Trypanosoma cruzi survival following cold storage: possible implications for tissue banking.
While Trypanosoma cruzi, the etiologic agent of Chagas disease, is typically vector-borne, infection can also occur through solid organ transplantation or transfusion of contaminated blood products. The ability of infected human cells, tissues, and cellular and tissue-based products (HCT/Ps) to tran...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095398&type=printable |
| _version_ | 1826587041610072064 |
|---|---|
| author | Diana L Martin Brook Goodhew Nancy Czaicki Kawanda Foster Srijana Rajbhandary Shawn Hunter Scott A Brubaker |
| author_facet | Diana L Martin Brook Goodhew Nancy Czaicki Kawanda Foster Srijana Rajbhandary Shawn Hunter Scott A Brubaker |
| author_sort | Diana L Martin |
| collection | DOAJ |
| description | While Trypanosoma cruzi, the etiologic agent of Chagas disease, is typically vector-borne, infection can also occur through solid organ transplantation or transfusion of contaminated blood products. The ability of infected human cells, tissues, and cellular and tissue-based products (HCT/Ps) to transmit T. cruzi is dependent upon T. cruzi surviving the processing and storage conditions to which HCT/Ps are subjected. In the studies reported here, T. cruzi trypomastigotes remained infective 24 hours after being spiked into blood and stored at room temperature (N = 20); in 2 of 13 parasite-infected cultures stored 28 days at 4°C; and in samples stored 365 days at -80°C without cryoprotectant (N = 28), despite decreased viability compared to cryopreserved parasites. Detection of viable parasites after multiple freeze/thaws depended upon the duration of frozen storage. The ability of T. cruzi to survive long periods of storage at +4 and -80°C suggests that T. cruzi-infected tissues stored under these conditions are potentially infectious. |
| first_indexed | 2024-12-11T20:36:39Z |
| format | Article |
| id | doaj.art-5efb601fa00a4d00b8ecdd369ad53950 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T16:19:08Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5efb601fa00a4d00b8ecdd369ad539502025-02-22T05:34:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9539810.1371/journal.pone.0095398Trypanosoma cruzi survival following cold storage: possible implications for tissue banking.Diana L MartinBrook GoodhewNancy CzaickiKawanda FosterSrijana RajbhandaryShawn HunterScott A BrubakerWhile Trypanosoma cruzi, the etiologic agent of Chagas disease, is typically vector-borne, infection can also occur through solid organ transplantation or transfusion of contaminated blood products. The ability of infected human cells, tissues, and cellular and tissue-based products (HCT/Ps) to transmit T. cruzi is dependent upon T. cruzi surviving the processing and storage conditions to which HCT/Ps are subjected. In the studies reported here, T. cruzi trypomastigotes remained infective 24 hours after being spiked into blood and stored at room temperature (N = 20); in 2 of 13 parasite-infected cultures stored 28 days at 4°C; and in samples stored 365 days at -80°C without cryoprotectant (N = 28), despite decreased viability compared to cryopreserved parasites. Detection of viable parasites after multiple freeze/thaws depended upon the duration of frozen storage. The ability of T. cruzi to survive long periods of storage at +4 and -80°C suggests that T. cruzi-infected tissues stored under these conditions are potentially infectious.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095398&type=printable |
| spellingShingle | Diana L Martin Brook Goodhew Nancy Czaicki Kawanda Foster Srijana Rajbhandary Shawn Hunter Scott A Brubaker Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. PLoS ONE |
| title | Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. |
| title_full | Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. |
| title_fullStr | Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. |
| title_full_unstemmed | Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. |
| title_short | Trypanosoma cruzi survival following cold storage: possible implications for tissue banking. |
| title_sort | trypanosoma cruzi survival following cold storage possible implications for tissue banking |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095398&type=printable |
| work_keys_str_mv | AT dianalmartin trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT brookgoodhew trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT nancyczaicki trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT kawandafoster trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT srijanarajbhandary trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT shawnhunter trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking AT scottabrubaker trypanosomacruzisurvivalfollowingcoldstoragepossibleimplicationsfortissuebanking |