How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics
Polymeric microparticles (MPs) designed for the intravitreal administration of therapeutic proteins result in a prolonged half-life in the vitreous and can delay or discourage the onset of adverse effects inevitably related to this route of administration. Hence, here we designed MPs composed of a p...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/16/7567 |
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| author | Teresa Silvestri Barbara Immirzi Giovanni Dal Poggetto Paola Di Donato Valentina Mollo Laura Mayol Marco Biondi |
| author_facet | Teresa Silvestri Barbara Immirzi Giovanni Dal Poggetto Paola Di Donato Valentina Mollo Laura Mayol Marco Biondi |
| author_sort | Teresa Silvestri |
| collection | DOAJ |
| description | Polymeric microparticles (MPs) designed for the intravitreal administration of therapeutic proteins result in a prolonged half-life in the vitreous and can delay or discourage the onset of adverse effects inevitably related to this route of administration. Hence, here we designed MPs composed of a polymeric blend based on poly(lactic-co-glycolic) acid and poloxamers, externally decorated with hyaluronic acid. The MPs are intended for intravitreal administration of bovine serum albumin. In detail, a systematic formulative study aiming to shed light on the complex relationship between protein release rate and MP degradation rate was carried out by means of calorimetric and gel permeation chromatography analyses. We found out that poloxamer addition caused a compact MP matrix, which led to a slight modification of the degradation kinetics and a reduction in the initial BSA initial release, which is of the utmost importance to ensure a relatively regular BSA release. It must also be underlined that for acid-labile molecules such as proteins, the poloxamer’s presence induced complex and hardly predictable effects on MP degradation/protein release, due to the dynamic balance between the time-evolving hydrophilicity of MPs and the influence of poloxamers themselves on the PLGA degradation rate. |
| first_indexed | 2024-03-10T09:01:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T09:01:20Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-5ef9326e2eba4731a8ea53844226ee412023-11-22T06:43:35ZengMDPI AGApplied Sciences2076-34172021-08-011116756710.3390/app11167567How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release KineticsTeresa Silvestri0Barbara Immirzi1Giovanni Dal Poggetto2Paola Di Donato3Valentina Mollo4Laura Mayol5Marco Biondi6Scuola di Medicina e Chirurgia, Università di Napoli Federico II, Via Domenico Montesano 49, 80131 Napoli, ItalyInstitute of Polymers, Composites and Biomaterials (IPCB), C/o Comprensorio Olivetti, Via Campi Flegrei 34, 80078 Pozzuoli, ItalyInstitute of Polymers, Composites and Biomaterials (IPCB), C/o Comprensorio Olivetti, Via Campi Flegrei 34, 80078 Pozzuoli, ItalyDepartment of Science and Technology, University of Naples Parthenope, Centro Direzionale, Isola C4, 80143 Naples, ItalyItalian Institute of Technology@CRIB Center for Advanced Biomaterials for Health Care, 80125 Napoli, ItalyScuola di Medicina e Chirurgia, Università di Napoli Federico II, Via Domenico Montesano 49, 80131 Napoli, ItalyScuola di Medicina e Chirurgia, Università di Napoli Federico II, Via Domenico Montesano 49, 80131 Napoli, ItalyPolymeric microparticles (MPs) designed for the intravitreal administration of therapeutic proteins result in a prolonged half-life in the vitreous and can delay or discourage the onset of adverse effects inevitably related to this route of administration. Hence, here we designed MPs composed of a polymeric blend based on poly(lactic-co-glycolic) acid and poloxamers, externally decorated with hyaluronic acid. The MPs are intended for intravitreal administration of bovine serum albumin. In detail, a systematic formulative study aiming to shed light on the complex relationship between protein release rate and MP degradation rate was carried out by means of calorimetric and gel permeation chromatography analyses. We found out that poloxamer addition caused a compact MP matrix, which led to a slight modification of the degradation kinetics and a reduction in the initial BSA initial release, which is of the utmost importance to ensure a relatively regular BSA release. It must also be underlined that for acid-labile molecules such as proteins, the poloxamer’s presence induced complex and hardly predictable effects on MP degradation/protein release, due to the dynamic balance between the time-evolving hydrophilicity of MPs and the influence of poloxamers themselves on the PLGA degradation rate.https://www.mdpi.com/2076-3417/11/16/7567biodegradablemicroparticlesocular drug deliveryPLGAhyaluronic acidpoloxamers |
| spellingShingle | Teresa Silvestri Barbara Immirzi Giovanni Dal Poggetto Paola Di Donato Valentina Mollo Laura Mayol Marco Biondi How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics Applied Sciences biodegradable microparticles ocular drug delivery PLGA hyaluronic acid poloxamers |
| title | How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics |
| title_full | How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics |
| title_fullStr | How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics |
| title_full_unstemmed | How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics |
| title_short | How Poloxamer Addition in Hyaluronic-Acid-Decorated Biodegradable Microparticles Affects Polymer Degradation and Protein Release Kinetics |
| title_sort | how poloxamer addition in hyaluronic acid decorated biodegradable microparticles affects polymer degradation and protein release kinetics |
| topic | biodegradable microparticles ocular drug delivery PLGA hyaluronic acid poloxamers |
| url | https://www.mdpi.com/2076-3417/11/16/7567 |
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