miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population
Alzheimer’s disease (AD) is the most frequent cause of dementia worldwide and represents one of the leading factors for severe disability in older persons. Although its etiology is not fully known yet, AD may develop due to multiple factors, including inflammation and oxidative stress, conditions wh...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/3/634 |
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| author | Virginia Boccardi Giulia Poli Roberta Cecchetti Patrizia Bastiani Michela Scamosci Marta Febo Emanuela Mazzon Stefano Bruscoli Stefano Brancorsini Patrizia Mecocci |
| author_facet | Virginia Boccardi Giulia Poli Roberta Cecchetti Patrizia Bastiani Michela Scamosci Marta Febo Emanuela Mazzon Stefano Bruscoli Stefano Brancorsini Patrizia Mecocci |
| author_sort | Virginia Boccardi |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is the most frequent cause of dementia worldwide and represents one of the leading factors for severe disability in older persons. Although its etiology is not fully known yet, AD may develop due to multiple factors, including inflammation and oxidative stress, conditions where microRNAs (miRNAs) seem to play a pivotal role as a molecular switch. All these aspects may be modulated by nutritional factors. Among them, vitamin E has been widely studied in AD, given the plausibility of its various biological functions in influencing neurodegeneration. From a cohort of old-aged people, we measured eight vitamin E forms (tocopherols and tocotrienols), thirty cytokines/chemokines, and thirteen exosome-extracted miRNAs in plasma of subjects suffering from subjects affected by AD and age-matched healthy controls (HC). The sample population included 80 subjects (40 AD and 40 HC) with a mean age of 77.6 ± 3.8 years, mostly women (45; 56.2%). Of the vitamin E forms, only α-tocopherol differed between groups, with significantly lower levels in AD. Regarding the examined inflammatory molecules, G-CSF, GM-CSF, INF-α2, IL-3, and IL-8 were significantly higher and IL-17 lower in AD than HC. Among all miRNAs examined, AD showed downregulation of miR-9, miR-21, miR29-b, miR-122, and miR-132 compared to controls. MiR-122 positively and significantly correlated with some inflammatory molecules (GM-CSF, INF-α2, IL-1α, IL-8, and MIP-1β) as well as with α-tocopherol even after correction for age and gender. A final binary logistic regression analysis showed that α-tocopherol serum levels were associated with a higher AD probability and partially mediated by miR-122. Our results suggest an interplay between α-tocopherol, inflammatory molecules, and microRNAs in AD, where miR-122 may be a good candidate as modulating factor. |
| first_indexed | 2024-03-11T09:30:23Z |
| format | Article |
| id | doaj.art-5eefea927b054ddc9d482d5ead4ba2f6 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T09:30:23Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-5eefea927b054ddc9d482d5ead4ba2f62023-11-16T17:39:43ZengMDPI AGNutrients2072-66432023-01-0115363410.3390/nu15030634miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age PopulationVirginia Boccardi0Giulia Poli1Roberta Cecchetti2Patrizia Bastiani3Michela Scamosci4Marta Febo5Emanuela Mazzon6Stefano Bruscoli7Stefano Brancorsini8Patrizia Mecocci9Institute of Gerontology and Geriatrics, Department of Medicine and Surgery, University of Perugia, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, 05100 Terni, ItalyInstitute of Gerontology and Geriatrics, Department of Medicine and Surgery, University of Perugia, 06132 Perugia, ItalyInstitute of Gerontology and Geriatrics, Department of Medicine and Surgery, University of Perugia, 06132 Perugia, ItalyInstitute of Gerontology and Geriatrics, Department of Medicine and Surgery, University of Perugia, 06132 Perugia, ItalyDepartment of Medicine and Surgery, Section of Pharmacology, University of Perugia, 05100 Terni, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Medicine and Surgery, Section of Pharmacology, University of Perugia, 05100 Terni, ItalyDepartment of Medicine and Surgery, University of Perugia, 05100 Terni, ItalyInstitute of Gerontology and Geriatrics, Department of Medicine and Surgery, University of Perugia, 06132 Perugia, ItalyAlzheimer’s disease (AD) is the most frequent cause of dementia worldwide and represents one of the leading factors for severe disability in older persons. Although its etiology is not fully known yet, AD may develop due to multiple factors, including inflammation and oxidative stress, conditions where microRNAs (miRNAs) seem to play a pivotal role as a molecular switch. All these aspects may be modulated by nutritional factors. Among them, vitamin E has been widely studied in AD, given the plausibility of its various biological functions in influencing neurodegeneration. From a cohort of old-aged people, we measured eight vitamin E forms (tocopherols and tocotrienols), thirty cytokines/chemokines, and thirteen exosome-extracted miRNAs in plasma of subjects suffering from subjects affected by AD and age-matched healthy controls (HC). The sample population included 80 subjects (40 AD and 40 HC) with a mean age of 77.6 ± 3.8 years, mostly women (45; 56.2%). Of the vitamin E forms, only α-tocopherol differed between groups, with significantly lower levels in AD. Regarding the examined inflammatory molecules, G-CSF, GM-CSF, INF-α2, IL-3, and IL-8 were significantly higher and IL-17 lower in AD than HC. Among all miRNAs examined, AD showed downregulation of miR-9, miR-21, miR29-b, miR-122, and miR-132 compared to controls. MiR-122 positively and significantly correlated with some inflammatory molecules (GM-CSF, INF-α2, IL-1α, IL-8, and MIP-1β) as well as with α-tocopherol even after correction for age and gender. A final binary logistic regression analysis showed that α-tocopherol serum levels were associated with a higher AD probability and partially mediated by miR-122. Our results suggest an interplay between α-tocopherol, inflammatory molecules, and microRNAs in AD, where miR-122 may be a good candidate as modulating factor.https://www.mdpi.com/2072-6643/15/3/634aginginflammationoxidative stressmiRNAsvitamin E |
| spellingShingle | Virginia Boccardi Giulia Poli Roberta Cecchetti Patrizia Bastiani Michela Scamosci Marta Febo Emanuela Mazzon Stefano Bruscoli Stefano Brancorsini Patrizia Mecocci miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population Nutrients aging inflammation oxidative stress miRNAs vitamin E |
| title | miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population |
| title_full | miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population |
| title_fullStr | miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population |
| title_full_unstemmed | miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population |
| title_short | miRNAs and Alzheimer’s Disease: Exploring the Role of Inflammation and Vitamin E in an Old-Age Population |
| title_sort | mirnas and alzheimer s disease exploring the role of inflammation and vitamin e in an old age population |
| topic | aging inflammation oxidative stress miRNAs vitamin E |
| url | https://www.mdpi.com/2072-6643/15/3/634 |
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