Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes

The objective of this study was to investigate the use of citrus fiber as a carrier of blackberry juice polyphenols. For that purpose, freeze-dried complexes with blackberry juice and different amounts of citrus fiber (1%, 2% and 4%) were prepared. Complexes were evaluated spectrophotometrically for...

Full description

Bibliographic Details
Main Authors: Ivana Buljeta, Anita Pichler, Josip Šimunović, Mirela Kopjar
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/15/4400
_version_ 1797525314270658560
author Ivana Buljeta
Anita Pichler
Josip Šimunović
Mirela Kopjar
author_facet Ivana Buljeta
Anita Pichler
Josip Šimunović
Mirela Kopjar
author_sort Ivana Buljeta
collection DOAJ
description The objective of this study was to investigate the use of citrus fiber as a carrier of blackberry juice polyphenols. For that purpose, freeze-dried complexes with blackberry juice and different amounts of citrus fiber (1%, 2% and 4%) were prepared. Complexes were evaluated spectrophotometrically for total polyphenols, proanthocyanidins and antioxidant activity. Analyses of individual polyphenols were performed using high-performance liquid chromatography. IR spectra were recorded to confirm encapsulation. All analyses were performed after preparation and after eight months of storage, in order to examine the stability of formed complexes. The obtained results indicated that increasing the amount of fiber led to a decrease in the concentration of polyphenols and the antioxidant activity of complexes. Cyanidin 3-glucoside was the prevalent anthocyanin in complexes (138.32–246.45 mg/100 g), while cyanidin 3-dioxalylglucoside was present at lower concentrations (22.19–31.45 mg/100 g). The other identified and quantified polyphenols were hesperidin (from citrus fiber), ellagic acid and quercetin (1317.59–1571.65 mg/100 g, 31.94–50.11 mg/100 g and 20.11–33.77 mg/100 g, respectively). Degradation of polyphenols occurred during storage. Results obtained in this study confirmed that citrus fiber could be used for the formulation of novel bioactive additives. Such additives could enhance the antioxidant potential of products to which they are added, such as baked goods, dairy, or fruit products.
first_indexed 2024-03-10T09:11:05Z
format Article
id doaj.art-5ed9f88833824231bbe5cb4c976da916
institution Directory Open Access Journal
issn 1420-3049
language English
last_indexed 2024-03-10T09:11:05Z
publishDate 2021-07-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj.art-5ed9f88833824231bbe5cb4c976da9162023-11-22T05:57:33ZengMDPI AGMolecules1420-30492021-07-012615440010.3390/molecules26154400Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice ComplexesIvana Buljeta0Anita Pichler1Josip Šimunović2Mirela Kopjar3Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaDepartment of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USAFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaThe objective of this study was to investigate the use of citrus fiber as a carrier of blackberry juice polyphenols. For that purpose, freeze-dried complexes with blackberry juice and different amounts of citrus fiber (1%, 2% and 4%) were prepared. Complexes were evaluated spectrophotometrically for total polyphenols, proanthocyanidins and antioxidant activity. Analyses of individual polyphenols were performed using high-performance liquid chromatography. IR spectra were recorded to confirm encapsulation. All analyses were performed after preparation and after eight months of storage, in order to examine the stability of formed complexes. The obtained results indicated that increasing the amount of fiber led to a decrease in the concentration of polyphenols and the antioxidant activity of complexes. Cyanidin 3-glucoside was the prevalent anthocyanin in complexes (138.32–246.45 mg/100 g), while cyanidin 3-dioxalylglucoside was present at lower concentrations (22.19–31.45 mg/100 g). The other identified and quantified polyphenols were hesperidin (from citrus fiber), ellagic acid and quercetin (1317.59–1571.65 mg/100 g, 31.94–50.11 mg/100 g and 20.11–33.77 mg/100 g, respectively). Degradation of polyphenols occurred during storage. Results obtained in this study confirmed that citrus fiber could be used for the formulation of novel bioactive additives. Such additives could enhance the antioxidant potential of products to which they are added, such as baked goods, dairy, or fruit products.https://www.mdpi.com/1420-3049/26/15/4400citrus fibersblackberry juiceantioxidantspolyphenols profile
spellingShingle Ivana Buljeta
Anita Pichler
Josip Šimunović
Mirela Kopjar
Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
Molecules
citrus fibers
blackberry juice
antioxidants
polyphenols profile
title Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
title_full Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
title_fullStr Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
title_full_unstemmed Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
title_short Polyphenols and Antioxidant Activity of Citrus Fiber/Blackberry Juice Complexes
title_sort polyphenols and antioxidant activity of citrus fiber blackberry juice complexes
topic citrus fibers
blackberry juice
antioxidants
polyphenols profile
url https://www.mdpi.com/1420-3049/26/15/4400
work_keys_str_mv AT ivanabuljeta polyphenolsandantioxidantactivityofcitrusfiberblackberryjuicecomplexes
AT anitapichler polyphenolsandantioxidantactivityofcitrusfiberblackberryjuicecomplexes
AT josipsimunovic polyphenolsandantioxidantactivityofcitrusfiberblackberryjuicecomplexes
AT mirelakopjar polyphenolsandantioxidantactivityofcitrusfiberblackberryjuicecomplexes