Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and patho...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.986269/full |
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| author | Verônica Mollica Govoni Claudio Pigoli Eleonora Brambilla Felipe Augusto Ruiz Sueiro Rafael Torres Neto Renee Laufer-Amorim Juliany Gomes Quitzan Valeria Grieco Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves |
| author_facet | Verônica Mollica Govoni Claudio Pigoli Eleonora Brambilla Felipe Augusto Ruiz Sueiro Rafael Torres Neto Renee Laufer-Amorim Juliany Gomes Quitzan Valeria Grieco Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves |
| author_sort | Verônica Mollica Govoni |
| collection | DOAJ |
| description | The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness. |
| first_indexed | 2024-04-12T00:30:54Z |
| format | Article |
| id | doaj.art-5eeafdfb7d594396a1d497208d5f6fd0 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T00:30:54Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-5eeafdfb7d594396a1d497208d5f6fd02022-12-22T03:55:20ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-10-01910.3389/fvets.2022.986269986269Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinomaVerônica Mollica Govoni0Claudio Pigoli1Eleonora Brambilla2Felipe Augusto Ruiz Sueiro3Rafael Torres Neto4Renee Laufer-Amorim5Juliany Gomes Quitzan6Valeria Grieco7Carlos Eduardo Fonseca-Alves8Carlos Eduardo Fonseca-Alves9Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University – UNESP, Botucatu, BrazilLaboratorio di Istologia, Sede Territoriale di Milano, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Milan, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Milan, ItalyVetPat Laboratory, Campinas, BrazilVetMol Laboratory, Botucatu, BrazilDepartment of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University – UNESP, Botucatu, BrazilDepartment of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University – UNESP, Botucatu, BrazilDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Milan, ItalyDepartment of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University – UNESP, Botucatu, BrazilInstitute of Health Sciences, Paulista University – UNIP, Bauru, BrazilThe bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness.https://www.frontiersin.org/articles/10.3389/fvets.2022.986269/fullcancerdogbiomarkerimmunohistochemistryneoplasia |
| spellingShingle | Verônica Mollica Govoni Claudio Pigoli Eleonora Brambilla Felipe Augusto Ruiz Sueiro Rafael Torres Neto Renee Laufer-Amorim Juliany Gomes Quitzan Valeria Grieco Carlos Eduardo Fonseca-Alves Carlos Eduardo Fonseca-Alves Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma Frontiers in Veterinary Science cancer dog biomarker immunohistochemistry neoplasia |
| title | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| title_full | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| title_fullStr | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| title_full_unstemmed | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| title_short | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| title_sort | caveolin 1 gata 3 and ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
| topic | cancer dog biomarker immunohistochemistry neoplasia |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.986269/full |
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