Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M
Background & objectives: The diagnosis of scrub typhus (ST) is usually done using enzyme-linked immunosorbent assay (ELISA) due to its ease of performance and reading objectivity. The cut-off value for ELISA needs to be calculated for each geographical location as it depends on zonal endemicity...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Indian Journal of Medical Research |
| Subjects: | |
| Online Access: | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2023;volume=157;issue=6;spage=519;epage=523;aulast=Jain |
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| author | Amita Jain Parul Jain Sanjay C Rebello Moulika Todaria Harmanmeet Kaur Nivedita Gupta Neeraj Aggarwal Neetu Vijay Jitendra Narayan |
| author_facet | Amita Jain Parul Jain Sanjay C Rebello Moulika Todaria Harmanmeet Kaur Nivedita Gupta Neeraj Aggarwal Neetu Vijay Jitendra Narayan |
| author_sort | Amita Jain |
| collection | DOAJ |
| description | Background & objectives: The diagnosis of scrub typhus (ST) is usually done using enzyme-linked immunosorbent assay (ELISA) due to its ease of performance and reading objectivity. The cut-off value for ELISA needs to be calculated for each geographical location as it depends on zonal endemicity of the disease. This study was, therefore, undertaken to calculate the pan-India cut-off for anti-Orientia tsutsugamushi (OT) immunoglobulin M (IgM) by ELISA.
Methods: Samples from cases (cases of ST) and controls (voluntary, consenting, healthy adults) were collected by a network of 29 laboratories across India and tested for anti-OT IgM by immunofluorescence assay (IFA), the considered gold standard test. These samples were retested by ELISA for anti-OT IgM and their optical densities (ODs) were used for cut-off estimation by receiver operating characteristic (ROC) curve.
Results: Anti-OT IgM ELISA ODs from 273 controls and 136 cases were used for the cut-off estimation. The ODs of the anti-OT IgM ELISA on healthy individuals and those of confirmed ST cases ranged from 0.1 to 0.75 and 0.5 to 4.718, respectively. ROC curve-based cut-off for ELISA was calculated as 0.554 at a sensitivity of 95.2 per cent and specificity of 95.1 per cent. A value of >1 was noted to have a specificity of 100 per cent in diagnosing ST.
Interpretation & conclusions: The cut-off calculated for India was similar to the previous cut-off that was used until now. |
| first_indexed | 2024-03-12T13:47:04Z |
| format | Article |
| id | doaj.art-5ef4445af68d4d179f9b570a497e4781 |
| institution | Directory Open Access Journal |
| issn | 0971-5916 |
| language | English |
| last_indexed | 2024-03-12T13:47:04Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Medical Research |
| spelling | doaj.art-5ef4445af68d4d179f9b570a497e47812023-08-23T08:18:01ZengWolters Kluwer Medknow PublicationsIndian Journal of Medical Research0971-59162023-01-01157651952310.4103/ijmr.IJMR_4098_20Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin MAmita JainParul JainSanjay C RebelloMoulika TodariaHarmanmeet KaurNivedita GuptaNeeraj AggarwalNeetu VijayJitendra NarayanBackground & objectives: The diagnosis of scrub typhus (ST) is usually done using enzyme-linked immunosorbent assay (ELISA) due to its ease of performance and reading objectivity. The cut-off value for ELISA needs to be calculated for each geographical location as it depends on zonal endemicity of the disease. This study was, therefore, undertaken to calculate the pan-India cut-off for anti-Orientia tsutsugamushi (OT) immunoglobulin M (IgM) by ELISA. Methods: Samples from cases (cases of ST) and controls (voluntary, consenting, healthy adults) were collected by a network of 29 laboratories across India and tested for anti-OT IgM by immunofluorescence assay (IFA), the considered gold standard test. These samples were retested by ELISA for anti-OT IgM and their optical densities (ODs) were used for cut-off estimation by receiver operating characteristic (ROC) curve. Results: Anti-OT IgM ELISA ODs from 273 controls and 136 cases were used for the cut-off estimation. The ODs of the anti-OT IgM ELISA on healthy individuals and those of confirmed ST cases ranged from 0.1 to 0.75 and 0.5 to 4.718, respectively. ROC curve-based cut-off for ELISA was calculated as 0.554 at a sensitivity of 95.2 per cent and specificity of 95.1 per cent. A value of >1 was noted to have a specificity of 100 per cent in diagnosing ST. Interpretation & conclusions: The cut-off calculated for India was similar to the previous cut-off that was used until now.http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2023;volume=157;issue=6;spage=519;epage=523;aulast=Jainenzyme-linked immunosorbent assay cut-off - indirect immunofluorescence assay - orientia tsutsugamushi - immunoglobulin m - scrub typhus |
| spellingShingle | Amita Jain Parul Jain Sanjay C Rebello Moulika Todaria Harmanmeet Kaur Nivedita Gupta Neeraj Aggarwal Neetu Vijay Jitendra Narayan Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M Indian Journal of Medical Research enzyme-linked immunosorbent assay cut-off - indirect immunofluorescence assay - orientia tsutsugamushi - immunoglobulin m - scrub typhus |
| title | Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M |
| title_full | Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M |
| title_fullStr | Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M |
| title_full_unstemmed | Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M |
| title_short | Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M |
| title_sort | determination of a cut off value for the serological diagnosis of scrub typhus by detecting anti orientia tsutsugamushi immunoglobulin m |
| topic | enzyme-linked immunosorbent assay cut-off - indirect immunofluorescence assay - orientia tsutsugamushi - immunoglobulin m - scrub typhus |
| url | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2023;volume=157;issue=6;spage=519;epage=523;aulast=Jain |
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