Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass
Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-06-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_4358_Lee_Optimization_Components_Enzymatic_Hydrolysis |
| _version_ | 1819065705500246016 |
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| author | Young Min Lee Hanbyul Lee Jun Seok Kim Jaejung Lee Byoung Jun Ahn Gyu-Hyeok Kim Jae-Jin Kim |
| author_facet | Young Min Lee Hanbyul Lee Jun Seok Kim Jaejung Lee Byoung Jun Ahn Gyu-Hyeok Kim Jae-Jin Kim |
| author_sort | Young Min Lee |
| collection | DOAJ |
| description | Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase. |
| first_indexed | 2024-12-21T15:50:43Z |
| format | Article |
| id | doaj.art-5eff7101c76e4ea6961b0773fbff5d5a |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-21T15:50:43Z |
| publishDate | 2014-06-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-5eff7101c76e4ea6961b0773fbff5d5a2022-12-21T18:58:14ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-06-01934358436810.15376/biores.9.3.4358-4368Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic BiomassYoung Min Lee0Hanbyul Lee1Jun Seok Kim2Jaejung Lee3Byoung Jun Ahn4Gyu-Hyeok Kim5Jae-Jin Kim6Korea UniversityKorea UniversityKyonggi UniversityDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701 KoreaDivision of Wood Chemistry and Microbiology, Korea Forest Research Institute, 57-Hoegiro, Dongdaemun-gu, Seoul, 130-712 KoreaKorea UniversityKorea UniversityMedium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_4358_Lee_Optimization_Components_Enzymatic_Hydrolysisβ-glucosidaseOptimizationSaccharificationSchizophyllum commune |
| spellingShingle | Young Min Lee Hanbyul Lee Jun Seok Kim Jaejung Lee Byoung Jun Ahn Gyu-Hyeok Kim Jae-Jin Kim Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass BioResources β-glucosidase Optimization Saccharification Schizophyllum commune |
| title | Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass |
| title_full | Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass |
| title_fullStr | Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass |
| title_full_unstemmed | Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass |
| title_short | Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass |
| title_sort | optimization of medium components for β glucosidase production in schizophyllum commune kuc9397 and enzymatic hydrolysis of lignocellulosic biomass |
| topic | β-glucosidase Optimization Saccharification Schizophyllum commune |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_4358_Lee_Optimization_Components_Enzymatic_Hydrolysis |
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