Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies
Objective: To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia. Methods: Between January 1997 and March 2004 a total of 15100 patients were referred to the Centro de Hematologia de São Paulo for hematological investigation of several diagnoses (an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2011-06-01
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| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000200130&tlng=en |
| _version_ | 1818891710702288896 |
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| author | João Carlos de Campos Guerra Ruth Hissae Kanayama Sonia Tsukasa Nozawa Márcia Regina Ioshida Irina Yoko Takiri Robson José Lazaro Nelson Hamerschlak Luiz Gastão Mange Rosenfeld Celso Carlos de Campos Guerra Nydia Strachman Bacal |
| author_facet | João Carlos de Campos Guerra Ruth Hissae Kanayama Sonia Tsukasa Nozawa Márcia Regina Ioshida Irina Yoko Takiri Robson José Lazaro Nelson Hamerschlak Luiz Gastão Mange Rosenfeld Celso Carlos de Campos Guerra Nydia Strachman Bacal |
| author_sort | João Carlos de Campos Guerra |
| collection | DOAJ |
| description | Objective: To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia. Methods: Between January 1997 and March 2004 a total of 15100 patients were referred to the Centro de Hematologia de São Paulo for hematological investigation of several diagnoses (anemia, leukopenia, thrombocytopenia, coagulation abnormalities, adenomegaly, leukemia and others). Of those, 1057 were referred because of thrombocytopenia and were divided into two groups: Group Idiopathic thrombocytopenic purpura, with no identifiable cause; and Group Other thrombocytopenia, which included low normal platelet counts cause to be established, hepatitis C and HIV infection, hypersplenism, EDTA-induced artifacts, laboratory error, and other causes. Flow cytometry immunophenotyping was done in 115 cases to identify platelet autoantibodies (direct method). Results: Of the total number of patients, 1057 (7%) presented low platelet counts, 670 were females (63.4%) and age range of one to 75 years. Of the 115 cases (9.7%) submitted to immunophenotyping, the results were positive in 40% and the test was inconclusive in 5%. Idiopathic thrombocytopenic purpura was found in 52% of patients, more often in women. Hepatitis C virus infection was found in 7% and HIV infection in 1%. Low normal platelet counts were found in 17%, laboratory errors in 6%, and laboratory artifacts in 1% of cases. Platelet autoantibodies were found in 76.9% of all idiopathic thrombocytopenic purpura cases. It was negative in 83.3% of the low normal counts. Conclusion: antiplatelet autoantibodies when present help to diagnose idiopathic thrombocytopenic purpura. When absent, suggest other causes of thrombocytopenia. |
| first_indexed | 2024-12-19T17:45:08Z |
| format | Article |
| id | doaj.art-5ef13c06b15b4ab1b329eba326195bb5 |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-19T17:45:08Z |
| publishDate | 2011-06-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-5ef13c06b15b4ab1b329eba326195bb52022-12-21T20:12:05ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852011-06-019213013410.1590/s1679-45082011ao1846Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodiesJoão Carlos de Campos GuerraRuth Hissae KanayamaSonia Tsukasa NozawaMárcia Regina IoshidaIrina Yoko TakiriRobson José LazaroNelson HamerschlakLuiz Gastão Mange RosenfeldCelso Carlos de Campos GuerraNydia Strachman BacalObjective: To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia. Methods: Between January 1997 and March 2004 a total of 15100 patients were referred to the Centro de Hematologia de São Paulo for hematological investigation of several diagnoses (anemia, leukopenia, thrombocytopenia, coagulation abnormalities, adenomegaly, leukemia and others). Of those, 1057 were referred because of thrombocytopenia and were divided into two groups: Group Idiopathic thrombocytopenic purpura, with no identifiable cause; and Group Other thrombocytopenia, which included low normal platelet counts cause to be established, hepatitis C and HIV infection, hypersplenism, EDTA-induced artifacts, laboratory error, and other causes. Flow cytometry immunophenotyping was done in 115 cases to identify platelet autoantibodies (direct method). Results: Of the total number of patients, 1057 (7%) presented low platelet counts, 670 were females (63.4%) and age range of one to 75 years. Of the 115 cases (9.7%) submitted to immunophenotyping, the results were positive in 40% and the test was inconclusive in 5%. Idiopathic thrombocytopenic purpura was found in 52% of patients, more often in women. Hepatitis C virus infection was found in 7% and HIV infection in 1%. Low normal platelet counts were found in 17%, laboratory errors in 6%, and laboratory artifacts in 1% of cases. Platelet autoantibodies were found in 76.9% of all idiopathic thrombocytopenic purpura cases. It was negative in 83.3% of the low normal counts. Conclusion: antiplatelet autoantibodies when present help to diagnose idiopathic thrombocytopenic purpura. When absent, suggest other causes of thrombocytopenia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000200130&tlng=enFlow cytometryBlood platelets |
| spellingShingle | João Carlos de Campos Guerra Ruth Hissae Kanayama Sonia Tsukasa Nozawa Márcia Regina Ioshida Irina Yoko Takiri Robson José Lazaro Nelson Hamerschlak Luiz Gastão Mange Rosenfeld Celso Carlos de Campos Guerra Nydia Strachman Bacal Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies Einstein (São Paulo) Flow cytometry Blood platelets |
| title | Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies |
| title_full | Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies |
| title_fullStr | Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies |
| title_full_unstemmed | Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies |
| title_short | Thrombocytopenia: diagnosis with flow cytometry and antiplatelet antibodies |
| title_sort | thrombocytopenia diagnosis with flow cytometry and antiplatelet antibodies |
| topic | Flow cytometry Blood platelets |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000200130&tlng=en |
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