Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections
Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/6/667 |
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| author | Pavel Rudenko Yuriy Vatnikov Nadezhda Sachivkina Andrei Rudenko Evgeny Kulikov Vladimir Lutsay Elena Notina Irina Bykova Aleksander Petrov Stanislav Drukovskiy Ifarajimi Rapheal Olabode |
| author_facet | Pavel Rudenko Yuriy Vatnikov Nadezhda Sachivkina Andrei Rudenko Evgeny Kulikov Vladimir Lutsay Elena Notina Irina Bykova Aleksander Petrov Stanislav Drukovskiy Ifarajimi Rapheal Olabode |
| author_sort | Pavel Rudenko |
| collection | DOAJ |
| description | Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting <i>L. acidophilus No. 24</i>, <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 5</i>, <i>L. rhamnosus No. 20,</i> and <i>L. rhamnosus No. 26</i>, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the <i>B. adolescentis No. 23</i> strain of the <i>Bifidobacterium</i> genus, as well as the <i>L. plantarum No. 8</i>, <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 6</i>, <i>L. rhamnosus No. 26</i>, <i>L. acidophilus No. 12,</i> and <i>L. acidophilus No. 24</i> strains of the <i>Lactobacillus</i> genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 26,</i> and <i>L. acidophilus No. 24</i>. |
| first_indexed | 2024-03-10T10:54:35Z |
| format | Article |
| id | doaj.art-5ed95ba131574b74a6598057385c6f3d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T10:54:35Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-5ed95ba131574b74a6598057385c6f3d2023-11-21T21:56:33ZengMDPI AGPathogens2076-08172021-05-0110666710.3390/pathogens10060667Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical InfectionsPavel Rudenko0Yuriy Vatnikov1Nadezhda Sachivkina2Andrei Rudenko3Evgeny Kulikov4Vladimir Lutsay5Elena Notina6Irina Bykova7Aleksander Petrov8Stanislav Drukovskiy9Ifarajimi Rapheal Olabode10Biological Testing Laboratory, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (BIBCh RAS), 142290 Pushchino, RussiaDepartment of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology and Virology, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Moscow State University of Food Production, 125080 Moscow, RussiaDepartment of Microbiology and Virology, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Moscow State University of Food Production, 125080 Moscow, RussiaDepartment of Foreign Languages, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Foreign Languages, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDespite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting <i>L. acidophilus No. 24</i>, <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 5</i>, <i>L. rhamnosus No. 20,</i> and <i>L. rhamnosus No. 26</i>, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the <i>B. adolescentis No. 23</i> strain of the <i>Bifidobacterium</i> genus, as well as the <i>L. plantarum No. 8</i>, <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 6</i>, <i>L. rhamnosus No. 26</i>, <i>L. acidophilus No. 12,</i> and <i>L. acidophilus No. 24</i> strains of the <i>Lactobacillus</i> genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are <i>L. plantarum “Victoria” No. 22</i>, <i>L. rhamnosus No. 26,</i> and <i>L. acidophilus No. 24</i>.https://www.mdpi.com/2076-0817/10/6/667microbiotabiotopeprobioticsantibioticsadhesionantagonism |
| spellingShingle | Pavel Rudenko Yuriy Vatnikov Nadezhda Sachivkina Andrei Rudenko Evgeny Kulikov Vladimir Lutsay Elena Notina Irina Bykova Aleksander Petrov Stanislav Drukovskiy Ifarajimi Rapheal Olabode Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections Pathogens microbiota biotope probiotics antibiotics adhesion antagonism |
| title | Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections |
| title_full | Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections |
| title_fullStr | Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections |
| title_full_unstemmed | Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections |
| title_short | Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections |
| title_sort | search for promising strains of probiotic microbiota isolated from different biotopes of healthy cats for use in the control of surgical infections |
| topic | microbiota biotope probiotics antibiotics adhesion antagonism |
| url | https://www.mdpi.com/2076-0817/10/6/667 |
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