Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications
Despite several decades of intensive research on spirurine nematodes, molecular data on some of the main lineages are still absent, which makes taxonomic classification insufficiently resolved. In the present study, we sequenced the first complete mitogenome for the family Quimperiidae, belonging to...
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MDPI AG
2021-11-01
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| author | Fanglin Chen Hong Zou Xiao Jin Dong Zhang Wenxiang Li Ming Li Shangong Wu Guitang Wang |
| author_facet | Fanglin Chen Hong Zou Xiao Jin Dong Zhang Wenxiang Li Ming Li Shangong Wu Guitang Wang |
| author_sort | Fanglin Chen |
| collection | DOAJ |
| description | Despite several decades of intensive research on spirurine nematodes, molecular data on some of the main lineages are still absent, which makes taxonomic classification insufficiently resolved. In the present study, we sequenced the first complete mitogenome for the family Quimperiidae, belonging to <i>P. sinensis</i> (Spirurina: Quimperiidae), a parasite living in the intestines of snakehead (<i>Ophiocephalus argus</i>). The circular mitogenome is 13,874 bp long, and it contains the standard nematode gene set: 22 transfer RNAs, 2 ribosomal RNAs and 12 protein-coding genes. There are also two long non-coding regions (NCR), in addition to only 8 other intergenic regions, ranging in size from 1 to 58 bp. To investigate its phylogenetic position and study the relationships among other available Spirurina, we performed the phylogenetic analysis using Bayesian inference and maximum likelihood approaches by concatenating the nucleotide sequences of all 36 genes on a dataset containing all available mitogenomes of the suborder Spirurina from NCBI and compared with gene order phylogenies using the MLGO program. Both supported the closer relationship of Ascaridoidea to Seuratoidea than to Spiruroidea. Pingus formed a sister-group with the Cucullanus genus. The results provide a new insights into the relationships within Spirurina. |
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| language | English |
| last_indexed | 2024-03-10T05:28:30Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-5edb4d1773df4c66977f0409c8a3390a2023-11-22T23:28:34ZengMDPI AGGenes2073-44252021-11-011211177210.3390/genes12111772Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic ImplicationsFanglin Chen0Hong Zou1Xiao Jin2Dong Zhang3Wenxiang Li4Ming Li5Shangong Wu6Guitang Wang7College of Science, Tibet University, Lhasa 850000, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology, Lanzhou University, Lanzhou 730000, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquaculture Disease Control, Ministry of Agriculture and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaCollege of Science, Tibet University, Lhasa 850000, ChinaDespite several decades of intensive research on spirurine nematodes, molecular data on some of the main lineages are still absent, which makes taxonomic classification insufficiently resolved. In the present study, we sequenced the first complete mitogenome for the family Quimperiidae, belonging to <i>P. sinensis</i> (Spirurina: Quimperiidae), a parasite living in the intestines of snakehead (<i>Ophiocephalus argus</i>). The circular mitogenome is 13,874 bp long, and it contains the standard nematode gene set: 22 transfer RNAs, 2 ribosomal RNAs and 12 protein-coding genes. There are also two long non-coding regions (NCR), in addition to only 8 other intergenic regions, ranging in size from 1 to 58 bp. To investigate its phylogenetic position and study the relationships among other available Spirurina, we performed the phylogenetic analysis using Bayesian inference and maximum likelihood approaches by concatenating the nucleotide sequences of all 36 genes on a dataset containing all available mitogenomes of the suborder Spirurina from NCBI and compared with gene order phylogenies using the MLGO program. Both supported the closer relationship of Ascaridoidea to Seuratoidea than to Spiruroidea. Pingus formed a sister-group with the Cucullanus genus. The results provide a new insights into the relationships within Spirurina.https://www.mdpi.com/2073-4425/12/11/1772mitogenomegene order<i>Pingus sinensis</i>Quimperiidaespirurinaphylogeny |
| spellingShingle | Fanglin Chen Hong Zou Xiao Jin Dong Zhang Wenxiang Li Ming Li Shangong Wu Guitang Wang Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications Genes mitogenome gene order <i>Pingus sinensis</i> Quimperiidae spirurina phylogeny |
| title | Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications |
| title_full | Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications |
| title_fullStr | Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications |
| title_full_unstemmed | Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications |
| title_short | Sequencing of the Complete Mitochondrial Genome of <i>Pingus sinensis</i> (Spirurina: Quimperiidae): Gene Arrangements and Phylogenetic Implications |
| title_sort | sequencing of the complete mitochondrial genome of i pingus sinensis i spirurina quimperiidae gene arrangements and phylogenetic implications |
| topic | mitogenome gene order <i>Pingus sinensis</i> Quimperiidae spirurina phylogeny |
| url | https://www.mdpi.com/2073-4425/12/11/1772 |
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