Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance
In the coming years, climate change is likely to increase the frequency and intensity of heatwaves. In many organisms, heat stress provokes physiological perturbations and can lead to decreased male fertility. Bumblebees are endo-heterothermic but display interspecific differences in thermotolerance...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2023-11-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.231389 |
| _version_ | 1797361003484151808 |
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| author | Baptiste Martinet Kimberly Przybyla Corentin Decroo Ruddy Wattiez Serge Aron |
| author_facet | Baptiste Martinet Kimberly Przybyla Corentin Decroo Ruddy Wattiez Serge Aron |
| author_sort | Baptiste Martinet |
| collection | DOAJ |
| description | In the coming years, climate change is likely to increase the frequency and intensity of heatwaves. In many organisms, heat stress provokes physiological perturbations and can lead to decreased male fertility. Bumblebees are endo-heterothermic but display interspecific differences in thermotolerance that could have conservation implications. For the species of concern Bombus magnus, exposure to high temperatures can severely reduce sperm quality and, consequently, reproductive success. Such is not the case for B. terrestris, a ubiquitous species. To decipher the mechanisms at play, we characterized the seminal fluid proteomes of the two species. We quantified 1121 proteins, of which 522 were differentially expressed between B. terrestris and B. magnus. Several proteins with protective functions, such as proteases, antioxidant proteins and various heat-shock proteins, were present at higher levels in B. terrestris than in B. magnus under both control and heat-stress conditions. The same was true for proteins involved in cellular homeostasis, immunity, lipid/sugar metabolism and thermotolerance. Furthermore, proteins involved in the capture and elimination of reactive oxygen species also occurred at much high levels in B. terrestris. Overall, these results clearly indicate differences in the seminal proteome of the more thermotolerant B. terrestris versus B. magnus. The differences may contribute to explaining interspecific differences in sperm survival. |
| first_indexed | 2024-03-08T15:47:43Z |
| format | Article |
| id | doaj.art-5ef2bebf71244b1aa715dbd5c70dc353 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-03-08T15:47:43Z |
| publishDate | 2023-11-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-5ef2bebf71244b1aa715dbd5c70dc3532024-01-09T09:26:53ZengThe Royal SocietyRoyal Society Open Science2054-57032023-11-01101110.1098/rsos.231389Proteomic differences in seminal fluid of social insects whose sperm differ in heat toleranceBaptiste Martinet0Kimberly Przybyla1Corentin Decroo2Ruddy Wattiez3Serge Aron4Evolutionary Biology & Ecology, Université Libre de Bruxelles, Avenue Paul Héger - CP 160/12, 1000 Bruxelles, BelgiumDepartment of Zoology, Université de Mons, 7000 Mons, BelgiumDepartment of Proteomics and Microbiology, Université de Mons, 7000 Mons, BelgiumDepartment of Proteomics and Microbiology, Université de Mons, 7000 Mons, BelgiumEvolutionary Biology & Ecology, Université Libre de Bruxelles, Avenue Paul Héger - CP 160/12, 1000 Bruxelles, BelgiumIn the coming years, climate change is likely to increase the frequency and intensity of heatwaves. In many organisms, heat stress provokes physiological perturbations and can lead to decreased male fertility. Bumblebees are endo-heterothermic but display interspecific differences in thermotolerance that could have conservation implications. For the species of concern Bombus magnus, exposure to high temperatures can severely reduce sperm quality and, consequently, reproductive success. Such is not the case for B. terrestris, a ubiquitous species. To decipher the mechanisms at play, we characterized the seminal fluid proteomes of the two species. We quantified 1121 proteins, of which 522 were differentially expressed between B. terrestris and B. magnus. Several proteins with protective functions, such as proteases, antioxidant proteins and various heat-shock proteins, were present at higher levels in B. terrestris than in B. magnus under both control and heat-stress conditions. The same was true for proteins involved in cellular homeostasis, immunity, lipid/sugar metabolism and thermotolerance. Furthermore, proteins involved in the capture and elimination of reactive oxygen species also occurred at much high levels in B. terrestris. Overall, these results clearly indicate differences in the seminal proteome of the more thermotolerant B. terrestris versus B. magnus. The differences may contribute to explaining interspecific differences in sperm survival.https://royalsocietypublishing.org/doi/10.1098/rsos.231389bumblebeesglobal declinethermal toleranceheat exposureseminal fluidproteomics |
| spellingShingle | Baptiste Martinet Kimberly Przybyla Corentin Decroo Ruddy Wattiez Serge Aron Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance Royal Society Open Science bumblebees global decline thermal tolerance heat exposure seminal fluid proteomics |
| title | Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| title_full | Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| title_fullStr | Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| title_full_unstemmed | Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| title_short | Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| title_sort | proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance |
| topic | bumblebees global decline thermal tolerance heat exposure seminal fluid proteomics |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.231389 |
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