Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpres...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709840/?tool=EBI |
| _version_ | 1819240612414619648 |
|---|---|
| author | Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin |
| author_facet | Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin |
| author_sort | Yong Zhou |
| collection | DOAJ |
| description | As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice. |
| first_indexed | 2024-12-23T14:10:47Z |
| format | Article |
| id | doaj.art-5eec3f7256a3487b9f60a1b21c0b952f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:10:47Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5eec3f7256a3487b9f60a1b21c0b952f2022-12-21T17:44:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9421010.1371/journal.pone.0094210Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.Yong ZhouLi LiuWeifeng HuangMeng YuanFei ZhouXianghua LiYongjun LinAs a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709840/?tool=EBI |
| spellingShingle | Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. PLoS ONE |
| title | Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. |
| title_full | Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. |
| title_fullStr | Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. |
| title_full_unstemmed | Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. |
| title_short | Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. |
| title_sort | overexpression of ossweet5 in rice causes growth retardation and precocious senescence |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709840/?tool=EBI |
| work_keys_str_mv | AT yongzhou overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT liliu overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT weifenghuang overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT mengyuan overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT feizhou overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT xianghuali overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT yongjunlin overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence |