Study on Analytical Method of Anthocyanin in Health Food
In order to establish a method for the determination of anthocyanins in health foods, thin-layer chromatography (TLC) was used for qualitative identification of anthocyanins, and high-performance liquid chromatography (HPLC) was used for quantitative detection of anthocyanins. The results showed tha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of National Food and Strategic Reserves Administration
2022-01-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220124?st=article_issue |
| _version_ | 1797842838114795520 |
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| author | BAI Wei-rong ZHANG Shu-jia GUO Xiao-li GAO Ru-yi |
| author_facet | BAI Wei-rong ZHANG Shu-jia GUO Xiao-li GAO Ru-yi |
| author_sort | BAI Wei-rong |
| collection | DOAJ |
| description | In order to establish a method for the determination of anthocyanins in health foods, thin-layer chromatography (TLC) was used for qualitative identification of anthocyanins, and high-performance liquid chromatography (HPLC) was used for quantitative detection of anthocyanins. The results showed that the suitable conditions for TLC identification were developing agent: V(formic acid)∶V(water-n)∶V(butanol)= 9.1∶8.4∶32.5, spotting amount: 2 μL. Anthocyanin was detected at 4.361~52.332 μg/mL (R2 =0.999 5). The internal linear relationship is good, with the average sample recovery rate is 98.22% (RSD=1.23%, n=6), and the precision, repeatability and stability are good. Experiments show that the established thin-layer identification method for anthocyanins is stable and feasible. |
| first_indexed | 2024-04-09T16:54:44Z |
| format | Article |
| id | doaj.art-5f0a0543325b4c459c2e919fe42594d8 |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-09T16:54:44Z |
| publishDate | 2022-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-5f0a0543325b4c459c2e919fe42594d82023-04-21T05:57:35ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612022-01-0130115015610.16210/j.cnki.1007-7561.2022.01.018Study on Analytical Method of Anthocyanin in Health FoodBAI Wei-rong0ZHANG Shu-jia1GUO Xiao-li2GAO Ru-yi3Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222001, ChinaJiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222001, ChinaJiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222001, ChinaJiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222001, ChinaIn order to establish a method for the determination of anthocyanins in health foods, thin-layer chromatography (TLC) was used for qualitative identification of anthocyanins, and high-performance liquid chromatography (HPLC) was used for quantitative detection of anthocyanins. The results showed that the suitable conditions for TLC identification were developing agent: V(formic acid)∶V(water-n)∶V(butanol)= 9.1∶8.4∶32.5, spotting amount: 2 μL. Anthocyanin was detected at 4.361~52.332 μg/mL (R2 =0.999 5). The internal linear relationship is good, with the average sample recovery rate is 98.22% (RSD=1.23%, n=6), and the precision, repeatability and stability are good. Experiments show that the established thin-layer identification method for anthocyanins is stable and feasible.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220124?st=article_issuehealth foodsrelieving visual fatigueanthocyaninsthin layer chromatography (tlc)high performance liquid chromatography (hplc) |
| spellingShingle | BAI Wei-rong ZHANG Shu-jia GUO Xiao-li GAO Ru-yi Study on Analytical Method of Anthocyanin in Health Food Liang you shipin ke-ji health foods relieving visual fatigue anthocyanins thin layer chromatography (tlc) high performance liquid chromatography (hplc) |
| title | Study on Analytical Method of Anthocyanin in Health Food |
| title_full | Study on Analytical Method of Anthocyanin in Health Food |
| title_fullStr | Study on Analytical Method of Anthocyanin in Health Food |
| title_full_unstemmed | Study on Analytical Method of Anthocyanin in Health Food |
| title_short | Study on Analytical Method of Anthocyanin in Health Food |
| title_sort | study on analytical method of anthocyanin in health food |
| topic | health foods relieving visual fatigue anthocyanins thin layer chromatography (tlc) high performance liquid chromatography (hplc) |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220124?st=article_issue |
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