The nasal microbiota in health and disease: variation within and between subjects
Chronic rhinosinusitis (CRS) affects approximately 5% of the adult population in Western societies and severely reduces the patient’s quality of life. The role of bacteria in the pathogenesis of this condition has yet not been established with certainty. However, recent reports of bacterial and fung...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00134/full |
| _version_ | 1811257697541029888 |
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| author | Kristi eBiswas Michael eHoggard Ravi eJain Michael W. Taylor Richard eDouglas |
| author_facet | Kristi eBiswas Michael eHoggard Ravi eJain Michael W. Taylor Richard eDouglas |
| author_sort | Kristi eBiswas |
| collection | DOAJ |
| description | Chronic rhinosinusitis (CRS) affects approximately 5% of the adult population in Western societies and severely reduces the patient’s quality of life. The role of bacteria in the pathogenesis of this condition has yet not been established with certainty. However, recent reports of bacterial and fungal biofilms in CRS highlight a potential role for these microorganisms. In this study, 16S rRNA gene-targeted amplicon pyrosequencing and qPCR were used to determine the composition and abundance, respectively, of the sinus microbiota within 9 patients with CRS and 6 healthy individuals. Within-patient variability was also investigated by sampling from anterior nares, inferior turbinate and middle meatus on each side of the sinuses. Our results indicate that more of the variation in bacterial composition can be explained by inter-personal differences, rather than sampling location or even disease status. In addition, bacterial community diversity was significantly lower in CRS samples compared to those from healthy subjects, whereas bacterial load was not associated with disease status. Although members of the genera Corynebacterium and Staphylococcus were prevalent in the majority of samples (including healthy subjects), the large amount of variation observed between individuals, particularly within the CRS cohort, suggests that an imbalance or dysbiosis in community structure could be the driving force behind the disease. Ultimately, understanding the causes of variation within the sinus microbiota may lead to more personalised treatment options for CRS. |
| first_indexed | 2024-04-12T18:01:29Z |
| format | Article |
| id | doaj.art-5edb0b13657e4d4181eba5a38b725818 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T18:01:29Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5edb0b13657e4d4181eba5a38b7258182022-12-22T03:22:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-03-01610.3389/fmicb.2015.00134125604The nasal microbiota in health and disease: variation within and between subjectsKristi eBiswas0Michael eHoggard1Ravi eJain2Michael W. Taylor3Richard eDouglas4The University of AucklandThe University of AucklandThe University of AucklandThe University of AucklandThe University of AucklandChronic rhinosinusitis (CRS) affects approximately 5% of the adult population in Western societies and severely reduces the patient’s quality of life. The role of bacteria in the pathogenesis of this condition has yet not been established with certainty. However, recent reports of bacterial and fungal biofilms in CRS highlight a potential role for these microorganisms. In this study, 16S rRNA gene-targeted amplicon pyrosequencing and qPCR were used to determine the composition and abundance, respectively, of the sinus microbiota within 9 patients with CRS and 6 healthy individuals. Within-patient variability was also investigated by sampling from anterior nares, inferior turbinate and middle meatus on each side of the sinuses. Our results indicate that more of the variation in bacterial composition can be explained by inter-personal differences, rather than sampling location or even disease status. In addition, bacterial community diversity was significantly lower in CRS samples compared to those from healthy subjects, whereas bacterial load was not associated with disease status. Although members of the genera Corynebacterium and Staphylococcus were prevalent in the majority of samples (including healthy subjects), the large amount of variation observed between individuals, particularly within the CRS cohort, suggests that an imbalance or dysbiosis in community structure could be the driving force behind the disease. Ultimately, understanding the causes of variation within the sinus microbiota may lead to more personalised treatment options for CRS.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00134/full16S rRNA genebacterial diversityBacterial communitiesBacterial abundanceChronic rhinosinusitis (CRS) |
| spellingShingle | Kristi eBiswas Michael eHoggard Ravi eJain Michael W. Taylor Richard eDouglas The nasal microbiota in health and disease: variation within and between subjects Frontiers in Microbiology 16S rRNA gene bacterial diversity Bacterial communities Bacterial abundance Chronic rhinosinusitis (CRS) |
| title | The nasal microbiota in health and disease: variation within and between subjects |
| title_full | The nasal microbiota in health and disease: variation within and between subjects |
| title_fullStr | The nasal microbiota in health and disease: variation within and between subjects |
| title_full_unstemmed | The nasal microbiota in health and disease: variation within and between subjects |
| title_short | The nasal microbiota in health and disease: variation within and between subjects |
| title_sort | nasal microbiota in health and disease variation within and between subjects |
| topic | 16S rRNA gene bacterial diversity Bacterial communities Bacterial abundance Chronic rhinosinusitis (CRS) |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00134/full |
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