Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae
Saccharomyces cerevisiae is a widely used eukaryotic model and microbial cell factory. RNA interference (RNAi) is a conserved regulatory mechanism among eukaryotes but absent from S. cerevisiae. Recent reconstitution of RNAi machinery in S. cerevisiae enables the use of this powerful tool for strain...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00731/full |
| _version_ | 1819108434922962944 |
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| author | Yongcan Chen Erpeng Guo Jianzhi Zhang Tong Si |
| author_facet | Yongcan Chen Erpeng Guo Jianzhi Zhang Tong Si |
| author_sort | Yongcan Chen |
| collection | DOAJ |
| description | Saccharomyces cerevisiae is a widely used eukaryotic model and microbial cell factory. RNA interference (RNAi) is a conserved regulatory mechanism among eukaryotes but absent from S. cerevisiae. Recent reconstitution of RNAi machinery in S. cerevisiae enables the use of this powerful tool for strain engineering. Here we first discuss the introduction of heterologous RNAi pathways in S. cerevisiae, and the design of various expression cassettes of RNAi precursor reagents for tunable, dynamic, and genome-wide regulation. We then summarize notable examples of RNAi-assisted functional genomics and metabolic engineering studies in S. cerevisiae. We conclude with the future challenges and opportunities of RNAi-based approaches, as well as the potential of other regulatory RNAs in advancing yeast engineering. |
| first_indexed | 2024-12-22T03:09:53Z |
| format | Article |
| id | doaj.art-5ef85bc3bad145a49fcc0d1c1f9363bc |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T03:09:53Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-5ef85bc3bad145a49fcc0d1c1f9363bc2022-12-21T18:40:57ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-07-01810.3389/fbioe.2020.00731553001Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiaeYongcan ChenErpeng GuoJianzhi ZhangTong SiSaccharomyces cerevisiae is a widely used eukaryotic model and microbial cell factory. RNA interference (RNAi) is a conserved regulatory mechanism among eukaryotes but absent from S. cerevisiae. Recent reconstitution of RNAi machinery in S. cerevisiae enables the use of this powerful tool for strain engineering. Here we first discuss the introduction of heterologous RNAi pathways in S. cerevisiae, and the design of various expression cassettes of RNAi precursor reagents for tunable, dynamic, and genome-wide regulation. We then summarize notable examples of RNAi-assisted functional genomics and metabolic engineering studies in S. cerevisiae. We conclude with the future challenges and opportunities of RNAi-based approaches, as well as the potential of other regulatory RNAs in advancing yeast engineering.https://www.frontiersin.org/article/10.3389/fbioe.2020.00731/fullRNAiSaccharomyces cerevisiaestrain engineeringfunctional genomicssynthetic biology |
| spellingShingle | Yongcan Chen Erpeng Guo Jianzhi Zhang Tong Si Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae Frontiers in Bioengineering and Biotechnology RNAi Saccharomyces cerevisiae strain engineering functional genomics synthetic biology |
| title | Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae |
| title_full | Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae |
| title_fullStr | Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae |
| title_full_unstemmed | Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae |
| title_short | Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae |
| title_sort | advances in rnai assisted strain engineering in saccharomyces cerevisiae |
| topic | RNAi Saccharomyces cerevisiae strain engineering functional genomics synthetic biology |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00731/full |
| work_keys_str_mv | AT yongcanchen advancesinrnaiassistedstrainengineeringinsaccharomycescerevisiae AT erpengguo advancesinrnaiassistedstrainengineeringinsaccharomycescerevisiae AT jianzhizhang advancesinrnaiassistedstrainengineeringinsaccharomycescerevisiae AT tongsi advancesinrnaiassistedstrainengineeringinsaccharomycescerevisiae |