuropean Union Reference Laboratory for African Swine Fever (EURL), INIA-CISA, Valdeolmos, 28049 Madrid, SpainCentro de Biología Molecular Severo Ochoa, CSIC-UAM, Microbes in Health and Welfare Department, c/ Nicolás Cabrera, 1, 28049 Madrid, SpainNo efficient vaccines exist against African swine fever virus (ASFV), which causes a serious disease in wild boars and domestic pigs that produces great industrial and ecological concerns worldwide. An extensive genetic characterization of the original ASFV stocks used to produce live attenuated vaccine (LAV) prototypes is needed for vaccine biosecurity and control. Here, we sequenced for the first time the Arm/07 stock which was obtained from an infected pig during the Armenia outbreak in 2007, using an improved viral dsDNA purification method together with high coverage analysis. There was unexpected viral heterogeneity within the stock, with two genetically distinct ASFV subpopulations. The first, represented by the Arm/07/CBM/c2 clone, displayed high sequence identity to the updated genotype II Georgia 2007/1, whereas the second (exemplified by clone Arm/07/CBM/c4) displayed a hemadsorbing phenotype and grouped within genotype I based on a central region conserved among all members of this group. Intriguingly, Arm/07/CBM/c4 contained a unique EP402R sequence, produced by a single mutation in the N-terminal region. Importantly, Arm/07/CBM/c4 showed in vitro features of attenuated strains regarding innate immune response pathway. Both Arm/07/CBM/c2 and c4 represent well-characterized viral clones, useful for different molecular and virus-host interaction studies, including virulence studies and vaccine development.https://www.mdpi.com/2076-393X/8/4/625ASFVLAVsArm/07 stockArm/07/CBM/c2Arm/07/CBM/c4viral DNA purification |
| spellingShingle | Daniel Pérez-Núñez Eva Castillo-Rosa Gonzalo Vigara-Astillero Raquel García-Belmonte Carmina Gallardo Yolanda Revilla Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock Vaccines ASFV LAVs Arm/07 stock Arm/07/CBM/c2 Arm/07/CBM/c4 viral DNA purification |
| title | Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock |
| title_full | Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock |
| title_fullStr | Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock |
| title_full_unstemmed | Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock |
| title_short | Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock |
| title_sort | identification and isolation of two different subpopulations within african swine fever virus arm 07 stock |
| topic | ASFV LAVs Arm/07 stock Arm/07/CBM/c2 Arm/07/CBM/c4 viral DNA purification |
| url | https://www.mdpi.com/2076-393X/8/4/625 |
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