Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis
Abstract Surface assisted laser desorption ionization mass spectrometry (SALDI‐MS) is a powerful analytical technology for the high‐throughput detection of metabolites, drugs, and other bioactive molecules. The performance of SALDI‐MS in enhancing molecules desorption and ionization mainly depends o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2021-12-01
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| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202101157 |
| _version_ | 1797763908258234368 |
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| author | Wenya Chang Xueliang Ouyang Huan Zhong Daoxue Liu Dawei Deng Zhuoying Xie |
| author_facet | Wenya Chang Xueliang Ouyang Huan Zhong Daoxue Liu Dawei Deng Zhuoying Xie |
| author_sort | Wenya Chang |
| collection | DOAJ |
| description | Abstract Surface assisted laser desorption ionization mass spectrometry (SALDI‐MS) is a powerful analytical technology for the high‐throughput detection of metabolites, drugs, and other bioactive molecules. The performance of SALDI‐MS in enhancing molecules desorption and ionization mainly depends on the surface properties of matrix utilized. Here, black titanium dioxide (B‐TiO2) nanoparticle is used for the first time as a novel matrix for enhancing laser desorption and ionization (LDI) and demonstrates significant advantages over white titanium dioxide (W‐TiO2). Further, the black mesoporous TiO2 coated Fe3O4 magnetic nanoparticles (B‐mTiO2 MNPs) are designed and prepared, which possess a high molar absorption coefficient, strong magnetic response, higher specific surface area, and ample phosphorylation binding sites. The B‐mTiO2 MNPs exhibit a superior ionization efficiency, low background signal, and good reproducibility (RSD < 10%) in the analysis of compounds with different molecular weights and practical bio‐sample. Salt tolerance of B‐mTiO2 MNPs up to 10 mm PBS, meanwhile, B‐mTiO2 MNPs also provides excellent enrichment efficiency for phosphopeptides (the detection limit low to 5 × 10−10 M). |
| first_indexed | 2024-03-12T19:48:07Z |
| format | Article |
| id | doaj.art-5f0dfd38f6a34b65b388c688e9fc28ea |
| institution | Directory Open Access Journal |
| issn | 2196-7350 |
| language | English |
| last_indexed | 2024-03-12T19:48:07Z |
| publishDate | 2021-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj.art-5f0dfd38f6a34b65b388c688e9fc28ea2023-08-02T03:24:30ZengWiley-VCHAdvanced Materials Interfaces2196-73502021-12-01824n/an/a10.1002/admi.202101157Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum AnalysisWenya Chang0Xueliang Ouyang1Huan Zhong2Daoxue Liu3Dawei Deng4Zhuoying Xie5Department of Pharmaceutical Engineering School of Engineering China Pharmaceutical University Nanjing 211198 ChinaDepartment of Pharmaceutical Engineering School of Engineering China Pharmaceutical University Nanjing 211198 ChinaState Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 ChinaState Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 ChinaDepartment of Pharmaceutical Engineering School of Engineering China Pharmaceutical University Nanjing 211198 ChinaState Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 ChinaAbstract Surface assisted laser desorption ionization mass spectrometry (SALDI‐MS) is a powerful analytical technology for the high‐throughput detection of metabolites, drugs, and other bioactive molecules. The performance of SALDI‐MS in enhancing molecules desorption and ionization mainly depends on the surface properties of matrix utilized. Here, black titanium dioxide (B‐TiO2) nanoparticle is used for the first time as a novel matrix for enhancing laser desorption and ionization (LDI) and demonstrates significant advantages over white titanium dioxide (W‐TiO2). Further, the black mesoporous TiO2 coated Fe3O4 magnetic nanoparticles (B‐mTiO2 MNPs) are designed and prepared, which possess a high molar absorption coefficient, strong magnetic response, higher specific surface area, and ample phosphorylation binding sites. The B‐mTiO2 MNPs exhibit a superior ionization efficiency, low background signal, and good reproducibility (RSD < 10%) in the analysis of compounds with different molecular weights and practical bio‐sample. Salt tolerance of B‐mTiO2 MNPs up to 10 mm PBS, meanwhile, B‐mTiO2 MNPs also provides excellent enrichment efficiency for phosphopeptides (the detection limit low to 5 × 10−10 M).https://doi.org/10.1002/admi.202101157bioactive small moleculesblack TiO 2enrichment phosphopeptideslaser desorption, laser ionizationmesoporous nanoparticles |
| spellingShingle | Wenya Chang Xueliang Ouyang Huan Zhong Daoxue Liu Dawei Deng Zhuoying Xie Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis Advanced Materials Interfaces bioactive small molecules black TiO 2 enrichment phosphopeptides laser desorption, laser ionization mesoporous nanoparticles |
| title | Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis |
| title_full | Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis |
| title_fullStr | Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis |
| title_full_unstemmed | Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis |
| title_short | Black Mesoporous TiO2 Nanoparticles for Enhancing Surface Assisted Laser Desorption and Ionization in Mass Spectrum Analysis |
| title_sort | black mesoporous tio2 nanoparticles for enhancing surface assisted laser desorption and ionization in mass spectrum analysis |
| topic | bioactive small molecules black TiO 2 enrichment phosphopeptides laser desorption, laser ionization mesoporous nanoparticles |
| url | https://doi.org/10.1002/admi.202101157 |
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