The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease
Molecular hydrogen (H<sub>2</sub>) has been recognized as a novel medical gas with antioxidant and anti-inflammatory effects. Non-alcoholic fatty liver disease (NAFLD) is a liver pathology with increased fat accumulation in liver tissue caused by factors other than alcohol consumption. P...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12477 |
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| author | Zuzana Sumbalová Jarmila Kucharská Zuzana Rausová Anna Gvozdjáková Mária Szántová Branislav Kura Viliam Mojto Ján Slezák |
| author_facet | Zuzana Sumbalová Jarmila Kucharská Zuzana Rausová Anna Gvozdjáková Mária Szántová Branislav Kura Viliam Mojto Ján Slezák |
| author_sort | Zuzana Sumbalová |
| collection | DOAJ |
| description | Molecular hydrogen (H<sub>2</sub>) has been recognized as a novel medical gas with antioxidant and anti-inflammatory effects. Non-alcoholic fatty liver disease (NAFLD) is a liver pathology with increased fat accumulation in liver tissue caused by factors other than alcohol consumption. Platelet mitochondrial function is considered to reflect systemic mitochondrial health. We studied the effect of adjuvant therapy with hydrogen-rich water (HRW) on coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) content and platelet mitochondrial bioenergetics in patients with NAFLD. A total of 30 patients with NAFLD and 15 healthy volunteers were included in this clinical trial. A total of 17 patients (H<sub>2</sub> group) drank water three × 330 mL/day with tablets producing HRW (>4 mg/L H<sub>2</sub>) for 8 weeks, and 13 patients (P group) drank water with placebo tablets producing CO<sub>2</sub>. The concentration of CoQ<sub>10-TOTAL</sub> was determined by the HPLC method, the parameter of oxidative stress, thiobarbituric acid reactive substances (TBARS), by the spectrophotometric method, and mitochondrial bioenergetics in platelets isolated from whole blood by high-resolution respirometry. The patients with NAFLD had lower concentrations of CoQ<sub>10-TOTAL</sub> in the blood, plasma, and platelets vs. the control group. Mitochondrial CI-linked LEAK respiration was higher, and CI-linked oxidative phosphorylation (OXPHOS) and CII-linked electron transfer (ET) capacities were lower vs. the control group. Plasma TBARS concentrations were higher in the H<sub>2</sub> group. After 8 weeks of adjuvant therapy with HRW, the concentration of CoQ<sub>10</sub> in platelets increased, plasma TBARS decreased, and the efficiency of OXPHOS improved, while in the P group, the changes were non-significant. Long-term supplementation with HRW could be a promising strategy for the acceleration of health recovery in patients with NAFLD. The application of H<sub>2</sub> appears to be a new treatment strategy for targeted therapy of mitochondrial disorders. Additional and longer-term studies are needed to confirm and elucidate the exact mechanisms of the mitochondria-targeted effects of H<sub>2</sub> therapy in patients with NAFLD. |
| first_indexed | 2024-03-11T00:24:23Z |
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| id | doaj.art-5f0e3352f58649a5a8c04dedca32b60b |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:24:23Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5f0e3352f58649a5a8c04dedca32b60b2023-11-18T23:05:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124151247710.3390/ijms241512477The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver DiseaseZuzana Sumbalová0Jarmila Kucharská1Zuzana Rausová2Anna Gvozdjáková3Mária Szántová4Branislav Kura5Viliam Mojto6Ján Slezák7Pharmacobiochemical Laboratory of 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, SlovakiaPharmacobiochemical Laboratory of 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, SlovakiaPharmacobiochemical Laboratory of 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, SlovakiaPharmacobiochemical Laboratory of 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, Slovakia3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 813 72 Bratislava, SlovakiaInstitute for Heart Research, Center of Experimental Medicine, Slovak Academy of Sciences, 841 04 Bratislava, Slovakia3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, 813 72 Bratislava, SlovakiaInstitute for Heart Research, Center of Experimental Medicine, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaMolecular hydrogen (H<sub>2</sub>) has been recognized as a novel medical gas with antioxidant and anti-inflammatory effects. Non-alcoholic fatty liver disease (NAFLD) is a liver pathology with increased fat accumulation in liver tissue caused by factors other than alcohol consumption. Platelet mitochondrial function is considered to reflect systemic mitochondrial health. We studied the effect of adjuvant therapy with hydrogen-rich water (HRW) on coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) content and platelet mitochondrial bioenergetics in patients with NAFLD. A total of 30 patients with NAFLD and 15 healthy volunteers were included in this clinical trial. A total of 17 patients (H<sub>2</sub> group) drank water three × 330 mL/day with tablets producing HRW (>4 mg/L H<sub>2</sub>) for 8 weeks, and 13 patients (P group) drank water with placebo tablets producing CO<sub>2</sub>. The concentration of CoQ<sub>10-TOTAL</sub> was determined by the HPLC method, the parameter of oxidative stress, thiobarbituric acid reactive substances (TBARS), by the spectrophotometric method, and mitochondrial bioenergetics in platelets isolated from whole blood by high-resolution respirometry. The patients with NAFLD had lower concentrations of CoQ<sub>10-TOTAL</sub> in the blood, plasma, and platelets vs. the control group. Mitochondrial CI-linked LEAK respiration was higher, and CI-linked oxidative phosphorylation (OXPHOS) and CII-linked electron transfer (ET) capacities were lower vs. the control group. Plasma TBARS concentrations were higher in the H<sub>2</sub> group. After 8 weeks of adjuvant therapy with HRW, the concentration of CoQ<sub>10</sub> in platelets increased, plasma TBARS decreased, and the efficiency of OXPHOS improved, while in the P group, the changes were non-significant. Long-term supplementation with HRW could be a promising strategy for the acceleration of health recovery in patients with NAFLD. The application of H<sub>2</sub> appears to be a new treatment strategy for targeted therapy of mitochondrial disorders. Additional and longer-term studies are needed to confirm and elucidate the exact mechanisms of the mitochondria-targeted effects of H<sub>2</sub> therapy in patients with NAFLD.https://www.mdpi.com/1422-0067/24/15/12477molecular hydrogenplateletsmitochondriaoxidative phosphorylationcoenzyme Q<sub>10</sub> |
| spellingShingle | Zuzana Sumbalová Jarmila Kucharská Zuzana Rausová Anna Gvozdjáková Mária Szántová Branislav Kura Viliam Mojto Ján Slezák The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease International Journal of Molecular Sciences molecular hydrogen platelets mitochondria oxidative phosphorylation coenzyme Q<sub>10</sub> |
| title | The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease |
| title_full | The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease |
| title_fullStr | The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease |
| title_full_unstemmed | The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease |
| title_short | The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q<sub>10</sub> Levels and Platelet Mitochondrial Bioenergetics in Patients with Non-Alcoholic Fatty Liver Disease |
| title_sort | effect of adjuvant therapy with molecular hydrogen on endogenous coenzyme q sub 10 sub levels and platelet mitochondrial bioenergetics in patients with non alcoholic fatty liver disease |
| topic | molecular hydrogen platelets mitochondria oxidative phosphorylation coenzyme Q<sub>10</sub> |
| url | https://www.mdpi.com/1422-0067/24/15/12477 |
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