Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production
A pre-culture stage is required to obtain robustly-dividing cells with high hydrogen (H<sub>2</sub>) production capabilities. However, a step-by-step process for biohydrogen production is scarcely reported, mainly from palm oil wastewater. Therefore, this study developed a guideline to f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/2/127 |
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| author | Bidattul Syirat Zainal Sabrina Zaini Ali Akbar Zinatizadeh Nuruol Syuhadaa Mohd Shaliza Ibrahim Pin Jern Ker Hassan Mohamed |
| author_facet | Bidattul Syirat Zainal Sabrina Zaini Ali Akbar Zinatizadeh Nuruol Syuhadaa Mohd Shaliza Ibrahim Pin Jern Ker Hassan Mohamed |
| author_sort | Bidattul Syirat Zainal |
| collection | DOAJ |
| description | A pre-culture stage is required to obtain robustly-dividing cells with high hydrogen (H<sub>2</sub>) production capabilities. However, a step-by-step process for biohydrogen production is scarcely reported, mainly from palm oil wastewater. Therefore, this study developed a guideline to find the best inoculum heat treatment conditions and implement the selected conditions for biohydrogen production using palm oil wastewater. This study used raw palm oil mill effluent (POME) and POME sludge as substrate and inoculum, respectively. Our findings reveal that 80 °C and 30 min were the best conditions for inoculum heat treatment. When testing the conditions on POME sludge and inoculating with raw POME (28 g COD/L) at 37 °C (reaction temperature), 24 h (reaction time), and pH 5.5, 34 mL H<sub>2</sub>/d was recorded. A slight increase (1.1-fold) was observed compared to 5 g COD/L POME co-digested with 5 g/L glucose (31 mL H<sub>2</sub>/d). This discovery indicates that raw POME is a potential source for biohydrogen production under anaerobic fermentation and can be directly used as substrate up to 30 g COD/L. The proposed guideline could also be implemented for different organic wastes for biohydrogen production study. |
| first_indexed | 2024-03-11T08:50:49Z |
| format | Article |
| id | doaj.art-5eee752485b6424099ec47ae0cdee8b6 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T08:50:49Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-5eee752485b6424099ec47ae0cdee8b62023-11-16T20:25:50ZengMDPI AGFermentation2311-56372023-01-019212710.3390/fermentation9020127Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen ProductionBidattul Syirat Zainal0Sabrina Zaini1Ali Akbar Zinatizadeh2Nuruol Syuhadaa Mohd3Shaliza Ibrahim4Pin Jern Ker5Hassan Mohamed6Institute of Sustainable Energy, Universiti Tenaga Nasional, Jalan IKRAM-UNITEN, Kajang 43000, Selangor, MalaysiaDepartment of Civil Engineering, Faculty of Engineering, University of Malaya, Jln Profesor Diraja Ungku Aziz, Seksyen 13, Kuala Lumpur 50603, Selangor, MalaysiaDepartment of Applied Chemistry, Faculty of Chemistry, Razi University, Kermanshah 6714414971, IranDepartment of Civil Engineering, Faculty of Engineering, University of Malaya, Jln Profesor Diraja Ungku Aziz, Seksyen 13, Kuala Lumpur 50603, Selangor, MalaysiaInstitute of Ocean & Earth Sciences, University of Malaya, Jln Profesor Diraja Ungku Aziz, Seksyen 13, Kuala Lumpur 50603, Selangor, MalaysiaInstitute of Sustainable Energy, Universiti Tenaga Nasional, Jalan IKRAM-UNITEN, Kajang 43000, Selangor, MalaysiaInstitute of Sustainable Energy, Universiti Tenaga Nasional, Jalan IKRAM-UNITEN, Kajang 43000, Selangor, MalaysiaA pre-culture stage is required to obtain robustly-dividing cells with high hydrogen (H<sub>2</sub>) production capabilities. However, a step-by-step process for biohydrogen production is scarcely reported, mainly from palm oil wastewater. Therefore, this study developed a guideline to find the best inoculum heat treatment conditions and implement the selected conditions for biohydrogen production using palm oil wastewater. This study used raw palm oil mill effluent (POME) and POME sludge as substrate and inoculum, respectively. Our findings reveal that 80 °C and 30 min were the best conditions for inoculum heat treatment. When testing the conditions on POME sludge and inoculating with raw POME (28 g COD/L) at 37 °C (reaction temperature), 24 h (reaction time), and pH 5.5, 34 mL H<sub>2</sub>/d was recorded. A slight increase (1.1-fold) was observed compared to 5 g COD/L POME co-digested with 5 g/L glucose (31 mL H<sub>2</sub>/d). This discovery indicates that raw POME is a potential source for biohydrogen production under anaerobic fermentation and can be directly used as substrate up to 30 g COD/L. The proposed guideline could also be implemented for different organic wastes for biohydrogen production study.https://www.mdpi.com/2311-5637/9/2/127anaerobic digestionbiohydrogenheat treatmentpalm oilrenewable sourcesenergy |
| spellingShingle | Bidattul Syirat Zainal Sabrina Zaini Ali Akbar Zinatizadeh Nuruol Syuhadaa Mohd Shaliza Ibrahim Pin Jern Ker Hassan Mohamed Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production Fermentation anaerobic digestion biohydrogen heat treatment palm oil renewable sources energy |
| title | Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production |
| title_full | Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production |
| title_fullStr | Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production |
| title_full_unstemmed | Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production |
| title_short | Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production |
| title_sort | preliminary investigation of different types of inoculums and substrate preparation for biohydrogen production |
| topic | anaerobic digestion biohydrogen heat treatment palm oil renewable sources energy |
| url | https://www.mdpi.com/2311-5637/9/2/127 |
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