A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
<p>Abstract</p> <p>Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripot...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-04-01
|
| Series: | Cell Communication and Signaling |
| Online Access: | http://www.biosignaling.com/content/9/1/8 |
| _version_ | 1819082558290264064 |
|---|---|
| author | Edel Michael J Menchon Cristina Vaquero Jose Izpisua Belmonte Juan Carlos |
| author_facet | Edel Michael J Menchon Cristina Vaquero Jose Izpisua Belmonte Juan Carlos |
| author_sort | Edel Michael J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.</p> |
| first_indexed | 2024-12-21T20:18:35Z |
| format | Article |
| id | doaj.art-5edb9e3614e544e0851a5a893c32193a |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-12-21T20:18:35Z |
| publishDate | 2011-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-5edb9e3614e544e0851a5a893c32193a2022-12-21T18:51:33ZengBMCCell Communication and Signaling1478-811X2011-04-0191810.1186/1478-811X-9-8A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cellsEdel Michael JMenchon CristinaVaquero JoseIzpisua Belmonte Juan Carlos<p>Abstract</p> <p>Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.</p>http://www.biosignaling.com/content/9/1/8 |
| spellingShingle | Edel Michael J Menchon Cristina Vaquero Jose Izpisua Belmonte Juan Carlos A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells Cell Communication and Signaling |
| title | A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| title_full | A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| title_fullStr | A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| title_full_unstemmed | A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| title_short | A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| title_sort | protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells |
| url | http://www.biosignaling.com/content/9/1/8 |
| work_keys_str_mv | AT edelmichaelj aprotocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT menchoncristina aprotocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT vaquerojose aprotocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT izpisuabelmontejuancarlos aprotocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT edelmichaelj protocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT menchoncristina protocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT vaquerojose protocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells AT izpisuabelmontejuancarlos protocoltoassesscellcycleandapoptosisinhumanandmousepluripotentcells |