PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review
'Candidatus Liberibacter solanacearum' is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several dat...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2019-12-01
|
| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201904-0001_pcr-based-diagnostic-methods-for-candidatus-liberibacter-solanacearum-review.php |
| _version_ | 1797898483823280128 |
|---|---|
| author | Seyed Mahyar Mirmajlessi Maria Jennifer Sjölund Marika Mänd Marianne Loiseau Vincenza Ilardi Geert Haesaert Reet Karise Richard Alexander Gottsberger Jason Sumner-Kalkun Assunta Bertaccini |
| author_facet | Seyed Mahyar Mirmajlessi Maria Jennifer Sjölund Marika Mänd Marianne Loiseau Vincenza Ilardi Geert Haesaert Reet Karise Richard Alexander Gottsberger Jason Sumner-Kalkun Assunta Bertaccini |
| author_sort | Seyed Mahyar Mirmajlessi |
| collection | DOAJ |
| description | 'Candidatus Liberibacter solanacearum' is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories. |
| first_indexed | 2024-04-10T08:14:05Z |
| format | Article |
| id | doaj.art-5ee16df2b4a849229c9929468ade8926 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:14:05Z |
| publishDate | 2019-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-5ee16df2b4a849229c9929468ade89262023-02-23T03:44:27ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412019-12-0155422924210.17221/145/2018-PPSpps-201904-0001PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - ReviewSeyed Mahyar Mirmajlessi0Maria Jennifer Sjölund1Marika Mänd2Marianne Loiseau3Vincenza Ilardi4Geert Haesaert5Reet Karise6Richard Alexander Gottsberger7Jason Sumner-Kalkun8Assunta Bertaccini9Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, BelgiumScience and Advice for Scottish Agriculture (SASA), Edinburgh, UKDepartment of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, EstoniaFrench Agency for Food, Environmental and Occupational Health and Safety, Angers, FranceCentro Difesa e Certificazione, CREA, Rome, ItalyDepartment of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, BelgiumDepartment of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, EstoniaDepartment for Molecular Diagnostics of Plant Diseases, Institute for Sustainable Plant Production, Austrian Agency for Health and Food Safety (AGES), Vienna, AustriaScience and Advice for Scottish Agriculture (SASA), Edinburgh, UKDepartment of Agricultural and Food Sciences, University of Bologna, Bologna, Italy'Candidatus Liberibacter solanacearum' is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories.https://pps.agriculturejournals.cz/artkey/pps-201904-0001_pcr-based-diagnostic-methods-for-candidatus-liberibacter-solanacearum-review.phplsoleaf stuntingearly detectionidentificationin plantamolecular methods |
| spellingShingle | Seyed Mahyar Mirmajlessi Maria Jennifer Sjölund Marika Mänd Marianne Loiseau Vincenza Ilardi Geert Haesaert Reet Karise Richard Alexander Gottsberger Jason Sumner-Kalkun Assunta Bertaccini PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review Plant Protection Science lso leaf stunting early detection identification in planta molecular methods |
| title | PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review |
| title_full | PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review |
| title_fullStr | PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review |
| title_full_unstemmed | PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review |
| title_short | PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review |
| title_sort | pcr based diagnostic methods for candidatus liberibacter solanacearum review |
| topic | lso leaf stunting early detection identification in planta molecular methods |
| url | https://pps.agriculturejournals.cz/artkey/pps-201904-0001_pcr-based-diagnostic-methods-for-candidatus-liberibacter-solanacearum-review.php |
| work_keys_str_mv | AT seyedmahyarmirmajlessi pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT mariajennifersjolund pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT marikamand pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT marianneloiseau pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT vincenzailardi pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT geerthaesaert pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT reetkarise pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT richardalexandergottsberger pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT jasonsumnerkalkun pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview AT assuntabertaccini pcrbaseddiagnosticmethodsforcandidatusliberibactersolanacearumreview |