Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand
The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2016-03-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-29-3-327.pdf |
| _version_ | 1818271903841779712 |
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| author | Somkiat Seilsuth Joo Hee Seo Hong Sik Kong Gwang Joo Jeon |
| author_facet | Somkiat Seilsuth Joo Hee Seo Hong Sik Kong Gwang Joo Jeon |
| author_sort | Somkiat Seilsuth |
| collection | DOAJ |
| description | The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average Fis value for the breeds ranged from −0.044 (ETH225) to 0.180 (SPS115), while the estimated average Fst value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy. |
| first_indexed | 2024-12-12T21:33:34Z |
| format | Article |
| id | doaj.art-5eea999f27234620b810be40158702b8 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T21:33:34Z |
| publishDate | 2016-03-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-5eea999f27234620b810be40158702b82022-12-22T00:11:14ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-03-0129332733210.5713/ajas.15.027023289Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in ThailandSomkiat Seilsuth0Joo Hee Seo1Hong Sik Kong2Gwang Joo Jeon3 Faculty of Agriculture and Life Science, Chadrakasem Rajabhat University, Bangkok 10902, Thailand Genetic Informatics Center, Hankyong National University, Anseong 456-749, .Korea Genetic Informatics Center, Hankyong National University, Anseong 456-749, .Korea Genetic Informatics Center, Hankyong National University, Anseong 456-749, .KoreaThe genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average Fis value for the breeds ranged from −0.044 (ETH225) to 0.180 (SPS115), while the estimated average Fst value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.http://www.ajas.info/upload/pdf/ajas-29-3-327.pdfMicrosatelliteGenetic DiversityDairy Goat |
| spellingShingle | Somkiat Seilsuth Joo Hee Seo Hong Sik Kong Gwang Joo Jeon Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand Asian-Australasian Journal of Animal Sciences Microsatellite Genetic Diversity Dairy Goat |
| title | Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand |
| title_full | Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand |
| title_fullStr | Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand |
| title_full_unstemmed | Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand |
| title_short | Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand |
| title_sort | microsatellite analysis of the genetic diversity and population structure in dairy goats in thailand |
| topic | Microsatellite Genetic Diversity Dairy Goat |
| url | http://www.ajas.info/upload/pdf/ajas-29-3-327.pdf |
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