The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
Abstract Background Gut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observable differences in molecular and clinical characteristics between patients with right- and left-sided colon cancer, differences in their gut microbiomes have...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-022-02382-y |
| _version_ | 1811235666537742336 |
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| author | Daisuke Suga Hiroki Mizutani Shunsuke Fukui Mayu Kobayashi Yasuaki Shimada Yuuichi Nakazawa Yuuki Nishiura Yuuya Kawasaki Isao Moritani Yutaka Yamanaka Hidekazu Inoue Eiki Ojima Yasuhiko Mohri Hayato Nakagawa Kaoru Dohi Kei Takaba Hideo Wada Katsuya Shiraki |
| author_facet | Daisuke Suga Hiroki Mizutani Shunsuke Fukui Mayu Kobayashi Yasuaki Shimada Yuuichi Nakazawa Yuuki Nishiura Yuuya Kawasaki Isao Moritani Yutaka Yamanaka Hidekazu Inoue Eiki Ojima Yasuhiko Mohri Hayato Nakagawa Kaoru Dohi Kei Takaba Hideo Wada Katsuya Shiraki |
| author_sort | Daisuke Suga |
| collection | DOAJ |
| description | Abstract Background Gut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observable differences in molecular and clinical characteristics between patients with right- and left-sided colon cancer, differences in their gut microbiomes have not been thoroughly investigated. Furthermore, subsequent changes in microbiota status after partial colectomy remain unknown. We examined the human gut microbiota composition to determine its relationship with colon cancer and partial colon resection according to location. Methods Stool samples from forty-one subjects (10 in the control group, 10 in the right-sided colon cancer [RCC] group, 6 in the sigmoid colon cancer [SCC] group, 9 in the right colon resection [RCR] group and 6 in the sigmoid colon resection [SCR] group) were collected, and DNA was extracted. After terminal restriction fragment length polymorphism (T-RFLP) analysis, the samples were subjected to 16S rRNA gene amplicon sequencing, and the metabolic function of the microbiota was predicted using PICRUSt2. Results T-RFLP analysis showed a reduced ratio of clostridial cluster XIVa in the SCC patients and clostridial cluster IX in the RCC patients, although these changes were not evident in the RCR or SCR patients. 16S rRNA gene amplicon sequencing demonstrated that the diversity of the gut microbiota in the RCC group was higher than that in the control group, and the diversity in the SCR group was significantly higher than that in the RCR group. Principal coordinate analysis (PCoA) revealed significant differences according to the group. Analyses of the microbiota revealed that Firmicutes was significantly dominant in the RCC group and that the SCC group had a higher abundance of Verrucomicrobia. At the genus level, linear discriminant analysis effect size (LEfSe) revealed several bacteria, such as Ruminococcaceae, Streptococcaceae, Clostridiaceae, Gemellaceae, and Desulfovibrio, in the RCC group and several oral microbiomes in the SCC group. Metabolic function prediction revealed that cholesterol transport- and metabolism-related enzymes were specifically upregulated in the RCC group and that cobalamin metabolism-related enzymes were downregulated in the SCC group. Conclusion Gut microbial properties differ between RCC and SCC patients and between right hemicolectomy and sigmoidectomy patients and may contribute to clinical manifestations. |
| first_indexed | 2024-04-12T11:55:54Z |
| format | Article |
| id | doaj.art-5edae0560d5b458b9887178703df1910 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-12T11:55:54Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-5edae0560d5b458b9887178703df19102022-12-22T03:34:00ZengBMCBMC Gastroenterology1471-230X2022-06-0122111310.1186/s12876-022-02382-yThe gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencingDaisuke Suga0Hiroki Mizutani1Shunsuke Fukui2Mayu Kobayashi3Yasuaki Shimada4Yuuichi Nakazawa5Yuuki Nishiura6Yuuya Kawasaki7Isao Moritani8Yutaka Yamanaka9Hidekazu Inoue10Eiki Ojima11Yasuhiko Mohri12Hayato Nakagawa13Kaoru Dohi14Kei Takaba15Hideo Wada16Katsuya Shiraki17Department