Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages
Insights into the modes of action (MoAs) of xenobiotics are of utmost importance for the definition of adverse outcome pathways (AOPs), which are essential for a mechanism-based risk assessment. A well-established strategy to reveal MoAs of xenobiotics is the use of omics. However, often an even mor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/24/9371 |
| _version_ | 1797545236724973568 |
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| author | Isabel Karkossa Stefanie Raps Martin von Bergen Kristin Schubert |
| author_facet | Isabel Karkossa Stefanie Raps Martin von Bergen Kristin Schubert |
| author_sort | Isabel Karkossa |
| collection | DOAJ |
| description | Insights into the modes of action (MoAs) of xenobiotics are of utmost importance for the definition of adverse outcome pathways (AOPs), which are essential for a mechanism-based risk assessment. A well-established strategy to reveal MoAs of xenobiotics is the use of omics. However, often an even more comprehensive approach is needed, which can be achieved using multi-omics. Since the immune system plays a central role in the defense against foreign substances and pathogens, with the innate immune system building a first barrier, we systematically reviewed multi-omics studies investigating the effects of xenobiotics on macrophages. Surprisingly, only nine publications were identified, combining proteomics with transcriptomics or metabolomics. We summarized pathways and single proteins, transcripts, or metabolites, which were described to be affected upon treatment with xenobiotics in the reviewed studies, thus revealing a broad range of effects. In summary, we show that macrophages are a relevant model system to investigate the toxicological effects induced by xenobiotics. Furthermore, the multi-omics approaches led to a more comprehensive overview compared to only one omics layer with slight advantages for combinations that complement each other directly, e.g., proteome and metabolome. |
| first_indexed | 2024-03-10T14:12:38Z |
| format | Article |
| id | doaj.art-5ed5a3d07d704053a9962e2d543214e9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:12:38Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5ed5a3d07d704053a9962e2d543214e92023-11-21T00:01:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012124937110.3390/ijms21249371Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in MacrophagesIsabel Karkossa0Stefanie Raps1Martin von Bergen2Kristin Schubert3Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology, Helmholtz-Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology, Helmholtz-Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology, Helmholtz-Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyInsights into the modes of action (MoAs) of xenobiotics are of utmost importance for the definition of adverse outcome pathways (AOPs), which are essential for a mechanism-based risk assessment. A well-established strategy to reveal MoAs of xenobiotics is the use of omics. However, often an even more comprehensive approach is needed, which can be achieved using multi-omics. Since the immune system plays a central role in the defense against foreign substances and pathogens, with the innate immune system building a first barrier, we systematically reviewed multi-omics studies investigating the effects of xenobiotics on macrophages. Surprisingly, only nine publications were identified, combining proteomics with transcriptomics or metabolomics. We summarized pathways and single proteins, transcripts, or metabolites, which were described to be affected upon treatment with xenobiotics in the reviewed studies, thus revealing a broad range of effects. In summary, we show that macrophages are a relevant model system to investigate the toxicological effects induced by xenobiotics. Furthermore, the multi-omics approaches led to a more comprehensive overview compared to only one omics layer with slight advantages for combinations that complement each other directly, e.g., proteome and metabolome.https://www.mdpi.com/1422-0067/21/24/9371multi-omicsmacrophagestoxicologyxenobiotics |
| spellingShingle | Isabel Karkossa Stefanie Raps Martin von Bergen Kristin Schubert Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages International Journal of Molecular Sciences multi-omics macrophages toxicology xenobiotics |
| title | Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages |
| title_full | Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages |
| title_fullStr | Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages |
| title_full_unstemmed | Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages |
| title_short | Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages |
| title_sort | systematic review of multi omics approaches to investigate toxicological effects in macrophages |
| topic | multi-omics macrophages toxicology xenobiotics |
| url | https://www.mdpi.com/1422-0067/21/24/9371 |
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