Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Abstract Background The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To co...

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Bibliographic Details
Main Authors: Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Stem Cell Research & Therapy
Subjects:
Stromal vascular fraction
Adipose tissue
Freezing protocol
Cell viability
CFU-F
Immunophenotype characterization
Online Access:http://link.springer.com/article/10.1186/s13287-018-0886-1

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