Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats
AIM:To investigate the expression and mechanism of high mobility group box protein-1(HMGB1)in the retina of diabetic rats. <p>METHODS:Sixty SD rats were randomly divided into diabetic group and control group. Diabetic rat model was produced by intraperitioneal injection of 1% STZ with 60mg/Kg...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2016-05-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2016/5/201605009.pdf |
| _version_ | 1818518617540526080 |
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| author | Shuang Jiang Hai-Yue Xu |
| author_facet | Shuang Jiang Hai-Yue Xu |
| author_sort | Shuang Jiang |
| collection | DOAJ |
| description | AIM:To investigate the expression and mechanism of high mobility group box protein-1(HMGB1)in the retina of diabetic rats. <p>METHODS:Sixty SD rats were randomly divided into diabetic group and control group. Diabetic rat model was produced by intraperitioneal injection of 1% STZ with 60mg/Kg weight. The rats in control group received intraperitioneal injection of normal saline with same dosage. After injection, the rats were sacrificed and eyeballs were enucleated for HE staining, the retina fluorescence angiography, TUNEL and Western Blot detection at 1, 2 and 4mo for the expressions of HMGB1 and NF-κB. <p>RESULTS:Compared with the control group, the retinal cells disorder, cell densities decreases, microvasculars occlusion were founded with inner and outer nuclear layer thinning and ganglion cell apoptosis. The fluorescence angiography showed that peripheral capillaries became circuitous and vascular occlusion and non-perfusion area could be seen. The expressions of HMGB1 and NF-κB were higher than those of control with time dependence and they had significant positive correlations(<i>P</i><0.05).<p>CONCLUSION:The expression of HMGB1 increases in diabetic rat retina, which may involve in the occurrence of diabetic retinopathy through the NF- κB pathway. |
| first_indexed | 2024-12-11T01:12:37Z |
| format | Article |
| id | doaj.art-5f0bed8abc6a44779f06c0296b65b0e5 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-11T01:12:37Z |
| publishDate | 2016-05-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-5f0bed8abc6a44779f06c0296b65b0e52022-12-22T01:25:59ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232016-05-0116582983210.3980/j.issn.1672-5123.2016.5.09Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic ratsShuang Jiang0Hai-Yue Xu1Department of Ophthalmology, the Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, ChinaDepartment of Ophthalmology, the Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, ChinaAIM:To investigate the expression and mechanism of high mobility group box protein-1(HMGB1)in the retina of diabetic rats. <p>METHODS:Sixty SD rats were randomly divided into diabetic group and control group. Diabetic rat model was produced by intraperitioneal injection of 1% STZ with 60mg/Kg weight. The rats in control group received intraperitioneal injection of normal saline with same dosage. After injection, the rats were sacrificed and eyeballs were enucleated for HE staining, the retina fluorescence angiography, TUNEL and Western Blot detection at 1, 2 and 4mo for the expressions of HMGB1 and NF-κB. <p>RESULTS:Compared with the control group, the retinal cells disorder, cell densities decreases, microvasculars occlusion were founded with inner and outer nuclear layer thinning and ganglion cell apoptosis. The fluorescence angiography showed that peripheral capillaries became circuitous and vascular occlusion and non-perfusion area could be seen. The expressions of HMGB1 and NF-κB were higher than those of control with time dependence and they had significant positive correlations(<i>P</i><0.05).<p>CONCLUSION:The expression of HMGB1 increases in diabetic rat retina, which may involve in the occurrence of diabetic retinopathy through the NF- κB pathway.http://ies.ijo.cn/cn_publish/2016/5/201605009.pdfdiabetic retinopathyhigh mobility group box protein-1NF-Κb |
| spellingShingle | Shuang Jiang Hai-Yue Xu Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats Guoji Yanke Zazhi diabetic retinopathy high mobility group box protein-1 NF-Κb |
| title | Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats |
| title_full | Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats |
| title_fullStr | Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats |
| title_full_unstemmed | Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats |
| title_short | Expression and mechanism of high mobility group box protein-1 in retinal tissue of diabetic rats |
| title_sort | expression and mechanism of high mobility group box protein 1 in retinal tissue of diabetic rats |
| topic | diabetic retinopathy high mobility group box protein-1 NF-Κb |
| url | http://ies.ijo.cn/cn_publish/2016/5/201605009.pdf |
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