of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterDepartment of Surgery, Mie General Medical CenterDepartment of Surgery, Mie General Medical CenterDepartment of Gastroenterology, Mie University Graduate School of MedicineDepartment of Cardiology and Nephrology, Mie University Graduate School of MedicineDepartment of Research Center, Mie General Medical CenterDepartment of Research Center, Mie General Medical CenterDepartment of Gastroenterology, Mie General Medical CenterAbstract Background Gut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observable differences in molecular and clinical characteristics between patients with right- and left-sided colon cancer, differences in their gut microbiomes have not been thoroughly investigated. Furthermore, subsequent changes in microbiota status after partial colectomy remain unknown. We examined the human gut microbiota composition to determine its relationship with colon cancer and partial colon resection according to location. Methods Stool samples from forty-one subjects (10 in the control group, 10 in the right-sided colon cancer [RCC] group, 6 in the sigmoid colon cancer [SCC] group, 9 in the right colon resection [RCR] group and 6 in the sigmoid colon resection [SCR] group) were collected, and DNA was extracted. After terminal restriction fragment length polymorphism (T-RFLP) analysis, the samples were subjected to 16S rRNA gene amplicon sequencing, and the metabolic function of the microbiota was predicted using PICRUSt2. Results T-RFLP analysis showed a reduced ratio of clostridial cluster XIVa in the SCC patients and clostridial cluster IX in the RCC patients, although these changes were not evident in the RCR or SCR patients. 16S rRNA gene amplicon sequencing demonstrated that the diversity of the gut microbiota in the RCC group was higher than that in the control group, and the diversity in the SCR group was significantly higher than that in the RCR group. Principal coordinate analysis (PCoA) revealed significant differences according to the group. Analyses of the microbiota revealed that Firmicutes was significantly dominant in the RCC group and that the SCC group had a higher abundance of Verrucomicrobia. At the genus level, linear discriminant analysis effect size (LEfSe) revealed several bacteria, such as Ruminococcaceae, Streptococcaceae, Clostridiaceae, Gemellaceae, and Desulfovibrio, in the RCC group and several oral microbiomes in the SCC group. Metabolic function prediction revealed that cholesterol transport- and metabolism-related enzymes were specifically upregulated in the RCC group and that cobalamin metabolism-related enzymes were downregulated in the SCC group. Conclusion Gut microbial properties differ between RCC and SCC patients and between right hemicolectomy and sigmoidectomy patients and may contribute to clinical manifestations.https://doi.org/10.1186/s12876-022-02382-yColon cancerMicrobiotaT-RFLP16S rRNA gene amplicon sequencing |
| spellingShingle | Daisuke Suga Hiroki Mizutani Shunsuke Fukui Mayu Kobayashi Yasuaki Shimada Yuuichi Nakazawa Yuuki Nishiura Yuuya Kawasaki Isao Moritani Yutaka Yamanaka Hidekazu Inoue Eiki Ojima Yasuhiko Mohri Hayato Nakagawa Kaoru Dohi Kei Takaba Hideo Wada Katsuya Shiraki The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing BMC Gastroenterology Colon cancer Microbiota T-RFLP 16S rRNA gene amplicon sequencing |
| title | The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing |
| title_full | The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing |
| title_fullStr | The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing |
| title_full_unstemmed | The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing |
| title_short | The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing |
| title_sort | gut microbiota composition in patients with right and left sided colorectal cancer and after curative colectomy as analyzed by 16s rrna gene amplicon sequencing |
| topic | Colon cancer Microbiota T-RFLP 16S rRNA gene amplicon sequencing |
| url | https://doi.org/10.1186/s12876-022-02382-y |
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