Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics
Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and prov...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15895 |
| _version_ | 1797457261545652224 |
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| author | David A. Ball Binta Jalloh Tatiana S. Karpova |
| author_facet | David A. Ball Binta Jalloh Tatiana S. Karpova |
| author_sort | David A. Ball |
| collection | DOAJ |
| description | Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes. |
| first_indexed | 2024-03-09T16:18:45Z |
| format | Article |
| id | doaj.art-5ee41f1c85ca463096c361f232b97072 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:18:45Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5ee41f1c85ca463096c361f232b970722023-11-24T15:29:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241589510.3390/ijms232415895Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule BiophysicsDavid A. Ball0Binta Jalloh1Tatiana S. Karpova2CCR/LRBGE Optical Microscopy Core, National Cancer Institute, National Institutes of Health, Bethesda, MD 20852, USACCR/LRBGE Optical Microscopy Core, National Cancer Institute, National Institutes of Health, Bethesda, MD 20852, USACCR/LRBGE Optical Microscopy Core, National Cancer Institute, National Institutes of Health, Bethesda, MD 20852, USACellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes.https://www.mdpi.com/1422-0067/23/24/15895SMTprotein dynamicstranscriptionHaloTagSNAP-Tagchromatin remodelers |
| spellingShingle | David A. Ball Binta Jalloh Tatiana S. Karpova Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics International Journal of Molecular Sciences SMT protein dynamics transcription HaloTag SNAP-Tag chromatin remodelers |
| title | Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics |
| title_full | Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics |
| title_fullStr | Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics |
| title_full_unstemmed | Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics |
| title_short | Impact of <i>Saccharomyces cerevisiae</i> on the Field of Single-Molecule Biophysics |
| title_sort | impact of i saccharomyces cerevisiae i on the field of single molecule biophysics |
| topic | SMT protein dynamics transcription HaloTag SNAP-Tag chromatin remodelers |
| url | https://www.mdpi.com/1422-0067/23/24/15895 |
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