Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice
Abstract Disturbances of attention are a common behavioral feature associated with neuropsychiatric disorders with largely unknown underlying causes. We previously developed an object-based attention test (OBAT) as a simple and practical method for evaluating attention in mice. Since its establishme...
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Molecular Brain |
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| Online Access: | https://doi.org/10.1186/s13041-020-00711-4 |
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| author | Bolati Wulaer Kazuo Kunisawa Hisayoshi Kubota Willy Jaya Suento Kuniaki Saito Akihiro Mouri Toshitaka Nabeshima |
| author_facet | Bolati Wulaer Kazuo Kunisawa Hisayoshi Kubota Willy Jaya Suento Kuniaki Saito Akihiro Mouri Toshitaka Nabeshima |
| author_sort | Bolati Wulaer |
| collection | DOAJ |
| description | Abstract Disturbances of attention are a common behavioral feature associated with neuropsychiatric disorders with largely unknown underlying causes. We previously developed an object-based attention test (OBAT) as a simple and practical method for evaluating attention in mice. Since its establishment, the test has become a popular method for assessing attention and related underlying mechanisms in various mouse models. However, the underlying neuronal network involved in this test has yet to be studied. The purpose of this study was to identify the principal brain regions activated in the OBAT. Accordingly, C57BL/6J mice were subjected to the OBAT and thereafter prepared for immunohistochemical quantification of c-Fos, an immediate early gene that is frequently used as a marker of neuronal activity, in 13 different brain regions. The number of c-Fos-positive cells was significantly higher in the prefrontal cortex (PFC), dorsomedial striatum (DMS), and dentate gyrus (DG) in the test group as compared to the control group. The neuronal activation of these brain regions during the OBAT indicates that these brain regions are necessary for the regulation of attention in this test. This was supported by excitotoxic lesioning of these brain regions, leading to impaired attention without causing locomotor dysfunction. This study is one of the first attempts to analyze the brain regions that regulate attention in the OBAT. These findings provide an initial insight into the role of these brain regions and ideas for studying the underlying neural and molecular mechanisms. |
| first_indexed | 2024-12-17T01:02:21Z |
| format | Article |
| id | doaj.art-5eeeeb7ee8914bdd9b9f5090864fe0d4 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-17T01:02:21Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-5eeeeb7ee8914bdd9b9f5090864fe0d42022-12-21T22:09:24ZengBMCMolecular Brain1756-66062020-12-011311410.1186/s13041-020-00711-4Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in miceBolati Wulaer0Kazuo Kunisawa1Hisayoshi Kubota2Willy Jaya Suento3Kuniaki Saito4Akihiro Mouri5Toshitaka Nabeshima6Advanced Diagnostic System Research Laboratory, Fujita Health University Graduate School of Health ScienceDepartment of Regulatory Science for Evaluation & Development of Pharmaceuticals & Devices, Fujita Health University Graduate School of Health ScienceDepartment of Regulatory Science for Evaluation & Development of Pharmaceuticals & Devices, Fujita Health University Graduate School of Health ScienceDepartment of Disease Control and Prevention, Fujita Health University Graduate School of Health ScienceDepartment of Disease Control and Prevention, Fujita Health University Graduate School of Health ScienceDepartment of Regulatory Science for Evaluation & Development of Pharmaceuticals & Devices, Fujita Health University Graduate School of Health ScienceAdvanced Diagnostic System Research Laboratory, Fujita Health University Graduate School of Health ScienceAbstract Disturbances of attention are a common behavioral feature associated with neuropsychiatric disorders with largely unknown underlying causes. We previously developed an object-based attention test (OBAT) as a simple and practical method for evaluating attention in mice. Since its establishment, the test has become a popular method for assessing attention and related underlying mechanisms in various mouse models. However, the underlying neuronal network involved in this test has yet to be studied. The purpose of this study was to identify the principal brain regions activated in the OBAT. Accordingly, C57BL/6J mice were subjected to the OBAT and thereafter prepared for immunohistochemical quantification of c-Fos, an immediate early gene that is frequently used as a marker of neuronal activity, in 13 different brain regions. The number of c-Fos-positive cells was significantly higher in the prefrontal cortex (PFC), dorsomedial striatum (DMS), and dentate gyrus (DG) in the test group as compared to the control group. The neuronal activation of these brain regions during the OBAT indicates that these brain regions are necessary for the regulation of attention in this test. This was supported by excitotoxic lesioning of these brain regions, leading to impaired attention without causing locomotor dysfunction. This study is one of the first attempts to analyze the brain regions that regulate attention in the OBAT. These findings provide an initial insight into the role of these brain regions and ideas for studying the underlying neural and molecular mechanisms.https://doi.org/10.1186/s13041-020-00711-4Prefrontal cortexStriatumDentate gyrusAttentionOBATc-Fos |
| spellingShingle | Bolati Wulaer Kazuo Kunisawa Hisayoshi Kubota Willy Jaya Suento Kuniaki Saito Akihiro Mouri Toshitaka Nabeshima Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice Molecular Brain Prefrontal cortex Striatum Dentate gyrus Attention OBAT c-Fos |
| title | Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice |
| title_full | Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice |
| title_fullStr | Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice |
| title_full_unstemmed | Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice |
| title_short | Prefrontal cortex, dorsomedial striatum, and dentate gyrus are necessary in the object-based attention test in mice |
| title_sort | prefrontal cortex dorsomedial striatum and dentate gyrus are necessary in the object based attention test in mice |
| topic | Prefrontal cortex Striatum Dentate gyrus Attention OBAT c-Fos |
| url | https://doi.org/10.1186/s13041-020-00711-4 |
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