Histology of the Ovary of the Laying Hen (Gallus domesticus)
The laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/4/4/66 |
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| author | K. Denise Apperson Karyn E. Bird Gita Cherian Christiane V. Löhr |
| author_facet | K. Denise Apperson Karyn E. Bird Gita Cherian Christiane V. Löhr |
| author_sort | K. Denise Apperson |
| collection | DOAJ |
| description | The laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and proximal oviduct to establish baselines from which lesions associated with ovarian cancer can be more readily identified. Ovaries and oviducts were collected from 18-month-old laying hens (n = 18) and fixed in 10% neutral buffered formalin. Hematoxylin- and eosin-stained sections were examined by light microscopy. Both post-ovulatory follicular regression and atresia of small follicles produce remnant clusters of vacuolated cells with no histological evidence that scar tissue persists. Infiltrates of heterophils are associated with atresia of small follicles, a relationship not previously documented in laying hen ovaries. Because these tissues can be mistaken for cancerous lesions, we present a detailed histological description of remnant Wolffian tissues in the laying hen ovary. Immunohistochemical staining for pancytokeratin produced a positive response in ovarian surface epithelium and staining for vimentin produced a positive response in granulosa cells of follicles. Epithelial cells lining glands of the remnant epoöphoron had a positive response to both pancytokeratin and vimentin, a result also observed in women. |
| first_indexed | 2024-12-14T09:33:23Z |
| format | Article |
| id | doaj.art-5eecfdbb290f4a4caebf0a1d7f719835 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-12-14T09:33:23Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-5eecfdbb290f4a4caebf0a1d7f7198352022-12-21T23:08:01ZengMDPI AGVeterinary Sciences2306-73812017-12-01446610.3390/vetsci4040066vetsci4040066Histology of the Ovary of the Laying Hen (Gallus domesticus)K. Denise Apperson0Karyn E. Bird1Gita Cherian2Christiane V. Löhr3College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USACollege of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USADepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USACollege of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USAThe laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and proximal oviduct to establish baselines from which lesions associated with ovarian cancer can be more readily identified. Ovaries and oviducts were collected from 18-month-old laying hens (n = 18) and fixed in 10% neutral buffered formalin. Hematoxylin- and eosin-stained sections were examined by light microscopy. Both post-ovulatory follicular regression and atresia of small follicles produce remnant clusters of vacuolated cells with no histological evidence that scar tissue persists. Infiltrates of heterophils are associated with atresia of small follicles, a relationship not previously documented in laying hen ovaries. Because these tissues can be mistaken for cancerous lesions, we present a detailed histological description of remnant Wolffian tissues in the laying hen ovary. Immunohistochemical staining for pancytokeratin produced a positive response in ovarian surface epithelium and staining for vimentin produced a positive response in granulosa cells of follicles. Epithelial cells lining glands of the remnant epoöphoron had a positive response to both pancytokeratin and vimentin, a result also observed in women.https://www.mdpi.com/2306-7381/4/4/66laying henepithelial ovarian cancerhistologyrete ovariiepoöphoronovarian surface epithelium |
| spellingShingle | K. Denise Apperson Karyn E. Bird Gita Cherian Christiane V. Löhr Histology of the Ovary of the Laying Hen (Gallus domesticus) Veterinary Sciences laying hen epithelial ovarian cancer histology rete ovarii epoöphoron ovarian surface epithelium |
| title | Histology of the Ovary of the Laying Hen (Gallus domesticus) |
| title_full | Histology of the Ovary of the Laying Hen (Gallus domesticus) |
| title_fullStr | Histology of the Ovary of the Laying Hen (Gallus domesticus) |
| title_full_unstemmed | Histology of the Ovary of the Laying Hen (Gallus domesticus) |
| title_short | Histology of the Ovary of the Laying Hen (Gallus domesticus) |
| title_sort | histology of the ovary of the laying hen gallus domesticus |
| topic | laying hen epithelial ovarian cancer histology rete ovarii epoöphoron ovarian surface epithelium |
| url | https://www.mdpi.com/2306-7381/4/4/66 |
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