Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila
ABSTRACT Mycobacterium abscessus, an intracellular nontuberculous mycobacterium, is considered the most pathogenic species among the group of rapidly growing mycobacteria. The resistance of M. abscessus to the host innate response contributes to its pathogenicity in addition to several virulence fac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00777-23 |
| _version_ | 1797741762397077504 |
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| author | Hamadoun Touré Nicolas Durand Isabelle Guénal Jean-Louis Herrmann Fabienne Girard-Misguich Sébastien Szuplewski |
| author_facet | Hamadoun Touré Nicolas Durand Isabelle Guénal Jean-Louis Herrmann Fabienne Girard-Misguich Sébastien Szuplewski |
| author_sort | Hamadoun Touré |
| collection | DOAJ |
| description | ABSTRACT Mycobacterium abscessus, an intracellular nontuberculous mycobacterium, is considered the most pathogenic species among the group of rapidly growing mycobacteria. The resistance of M. abscessus to the host innate response contributes to its pathogenicity in addition to several virulence factors. We have recently shown in Drosophila that antimicrobial peptides (AMPs), whose production is induced by M. abscessus, are unable to control mycobacterial infection. This could be due to their inability to kill mycobacteria and/or the hidden location of the pathogen in phagocytic cells. Here, we demonstrate that the rapid internalization of M. abscessus by Drosophila macrophages allows it to escape the AMP-mediated humoral response. By depleting phagocytes in AMP-deficient flies, we found that several AMPs were required for the control of extracellular M. abscessus. This was confirmed in the Tep4 opsonin-deficient flies, which we show can better control M. abscessus growth and have increased survival through overproduction of some AMPs, including Defensin. Furthermore, Defensin alone was sufficient to kill extracellular M. abscessus both in vitro and in vivo and control its infection. Collectively, our data support that Tep4-mediated opsonization of M. abscessus allows its escape and resistance toward the Defensin bactericidal action in Drosophila. IMPORTANCE Mycobacterium abscessus, an opportunistic pathogen in cystic fibrosis patients, is the most pathogenic species among the fast-growing mycobacteria. How M. abscessus resists the host innate response before establishing an infection remains unclear. Using Drosophila, we have recently demonstrated that M. abscessus resists the host innate response by surviving the cytotoxic lysis of the infected phagocytes and the induced antimicrobial peptides (AMPs), including Defensin. In this work, we demonstrate that M. abscessus resists the latter response by being rapidly internalized by Drosophila phagocytes. Indeed, by combining in vivo and in vitro approaches, we show that Defensin is able to control extracellular M. abscessus infection through a direct bactericidal action. In conclusion, we report that M. abscessus escapes the host AMP-mediated humoral response by taking advantage of its internalization by the phagocytes. |
| first_indexed | 2024-03-12T14:31:20Z |
| format | Article |
| id | doaj.art-5ed4f8f7305747ae95e9ac6b765b2b57 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-12T14:31:20Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-5ed4f8f7305747ae95e9ac6b765b2b572023-08-17T13:04:15ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-08-0111410.1128/spectrum.00777-23Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in DrosophilaHamadoun Touré0Nicolas Durand1Isabelle Guénal2Jean-Louis Herrmann3Fabienne Girard-Misguich4Sébastien Szuplewski5Université Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny-Le-Bretonneux, FranceUniversité Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny-Le-Bretonneux, FranceUniversité Paris-Saclay, UVSQ, LGBC, Versailles, FranceUniversité Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny-Le-Bretonneux, FranceUniversité Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny-Le-Bretonneux, FranceUniversité Paris-Saclay, UVSQ, LGBC, Versailles, FranceABSTRACT Mycobacterium abscessus, an intracellular nontuberculous mycobacterium, is considered the most pathogenic species among the group of rapidly growing mycobacteria. The resistance of M. abscessus to the host innate response contributes to its pathogenicity in addition to several virulence factors. We have recently shown in Drosophila that antimicrobial peptides (AMPs), whose production is induced by M. abscessus, are unable to control mycobacterial infection. This could be due to their inability to kill mycobacteria and/or the hidden location of the pathogen in phagocytic cells. Here, we demonstrate that the rapid internalization of M. abscessus by Drosophila macrophages allows it to escape the AMP-mediated humoral response. By depleting phagocytes in AMP-deficient flies, we found that several AMPs were required for the control of extracellular M. abscessus. This was confirmed in the Tep4 opsonin-deficient flies, which we show can better control M. abscessus growth and have increased survival through overproduction of some AMPs, including Defensin. Furthermore, Defensin alone was sufficient to kill extracellular M. abscessus both in vitro and in vivo and control its infection. Collectively, our data support that Tep4-mediated opsonization of M. abscessus allows its escape and resistance toward the Defensin bactericidal action in Drosophila. IMPORTANCE Mycobacterium abscessus, an opportunistic pathogen in cystic fibrosis patients, is the most pathogenic species among the fast-growing mycobacteria. How M. abscessus resists the host innate response before establishing an infection remains unclear. Using Drosophila, we have recently demonstrated that M. abscessus resists the host innate response by surviving the cytotoxic lysis of the infected phagocytes and the induced antimicrobial peptides (AMPs), including Defensin. In this work, we demonstrate that M. abscessus resists the latter response by being rapidly internalized by Drosophila phagocytes. Indeed, by combining in vivo and in vitro approaches, we show that Defensin is able to control extracellular M. abscessus infection through a direct bactericidal action. In conclusion, we report that M. abscessus escapes the host AMP-mediated humoral response by taking advantage of its internalization by the phagocytes.https://journals.asm.org/doi/10.1128/spectrum.00777-23Mycobacterium abscessusphagocytesopsonizationDefensinDrosophila |
| spellingShingle | Hamadoun Touré Nicolas Durand Isabelle Guénal Jean-Louis Herrmann Fabienne Girard-Misguich Sébastien Szuplewski Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila Microbiology Spectrum Mycobacterium abscessus phagocytes opsonization Defensin Drosophila |
| title | Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila |
| title_full | Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila |
| title_fullStr | Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila |
| title_full_unstemmed | Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila |
| title_short | Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila |
| title_sort | mycobacterium abscessus opsonization allows an escape from the defensin bactericidal action in drosophila |
| topic | Mycobacterium abscessus phagocytes opsonization Defensin Drosophila |
| url | https://journals.asm.org/doi/10.1128/spectrum.00777-23 |
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