The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium
This study examined whether a polyphenol-rich extract from the berries of <i>Aronia melanocarpa</i> L. (AE; chokeberries) may protect from the impact of cadmium (Cd) on the metabolism of collagen in the liver. The study was conducted in an experimental model (rats that were fed a diet co...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/12/9/2766 |
| _version_ | 1797553991191625728 |
|---|---|
| author | Magdalena Kozłowska Małgorzata M. Brzóska Joanna Rogalska Anna Galicka |
| author_facet | Magdalena Kozłowska Małgorzata M. Brzóska Joanna Rogalska Anna Galicka |
| author_sort | Magdalena Kozłowska |
| collection | DOAJ |
| description | This study examined whether a polyphenol-rich extract from the berries of <i>Aronia melanocarpa</i> L. (AE; chokeberries) may protect from the impact of cadmium (Cd) on the metabolism of collagen in the liver. The study was conducted in an experimental model (rats that were fed a diet containing 1 or 5 mg Cd/kg for 3–24 months) of human exposure to this xenobiotic during a lifetime. The concentration of total collagen and the expression of collagen types I and III at the mRNA and protein levels, as well as the concentrations of matrix metalloproteinases (MMP-1 and MMP-2) and their tissue inhibitors (TIMP-1 and TIMP-2), were assayed. The administration of Cd and/or AE had only a slight and temporary impact on the concentration of total collagen in the liver. The supplementation with AE significantly prevented Cd-mediated changes in the expression of collagen types I and III at the mRNA and protein levels and their ratio (collagen III/collagen I), as well as a rise in the concentrations of MMPs and TIMPs in this organ. The results allow the conclusion that the intake of chokeberry products in the case of Cd intoxication may be effective in prevention from this xenobiotic-induced disturbance in collagen homeostasis in the liver. |
| first_indexed | 2024-03-10T16:25:26Z |
| format | Article |
| id | doaj.art-5eee52415756444fa3dd3dca6d504572 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T16:25:26Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-5eee52415756444fa3dd3dca6d5045722023-11-20T13:18:21ZengMDPI AGNutrients2072-66432020-09-01129276610.3390/nu12092766The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to CadmiumMagdalena Kozłowska0Małgorzata M. Brzóska1Joanna Rogalska2Anna Galicka3Department of Toxicology, Medical University of Bialystok, Adama Mickiewicza 2C street, 15-222 Bialystok, PolandDepartment of Toxicology, Medical University of Bialystok, Adama Mickiewicza 2C street, 15-222 Bialystok, PolandDepartment of Toxicology, Medical University of Bialystok, Adama Mickiewicza 2C street, 15-222 Bialystok, PolandDepartment of Medical Chemistry, Medical University of Bialystok, Adama Mickiewicza 2A street, 15-222 Bialystok, PolandThis study examined whether a polyphenol-rich extract from the berries of <i>Aronia melanocarpa</i> L. (AE; chokeberries) may protect from the impact of cadmium (Cd) on the metabolism of collagen in the liver. The study was conducted in an experimental model (rats that were fed a diet containing 1 or 5 mg Cd/kg for 3–24 months) of human exposure to this xenobiotic during a lifetime. The concentration of total collagen and the expression of collagen types I and III at the mRNA and protein levels, as well as the concentrations of matrix metalloproteinases (MMP-1 and MMP-2) and their tissue inhibitors (TIMP-1 and TIMP-2), were assayed. The administration of Cd and/or AE had only a slight and temporary impact on the concentration of total collagen in the liver. The supplementation with AE significantly prevented Cd-mediated changes in the expression of collagen types I and III at the mRNA and protein levels and their ratio (collagen III/collagen I), as well as a rise in the concentrations of MMPs and TIMPs in this organ. The results allow the conclusion that the intake of chokeberry products in the case of Cd intoxication may be effective in prevention from this xenobiotic-induced disturbance in collagen homeostasis in the liver.https://www.mdpi.com/2072-6643/12/9/2766<i>Aronia melanocarpa</i> L.cadmiumchokeberrycollagenlivermatrix metalloproteinases |
| spellingShingle | Magdalena Kozłowska Małgorzata M. Brzóska Joanna Rogalska Anna Galicka The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium Nutrients <i>Aronia melanocarpa</i> L. cadmium chokeberry collagen liver matrix metalloproteinases |
| title | The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium |
| title_full | The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium |
| title_fullStr | The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium |
| title_full_unstemmed | The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium |
| title_short | The Impact of a Polyphenol-Rich Extract from the Berries of <i>Aronia melanocarpa</i> L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium |
| title_sort | impact of a polyphenol rich extract from the berries of i aronia melanocarpa i l on collagen metabolism in the liver a study in an in vivo model of human environmental exposure to cadmium |
| topic | <i>Aronia melanocarpa</i> L. cadmium chokeberry collagen liver matrix metalloproteinases |
| url | https://www.mdpi.com/2072-6643/12/9/2766 |
| work_keys_str_mv | AT magdalenakozłowska theimpactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT małgorzatambrzoska theimpactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT joannarogalska theimpactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT annagalicka theimpactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT magdalenakozłowska impactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT małgorzatambrzoska impactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT joannarogalska impactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium AT annagalicka impactofapolyphenolrichextractfromtheberriesofiaroniamelanocarpailoncollagenmetabolismintheliverastudyinaninvivomodelofhumanenvironmentalexposuretocadmium |