Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i>
OVATE family proteins (OFPs) are a kind of plant-specific transcription factor, which play important roles in the growth and development of plants. Here, we performed a genome-wide investigation of the <i>OFP</i> gene family members in the wild diploid strawberry (<i>Fragaria vesca...
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MDPI AG
2024-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/3/569 |
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| author | Xiao Xu Xinyu Wang Sirui Zhou Xumo Huang Pengcheng Liu Bojun Ma Xifeng Chen |
| author_facet | Xiao Xu Xinyu Wang Sirui Zhou Xumo Huang Pengcheng Liu Bojun Ma Xifeng Chen |
| author_sort | Xiao Xu |
| collection | DOAJ |
| description | OVATE family proteins (OFPs) are a kind of plant-specific transcription factor, which play important roles in the growth and development of plants. Here, we performed a genome-wide investigation of the <i>OFP</i> gene family members in the wild diploid strawberry (<i>Fragaria vesca</i>, 2<i>n</i> = 14), and analyzed their physical and chemical properties, gene structure, phylogeny, expression patterns, and the subcellular localizations of these genes. Fourteen <i>OFP</i> genes from <i>F.vesca</i> were identified. Collinearity analysis showed ten pairs of collinearity between <i>F. vesca</i> and Arabidopsis. Phylogenetic analysis divided <i>FvOFP</i> genes into five different clades. The expression patterns of the <i>FvOFP</i> genes assayed in different tissues of <i>F. vesca</i> by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) showed that <i>FvOFP1</i>, <i>FvOFP11</i>, <i>FvOFP12</i>, and <i>FvOFP14</i> were highly expressed in achene and their expression was further verified in the fruits at different developmental stages. Additionally, the subcellular localizations of FvOFP1, FvOFP11, FvOFP12, and FvOFP14 were preliminarily analyzed in tobacco leaves. The results showed clear fluorescent signals in the nucleus. Our results provided a comprehensive understanding of the potential function of <i>FvOFP</i> genes in strawberries. |
| first_indexed | 2024-04-24T18:38:46Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-04-24T18:38:46Z |
| publishDate | 2024-03-01 |
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| series | Agronomy |
| spelling | doaj.art-5f0083bc58554fb0bb362143d28ba35a2024-03-27T13:16:55ZengMDPI AGAgronomy2073-43952024-03-0114356910.3390/agronomy14030569Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i>Xiao Xu0Xinyu Wang1Sirui Zhou2Xumo Huang3Pengcheng Liu4Bojun Ma5Xifeng Chen6College of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaOVATE family proteins (OFPs) are a kind of plant-specific transcription factor, which play important roles in the growth and development of plants. Here, we performed a genome-wide investigation of the <i>OFP</i> gene family members in the wild diploid strawberry (<i>Fragaria vesca</i>, 2<i>n</i> = 14), and analyzed their physical and chemical properties, gene structure, phylogeny, expression patterns, and the subcellular localizations of these genes. Fourteen <i>OFP</i> genes from <i>F.vesca</i> were identified. Collinearity analysis showed ten pairs of collinearity between <i>F. vesca</i> and Arabidopsis. Phylogenetic analysis divided <i>FvOFP</i> genes into five different clades. The expression patterns of the <i>FvOFP</i> genes assayed in different tissues of <i>F. vesca</i> by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) showed that <i>FvOFP1</i>, <i>FvOFP11</i>, <i>FvOFP12</i>, and <i>FvOFP14</i> were highly expressed in achene and their expression was further verified in the fruits at different developmental stages. Additionally, the subcellular localizations of FvOFP1, FvOFP11, FvOFP12, and FvOFP14 were preliminarily analyzed in tobacco leaves. The results showed clear fluorescent signals in the nucleus. Our results provided a comprehensive understanding of the potential function of <i>FvOFP</i> genes in strawberries.https://www.mdpi.com/2073-4395/14/3/569<i>OFP</i><i>Fragaria vesca</i>gene identificationexpression patternsubcellular localization |
| spellingShingle | Xiao Xu Xinyu Wang Sirui Zhou Xumo Huang Pengcheng Liu Bojun Ma Xifeng Chen Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> Agronomy <i>OFP</i> <i>Fragaria vesca</i> gene identification expression pattern subcellular localization |
| title | Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> |
| title_full | Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> |
| title_fullStr | Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> |
| title_full_unstemmed | Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> |
| title_short | Genome-Wide Identification and Characterization of the <i>OFP</i> Gene Family in the Wild Strawberry <i>Fragaria vesca</i> |
| title_sort | genome wide identification and characterization of the i ofp i gene family in the wild strawberry i fragaria vesca i |
| topic | <i>OFP</i> <i>Fragaria vesca</i> gene identification expression pattern subcellular localization |
| url | https://www.mdpi.com/2073-4395/14/3/569 |
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