Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats
Objective: To explore the combinatorial effect of epigallocatechin-3-gallate (EGCG) and calorie restriction on activation of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor involved in the antioxidant defense system of aged rats. Methods: Aged male Wistar rats were calorie...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=10;spage=421;epage=430;aulast=Ravindran |
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| author | Rajeswari Ravindran Malathi Manuel Thangarajeswari Mohan Ravindran Jaganathan Kalaiselvi Periandavan |
| author_facet | Rajeswari Ravindran Malathi Manuel Thangarajeswari Mohan Ravindran Jaganathan Kalaiselvi Periandavan |
| author_sort | Rajeswari Ravindran |
| collection | DOAJ |
| description | Objective: To explore the combinatorial effect of epigallocatechin-3-gallate (EGCG) and calorie restriction on activation of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor involved in the antioxidant defense system of aged rats.
Methods: Aged male Wistar rats were calorie-restricted and treated with EGCG orally for 45 days. The initial body weight of aged rats was recorded, and the final body weight was measured at the end of the experimental period. Serum lipid and lipoprotein status, oxidative stress markers such as free radicals and malondialdehyde levels, and reduced glutathione were assessed. In addition, RT-PCR and Western blotting analyses were performed.
Results: Calorie restriction potentiated the effect of EGCG on enhancing antioxidant status, improving the levels of serum lipid and lipoproteins, upregulating Nrf2 and Bcl2, and downregulating Keap1, cullin3, Bax and cytochrome c in aged rats.
Conclusions: Calorie restriction can promote EGCG-mediated Nrf2 activation in aged rats. This preliminary finding paves the way for a combinatory approach to replenishing the antioxidant status during aging, thereby reducing the risk for age-associated degenerative diseases. |
| first_indexed | 2024-03-08T13:13:14Z |
| format | Article |
| id | doaj.art-5eff5a6477014834b1a8ca9ebfc4458a |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2025-03-21T22:01:26Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-5eff5a6477014834b1a8ca9ebfc4458a2024-05-25T11:46:41ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222023-01-01131042143010.4103/2221-1691.387748Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged ratsRajeswari RavindranMalathi ManuelThangarajeswari MohanRavindran JaganathanKalaiselvi PeriandavanObjective: To explore the combinatorial effect of epigallocatechin-3-gallate (EGCG) and calorie restriction on activation of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor involved in the antioxidant defense system of aged rats. Methods: Aged male Wistar rats were calorie-restricted and treated with EGCG orally for 45 days. The initial body weight of aged rats was recorded, and the final body weight was measured at the end of the experimental period. Serum lipid and lipoprotein status, oxidative stress markers such as free radicals and malondialdehyde levels, and reduced glutathione were assessed. In addition, RT-PCR and Western blotting analyses were performed. Results: Calorie restriction potentiated the effect of EGCG on enhancing antioxidant status, improving the levels of serum lipid and lipoproteins, upregulating Nrf2 and Bcl2, and downregulating Keap1, cullin3, Bax and cytochrome c in aged rats. Conclusions: Calorie restriction can promote EGCG-mediated Nrf2 activation in aged rats. This preliminary finding paves the way for a combinatory approach to replenishing the antioxidant status during aging, thereby reducing the risk for age-associated degenerative diseases.http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=10;spage=421;epage=430;aulast=Ravindranaging; reactive oxygen species; calorie restriction; egcg; nrf2; antioxidant |
| spellingShingle | Rajeswari Ravindran Malathi Manuel Thangarajeswari Mohan Ravindran Jaganathan Kalaiselvi Periandavan Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats Asian Pacific Journal of Tropical Biomedicine aging; reactive oxygen species; calorie restriction; egcg; nrf2; antioxidant |
| title | Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats |
| title_full | Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats |
| title_fullStr | Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats |
| title_full_unstemmed | Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats |
| title_short | Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats |
| title_sort | calorie restriction potentiates epigallocatechin 3 gallate mediated nrf2 activation in hepatocytes of aged rats |
| topic | aging; reactive oxygen species; calorie restriction; egcg; nrf2; antioxidant |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=10;spage=421;epage=430;aulast=Ravindran |
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